##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921191_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1866528
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.528059584426273	31.0	30.0	33.0	26.0	34.0
2	29.76749665689451	31.0	30.0	34.0	26.0	34.0
3	29.88625619331722	31.0	30.0	34.0	26.0	34.0
4	33.57757344116992	35.0	35.0	37.0	28.0	37.0
5	33.143692460011316	35.0	35.0	37.0	27.0	37.0
6	33.115453933720794	36.0	35.0	37.0	27.0	37.0
7	32.97754976083937	36.0	35.0	37.0	26.0	37.0
8	32.920358012309485	36.0	35.0	37.0	26.0	37.0
9	34.27373497745547	38.0	35.0	39.0	26.0	39.0
10	34.133967987621936	38.0	34.0	39.0	25.0	39.0
11	34.17995229645631	38.0	35.0	39.0	25.0	39.0
12	34.04449598398738	38.0	34.0	39.0	25.0	39.0
13	34.02158017452725	38.0	34.0	39.0	25.0	39.0
14	34.96285563356135	39.0	34.0	41.0	25.0	41.0
15	34.95883426340242	39.0	34.0	41.0	25.0	41.0
16	34.93374168509661	39.0	34.0	41.0	25.0	41.0
17	34.9300535539783	39.0	35.0	41.0	25.0	41.0
18	34.88374511392275	39.0	35.0	41.0	24.0	41.0
19	34.91391985547498	39.0	35.0	41.0	24.0	41.0
20	34.8484571353872	39.0	34.0	41.0	24.0	41.0
21	34.77261739443502	39.0	34.0	41.0	23.0	41.0
22	34.68297448524748	39.0	34.0	41.0	22.0	41.0
23	34.556878868144494	39.0	34.0	41.0	19.0	41.0
24	34.46953755850435	39.0	34.0	41.0	18.0	41.0
25	34.32811508854944	39.0	34.0	41.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	60692.0
3	6.0
4	966.0
5	8137.0
6	9185.0
7	7131.0
8	5606.0
9	4617.0
10	4262.0
11	3891.0
12	3610.0
13	3411.0
14	3276.0
15	3341.0
16	3526.0
17	3684.0
18	4056.0
19	4904.0
20	6073.0
21	7849.0
22	9985.0
23	11961.0
24	14013.0
25	16123.0
26	18248.0
27	20894.0
28	23797.0
29	29264.0
30	36906.0
31	46912.0
32	60048.0
33	77729.0
34	101878.0
35	134649.0
36	182832.0
37	275707.0
38	657359.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.68650138391102	18.9649707745665	9.572878025445085	30.775649816077394
2	15.134945738826314	20.954253030225104	35.096071422448524	28.81472980850006
3	18.335247648822172	23.849387410110694	27.92379755885917	29.891567382207963
4	13.58406152219839	16.44092409807057	32.632688867649115	37.34232551208192
5	14.784275252768934	37.48919767977956	32.005371674868925	15.721155392582586
6	34.16220149039824	34.766815199297504	15.926012640900176	15.144970669404081
7	29.809734899686635	32.149674712202135	18.934894757624026	19.105695630487205
8	27.743816193835695	34.35880648576364	18.506039149461447	19.391338170939218
9	27.503643143389837	15.537952982020014	18.7002014443992	38.25820243019095
10	17.413580094785715	28.44080393201059	30.241153886953626	23.90446208625007
11	35.56251098319411	22.62162949882346	21.23983652721046	20.576022990771975
12	23.940196921982334	25.154163403027873	27.767735354342776	23.13790432064702
13	29.099233909227433	21.02862647462696	24.822273262794475	25.04986635335113
14	23.295739617058686	21.053571045691374	25.158611891335575	30.49207744591436
15	25.20097914704032	27.6547413502069	22.449753873557565	24.694525629195216
16	24.942592904129345	26.9865319684928	23.802916055449412	24.267959071928438
17	23.38128378631778	27.31959646613198	25.26704919877204	24.0320705487782
18	23.58927568781583	26.314661546176566	26.59181307353704	23.504249692470566
19	24.777710866611518	26.016347278953845	26.467515462194243	22.7384263922404
20	25.283671889440424	25.242364740517704	26.10338573965781	23.37057763038406
21	25.5659638396067	25.020707627586308	25.76323546903178	23.650093063775206
22	25.278422031383858	25.074136043859607	25.468671058842183	24.178770865914352
23	24.585518585965414	24.970894551213537	25.84954960891813	24.59403725390292
