##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921192_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2605063
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.821805077266845	31.0	30.0	33.0	26.0	34.0
2	30.055333786553337	31.0	30.0	34.0	26.0	34.0
3	30.179855151295765	31.0	30.0	34.0	26.0	34.0
4	33.87833077357438	35.0	35.0	37.0	30.0	37.0
5	33.49803824322099	35.0	35.0	37.0	28.0	37.0
6	33.49003191093651	37.0	35.0	37.0	28.0	37.0
7	33.35531847022509	37.0	35.0	37.0	28.0	37.0
8	33.30602714790391	37.0	35.0	37.0	27.0	37.0
9	34.69410298330597	39.0	35.0	39.0	27.0	39.0
10	34.57490740147167	38.0	35.0	39.0	27.0	39.0
11	34.62257764975357	39.0	35.0	39.0	27.0	39.0
12	34.47671438272318	38.0	35.0	39.0	27.0	39.0
13	34.46252048414952	38.0	35.0	39.0	27.0	39.0
14	35.43455570940127	39.0	35.0	41.0	27.0	41.0
15	35.43415763841412	39.0	35.0	41.0	27.0	41.0
16	35.41777185426994	39.0	35.0	41.0	27.0	41.0
17	35.40625005997936	39.0	36.0	41.0	27.0	41.0
18	35.363295244683144	39.0	35.0	41.0	27.0	41.0
19	35.40872907872094	39.0	35.0	41.0	26.0	41.0
20	35.350018022596764	39.0	35.0	41.0	26.0	41.0
21	35.27968997294883	39.0	35.0	41.0	26.0	41.0
22	35.19919978902622	39.0	35.0	41.0	25.0	41.0
23	35.07494367698593	39.0	35.0	41.0	25.0	41.0
24	35.0016920895963	39.0	35.0	41.0	25.0	41.0
25	34.86703469359474	39.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	70916.0
3	5.0
4	1088.0
5	9533.0
6	10924.0
7	8397.0
8	6537.0
9	5621.0
10	4939.0
11	4761.0
12	4415.0
13	4226.0
14	4147.0
15	4069.0
16	4138.0
17	4391.0
18	4860.0
19	6010.0
20	7641.0
21	9652.0
22	12384.0
23	15028.0
24	17856.0
25	20683.0
26	24328.0
27	27556.0
28	31766.0
29	38961.0
30	49471.0
31	64100.0
32	82506.0
33	107519.0
34	141685.0
35	189287.0
36	259435.0
37	393619.0
38	952609.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.135867499801144	18.728041743311223	9.476782992190614	30.65930776469702
2	14.7546143797674	20.877345384737335	35.78738786739514	28.580652368100118
3	18.348530489294145	24.154261373673386	27.814174829761644	29.683033307270822
4	13.126753076561748	16.229259301507852	33.61609164411168	37.02789597781872
5	14.417236120874097	37.990297191716884	32.4322704801853	15.160196207223715
6	33.988205166985985	35.03713380106785	16.51453202811935	14.460129003826813
7	29.99405388660466	32.096191147776466	19.23523538586207	18.674519579756804
8	28.017461442258014	34.70254359410592	18.47121019372343	18.80878476991264
9	27.653381127442984	15.392871496773783	18.63563376394352	38.31811361183971
10	16.696410029239217	28.88594248968259	30.99433679722909	23.423310683849106
11	36.039019491704025	22.551445131526872	21.51142950039097	19.898105876378136
12	24.063602300596955	25.17086918819238	28.407028927899248	22.35849958331142
13	29.0692728766324	21.027461939456288	25.157386010303025	24.745879173608284
14	23.05504825003601	20.65785203322291	25.74161025493303	30.545489461808057
15	25.163670347129724	27.817950118891616	22.545559120789505	24.472820413189154
16	24.68830888158943	27.166751821357103	24.256956549611274	23.887982747442194
17	23.52676307636322	27.14759681435727	25.565331817311137	23.76030829196837
18	23.41202253000796	26.72749446365706	26.635366032016933	23.22511697431805
19	24.720610719842213	26.06994779768734	26.445563379227405	22.763878103243037
20	25.035709309141467	25.46226329267277	26.28205152812043	23.21997587006533
21	25.159473454488502	25.175481261077344	26.191689069278485	23.47335621515567
22	25.073059653451757	25.101964904495595	25.720721533413972	24.10425390863868
23	24.455636338510438	25.22901583840679	25.9701887864387	24.34515903664407
24	24.0957015160507	25.732901148458115	26.12437035950831	24.047026975982877
25	24.16952537770171	25.30736991791292	26.244063231701524	24.27904147268385
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	785.0
1	785.0
2	807.5
3	830.0
4	830.0
5	830.0
6	1323.0
7	1816.0
8	1816.0
9	1816.0
10	2427.0
11	3038.0
12	3038.0
13	3038.0
14	4301.5
15	5565.0
16	5565.0
17	5565.0
18	10904.5
19	16244.0
20	16244.0
