##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921191_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1866528
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.88130904010012	31.0	27.0	31.0	7.0	34.0
2	26.6115461434278	31.0	27.0	31.0	7.0	34.0
3	26.23709850588901	31.0	26.0	31.0	7.0	34.0
4	30.071330298822197	35.0	30.0	37.0	7.0	37.0
5	29.90062779663632	35.0	30.0	37.0	7.0	37.0
6	30.137182512129474	35.0	30.0	37.0	2.0	37.0
7	30.135817946476024	35.0	30.0	37.0	2.0	37.0
8	29.765016651236948	35.0	30.0	37.0	2.0	37.0
9	31.314060115894325	37.0	31.0	39.0	2.0	39.0
10	30.673825948499033	37.0	30.0	39.0	2.0	39.0
11	31.138802096727186	37.0	30.0	39.0	2.0	39.0
12	30.84255794716179	37.0	30.0	39.0	2.0	39.0
13	30.841864145622246	37.0	30.0	39.0	2.0	39.0
14	31.914941538514288	38.0	31.0	40.0	2.0	41.0
15	31.570488093401224	38.0	30.0	40.0	2.0	41.0
16	31.489253308817226	38.0	30.0	40.0	2.0	41.0
17	31.805542697457525	38.0	31.0	40.0	2.0	41.0
18	31.85353072656826	38.0	31.0	40.0	2.0	41.0
19	31.555264641087625	38.0	30.0	40.0	2.0	41.0
20	31.429628700989216	38.0	30.0	40.0	2.0	41.0
21	31.364534579711634	38.0	30.0	40.0	2.0	41.0
22	30.609166323784052	38.0	28.0	40.0	2.0	41.0
23	30.700855813574723	38.0	29.0	40.0	2.0	41.0
24	30.331109418128204	37.0	28.0	40.0	2.0	41.0
25	29.842710637075896	37.0	27.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	173604.0
3	12.0
4	1238.0
5	7484.0
6	9212.0
7	8138.0
8	6679.0
9	6017.0
10	5670.0
11	5452.0
12	5290.0
13	5134.0
14	5310.0
15	5332.0
16	5607.0
17	6422.0
18	7270.0
19	8911.0
20	11249.0
21	13799.0
22	16875.0
23	19484.0
24	22251.0
25	25218.0
26	28129.0
27	31130.0
28	34659.0
29	41019.0
30	48582.0
31	57354.0
32	71019.0
33	90074.0
34	114897.0
35	147501.0
36	193772.0
37	255197.0
38	371537.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.503367833439086	17.70835119792828	12.320568008369206	25.467712960263423
2	15.980582035058905	19.674948498758233	39.12823929811432	25.21623016806853
3	18.958398079581624	24.125202276210175	29.40115525167983	27.51524439252837
4	14.157640028976687	15.932622775846475	34.29756184336301	35.61217535181383
5	14.115366418606595	36.56309725565797	33.865370303196244	15.456166022539191
6	33.46137938276447	34.79134387275544	16.67030981786387	15.076966926616219
7	29.442439451965548	31.876844408535554	19.583787849888452	19.09692828961045
8	26.85704978671104	33.70940625197564	20.238395012649466	19.19514894866385
9	27.401997947937623	16.003461180927072	18.904691614673048	37.68984925646225
10	16.530169850401773	27.995795262276406	31.953917188954957	23.52011769836686
11	35.334193130800486	22.99576013043774	21.38382741325092	20.286219325510853
12	23.670643404198774	25.198167121564293	28.362786481021285	22.76840299321565
13	28.83801375514639	21.630224887427694	24.769443771713433	24.76231758571248
14	23.257347945275946	21.49524497437623	25.220711635613075	30.026695444734752
15	24.93194062318332	27.902013786008784	22.88343508975908	24.28261050104882
16	24.333033753870016	27.425562006249905	24.431375134047073	23.81002910583301
17	23.428050158325885	27.46980874404804	25.22173127263138	23.880409824994693
18	23.3429238834708	26.78850184317548	26.394787342188646	23.473786931165073
19	24.559331561103612	26.447286704151058	26.63804878257892	22.35533295216641
20	25.416820945563973	25.486802389228647	26.029828384709326	23.066548280498058
21	25.743737812024236	25.494820428480864	25.387268827023053	23.374172932471847
22	24.650168961546846	24.959105787020043	26.608161911614918	23.782563339818186
23	24.750444348111714	25.34807045482921	25.681954456528906	24.219530740530175
24	24.574032071303378	25.598209056630868	25.85636981394529	23.97138905812046
25	23.96858992715912	25.28766011321234	26.53513824264647	24.208611716982066
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1834.0
1	1834.0
2	1917.0
3	2000.0
4	2000.0
5	2000.0
6	2475.0
7	2950.0
8	2950.0
