FastQCFastQC Report
Mon 15 Aug 2016
SRR2921191_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921191_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866528
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34030.18231711498568465No Hit
TATCAACGCAGAGTACTTTTTTTTT19490.10441847108642356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC1101.8553692E-1013.8194881
TCTAGCG500.001504437113.2944883
GGTATCA17050.011.924881
GTTATCA1850.011.2983661
CGATTCG600.00588987111.0799269
CTAGCGT600.005895746411.0784424
GTACCGG1053.4628993E-610.858171
AAGACCG1451.4533725E-810.4781325
GTATCAA34900.010.3448171
CGCATAG1201.5298647E-610.2885038
ACGCATA1303.8579128E-710.2276247
TCTATAC1409.747782E-810.1743523
ACGATTC856.612928E-410.0557328
TACACAG4300.09.9374735
CTATTAG1252.566927E-69.8809341
GTCTTAG1752.568413E-99.7723521
GTATATG1856.475602E-109.7576791
CTATACT1951.6552804E-109.7392894
GTCCTAG2054.1836756E-119.7326271
TCTACAC3050.09.6517353
Produced by FastQC (version 0.11.5)