21	16244.0
22	27329.0
23	38414.0
24	38414.0
25	38414.0
26	57598.5
27	76783.0
28	76783.0
29	76783.0
30	99394.0
31	122005.0
32	122005.0
33	122005.0
34	147480.5
35	172956.0
36	172956.0
37	172956.0
38	202440.0
39	231924.0
40	231924.0
41	231924.0
42	260578.5
43	289233.0
44	289233.0
45	289233.0
46	321433.0
47	353633.0
48	353633.0
49	353633.0
50	362262.5
51	370892.0
52	370892.0
53	370892.0
54	346501.0
55	322110.0
56	322110.0
57	322110.0
58	285736.0
59	249362.0
60	249362.0
61	249362.0
62	210825.5
63	172289.0
64	172289.0
65	172289.0
66	135061.5
67	97834.0
68	97834.0
69	97834.0
70	72595.5
71	47357.0
72	47357.0
73	47357.0
74	33120.5
75	18884.0
76	18884.0
77	18884.0
78	13549.5
79	8215.0
80	8215.0
81	8215.0
82	5762.5
83	3310.0
84	3310.0
85	3310.0
86	2222.5
87	1135.0
88	1135.0
89	1135.0
90	759.5
91	384.0
92	384.0
93	384.0
94	220.0
95	56.0
96	56.0
97	56.0
98	32.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10295336427564322
2	0.0
3	0.04095870234232339
4	0.054739559081680565
5	0.0649888313641551
6	9.212829017954653E-4
7	0.0
8	3.4548108817329947E-4
9	0.0
10	0.0
11	2.3032072544886632E-4
12	0.0
13	1.1516036272443316E-4
14	0.05520020053257829
15	0.02264820466913852
16	0.0
17	0.0
18	1.5354715029924419E-4
19	2.3032072544886632E-4
20	0.0
21	0.003224490156284128
22	0.0
23	1.1516036272443316E-4
24	1.9193393787405523E-4
25	0.17396892128904368
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2605063.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.01449186948409
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.22240383645602	28.055085595314456
2	15.598024700620543	13.418822133297088
3	7.116385500657016	9.18323118774377
4	3.7600005440601616	6.469380513269263
5	2.264200924445894	4.869672612772813
6	1.4009818997314363	3.6157514721175343
7	0.9825243047669654	2.9583948603278394
8	0.67166542332215	2.3113077512403364
9	0.481624009599393	1.8645130840555277
>10	2.3292007431313646	17.458111350567712
>50	0.11800540114471057	3.453596064509188
>100	0.0509407089469761	3.8967069071061484
>500	0.002335379578946674	0.6937974670219519
>1k	0.0016168012469546624	1.4610431149598617
>5k	8.982229149748124E-5	0.2905858856964993
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7521	0.2887070293501539	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4757	0.18260594849337616	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4429	0.17001508216883815	No Hit
CCCATGTACTCTGCGTTGATACCAC	3795	0.14567785884640794	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3009	0.11550584381260645	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2826	0.10848106168641602	No Hit
GGTATCAACGCAGAGTACATGGGGG	2762	0.10602430728162812	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.67735751496221E-5
2	0.0	0.0	0.0	0.0	7.67735751496221E-5
3	0.0	0.0	0.0	0.0	7.67735751496221E-5
4	0.0	0.0	0.0	0.0	7.67735751496221E-5
5	0.0	0.0	0.0	0.0	1.1516036272443315E-4
6	0.0	0.0	0.0	0.0	1.9193393787405525E-4
7	0.0	0.0	0.0	0.0	1.9193393787405525E-4
8	0.0	0.0	0.0	0.0	1.9193393787405525E-4
9	0.0	0.0	0.0	0.0	1.9193393787405525E-4
10	0.0	0.0	0.0	0.0	2.6870751302367733E-4
11	0.0	3.838678757481105E-5	0.0	0.0	2.6870751302367733E-4
12	0.0	3.838678757481105E-5	0.0	0.0	9.596696893702763E-4
13	0.0	3.838678757481105E-5	0.0	0.0	0.0011899904148191425
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAC	60	4.0823425E-4	12.67083	3
CGATACT	60	4.0823425E-4	12.67083	4
TAATACT	255	0.0	11.180143	4
ACGCAAT	70	0.0014972308	10.852579	15
TTACCCT	135	5.6648787E-8	10.559025	4
GGTATCA	2690	0.0	10.5037985	1
TTATACT	230	0.0	10.32948	4
GTCCTAC	120	1.497101E-6	10.306336	1
CCCCTAT	140	9.496398E-8	10.19308	1
TTAATAC	290	0.0	10.15851	3
TAGGGTG	310	0.0	10.11234	5
CTATAAG	285	0.0	10.014254	1
GTATCAA	5165	0.0	9.872716	1
GTACTAA	155	3.977948E-8	9.82043	1
GTCTTAG	320	0.0	9.81084	1
GATCTCG	165	1.0069925E-8	9.797308	19
TATAAGG	255	0.0	9.689458	2
ACTATAC	190	1.0459189E-9	9.503122	3
TCTATAC	130	4.223124E-6	9.503121	3
CCATGCG	80	0.0045128106	9.501114	9
>>END_MODULE