9	2950.0
10	3391.0
11	3832.0
12	3832.0
13	3832.0
14	5064.5
15	6297.0
16	6297.0
17	6297.0
18	10195.5
19	14094.0
20	14094.0
21	14094.0
22	22386.0
23	30678.0
24	30678.0
25	30678.0
26	45549.5
27	60421.0
28	60421.0
29	60421.0
30	77645.0
31	94869.0
32	94869.0
33	94869.0
34	112796.5
35	130724.0
36	130724.0
37	130724.0
38	149666.0
39	168608.0
40	168608.0
41	168608.0
42	186101.5
43	203595.0
44	203595.0
45	203595.0
46	221399.5
47	239204.0
48	239204.0
49	239204.0
50	245040.0
51	250876.0
52	250876.0
53	250876.0
54	236237.5
55	221599.0
56	221599.0
57	221599.0
58	199408.5
59	177218.0
60	177218.0
61	177218.0
62	149879.5
63	122541.0
64	122541.0
65	122541.0
66	96670.0
67	70799.0
68	70799.0
69	70799.0
70	53665.0
71	36531.0
72	36531.0
73	36531.0
74	26342.5
75	16154.0
76	16154.0
77	16154.0
78	11823.5
79	7493.0
80	7493.0
81	7493.0
82	5125.5
83	2758.0
84	2758.0
85	2758.0
86	1915.5
87	1073.0
88	1073.0
89	1073.0
90	700.0
91	327.0
92	327.0
93	327.0
94	186.0
95	45.0
96	45.0
97	45.0
98	26.5
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034288261413705015
2	0.05566484938881174
3	0.014358209466988977
4	0.010715081691782818
5	0.009589998114145622
6	6.42904901506969E-4
7	0.004071731042877471
8	0.002464468789110048
9	0.006054021155857292
10	0.003321675324452674
11	0.010554355466406077
12	0.009322121071851052
13	0.009214970254933225
14	0.015644019270002914
15	0.007661283409624716
16	0.03102016149771126
17	0.04050300879493905
18	0.02437681084880591
19	0.017626309382982736
20	0.017090555298393595
21	0.023948207581134598
22	0.0068040768742820895
23	0.016661952030722283
24	0.01334027670626961
25	0.4703920862692657
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1866528.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.64790690112007
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.0472662513096	35.04948701073919
2	11.541144667562014	11.229050626398305
3	5.614227630998885	8.19361269343589
4	3.1802519151811133	6.188503963673585
5	2.00241193715938	4.87065747483105
6	1.3317583474750245	3.88723536616527
7	0.9105718552729007	3.100819038946738
8	0.6683380599663853	2.6010598175775934
9	0.4818852395577048	2.109843744392445
>10	2.118414851419279	16.760474324268717
>50	0.07231632934961475	2.398017322994053
>100	0.02912883396601633	2.343185686219259
>500	0.0013704484692659375	0.5136881087490933
>1k	9.136323128439583E-4	0.7543648216088242
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3403	0.18231711498568465	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1949	0.10441847108642356	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.357540845891409E-5
2	0.0	0.0	0.0	0.0	5.357540845891409E-5
3	0.0	0.0	0.0	0.0	5.357540845891409E-5
4	0.0	0.0	0.0	0.0	5.357540845891409E-5
5	0.0	0.0	0.0	0.0	5.357540845891409E-5
6	0.0	0.0	0.0	0.0	5.357540845891409E-5
7	0.0	0.0	0.0	0.0	5.357540845891409E-5
8	0.0	0.0	0.0	0.0	5.357540845891409E-5
9	0.0	0.0	0.0	0.0	5.357540845891409E-5
10	0.0	0.0	0.0	0.0	5.357540845891409E-5
11	0.0	0.0	0.0	0.0	5.357540845891409E-5
12	0.0	0.0	0.0	5.357540845891409E-5	1.0715081691782819E-4
13	0.0	0.0	0.0	5.357540845891409E-5	1.0715081691782819E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTTAC	110	1.8553692E-10	13.819488	1
TCTAGCG	50	0.0015044371	13.294488	3
GGTATCA	1705	0.0	11.92488	1
GTTATCA	185	0.0	11.298366	1
CGATTCG	60	0.005889871	11.079926	9
CTAGCGT	60	0.0058957464	11.078442	4
GTACCGG	105	3.4628993E-6	10.85817	1
AAGACCG	145	1.4533725E-8	10.478132	5
GTATCAA	3490	0.0	10.344817	1
CGCATAG	120	1.5298647E-6	10.288503	8
ACGCATA	130	3.8579128E-7	10.227624	7
TCTATAC	140	9.747782E-8	10.174352	3
ACGATTC	85	6.612928E-4	10.055732	8
TACACAG	430	0.0	9.937473	5
CTATTAG	125	2.566927E-6	9.880934	1
GTCTTAG	175	2.568413E-9	9.772352	1
GTATATG	185	6.475602E-10	9.757679	1
CTATACT	195	1.6552804E-10	9.739289	4
GTCCTAG	205	4.1836756E-11	9.732627	1
TCTACAC	305	0.0	9.651735	3
>>END_MODULE