FastQCFastQC Report
Mon 15 Aug 2016
SRR2921191_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921191_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866528
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63340.33934663717876185No Hit
GGTATCAACGCAGAGTACTTTTTTT45260.24248229868504517No Hit
TATCAACGCAGAGTACTTTTTTTTT42930.22999922851411816No Hit
CTTATACACATCTCCGAGCCCACGA31260.16747672684256545No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30590.16388717447581821No Hit
GGTATCAACGCAGAGTACATGGGGG26030.13945678821855337No Hit
GGTATCAACGCAGAGTACATGGGGA19440.104150594044129No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCGT402.7659212E-416.6215048
CTTATAC3900.014.8853761
CGTCCCA1300.014.61505410
TACGGGA551.9506252E-413.8263952
TTATACA4700.013.3483542
GGTATCA20100.012.9259111
ACGGGAC905.368074E-712.6741953
TATACAC5000.012.5474533
ACACCGT1850.012.3151326
GTGTAAC657.906114E-411.7130821
GATTAAG1001.8892861E-611.4202561
CCCTAGA2350.011.339261
TCTCGGA855.3251773E-511.17621813
GCTTAGT600.00579926511.1030251
ATTAGAT600.005850473411.0899213
CGACCCG951.36002E-511.0003415
CGTGCGC700.001491925610.85689810
TCGTCCC700.001491925610.8568989
CACCGTT700.001500958210.8490467
CTTACAC2750.010.7154563
Produced by FastQC (version 0.11.5)