24	24.638894008426522	25.347336816523867	25.851010025245273	24.162759149804337
25	24.333077182310127	25.1621156929708	26.080610297700435	24.424196827018637
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1247.0
1	1247.0
2	1325.5
3	1404.0
4	1404.0
5	1404.0
6	1718.0
7	2032.0
8	2032.0
9	2032.0
10	2415.0
11	2798.0
12	2798.0
13	2798.0
14	3601.5
15	4405.0
16	4405.0
17	4405.0
18	8195.0
19	11985.0
20	11985.0
21	11985.0
22	20154.0
23	28323.0
24	28323.0
25	28323.0
26	42980.5
27	57638.0
28	57638.0
29	57638.0
30	72872.5
31	88107.0
32	88107.0
33	88107.0
34	104758.5
35	121410.0
36	121410.0
37	121410.0
38	140242.5
39	159075.0
40	159075.0
41	159075.0
42	176003.5
43	192932.0
44	192932.0
45	192932.0
46	213209.5
47	233487.0
48	233487.0
49	233487.0
50	246044.5
51	258602.0
52	258602.0
53	258602.0
54	244877.0
55	231152.0
56	231152.0
57	231152.0
58	209937.0
59	188722.0
60	188722.0
61	188722.0
62	161256.5
63	133791.0
64	133791.0
65	133791.0
66	105609.0
67	77427.0
68	77427.0
69	77427.0
70	58896.5
71	40366.0
72	40366.0
73	40366.0
74	29140.0
75	17914.0
76	17914.0
77	17914.0
78	13185.0
79	8456.0
80	8456.0
81	8456.0
82	5912.5
83	3369.0
84	3369.0
85	3369.0
86	2361.5
87	1354.0
88	1354.0
89	1354.0
90	901.5
91	449.0
92	449.0
93	449.0
94	260.0
95	71.0
96	71.0
97	71.0
98	41.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10098964494505305
2	0.0
3	0.04077088583723362
4	0.056522055924154366
5	0.06364758524918994
6	0.002518044197568962
7	0.0014465360283906804
8	0.0021430163383565634
9	4.286032676713127E-4
10	1.0715081691782817E-4
11	0.0021430163383565634
12	1.0715081691782817E-4
13	0.0020894409298976495
14	0.051860995388228845
15	0.02218021910199043
16	0.0018215638876030791
17	9.643573522604536E-4
18	0.0021430163383565634
19	0.0021430163383565634
20	0.001232234394555024
21	0.00353597695828833
22	0.0014465360283906804
23	0.0020894409298976495
24	0.0021430163383565634
25	0.1630835433489345
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1866528.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.346482922264705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.00862749642329	20.610852994944466
2	14.142942887291406	9.715206926978388
3	7.516818072567433	7.745287906776241
4	4.655793203067431	6.396404869550065
5	3.154010494257384	5.416458378882744
6	2.1641036223474286	4.459760886418029
7	1.590129985883563	3.823076068303156
8	1.2149703412605866	3.338396646173188
9	0.8972177990181534	2.773464823037604
>10	4.417671824295349	25.2511783096574
>50	0.16736124868482993	3.9227242513111062
>100	0.06483252179591646	3.8272615017351783
>500	0.0033123018642946638	0.8341208863041143
>1k	0.0020504725826586013	1.542665867272583
>5k	1.577286602045078E-4	0.34313968265572903
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6334	0.33934663717876185	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4526	0.24248229868504517	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4293	0.22999922851411816	No Hit
CTTATACACATCTCCGAGCCCACGA	3126	0.16747672684256545	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3059	0.16388717447581821	No Hit
GGTATCAACGCAGAGTACATGGGGG	2603	0.13945678821855337	No Hit
GGTATCAACGCAGAGTACATGGGGA	1944	0.104150594044129	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.357540845891409E-5
2	0.0	0.0	0.0	0.0	5.357540845891409E-5
3	0.0	0.0	0.0	0.0	5.357540845891409E-5
4	0.0	0.0	0.0	0.0	5.357540845891409E-5
5	0.0	0.0	0.0	0.0	5.357540845891409E-5
6	0.0	0.0	0.0	0.0	5.357540845891409E-5
7	0.0	0.0	0.0	0.0	5.357540845891409E-5
8	0.0	0.0	0.0	0.0	5.357540845891409E-5
9	0.0	0.0	0.0	0.0	5.357540845891409E-5
10	0.0	0.0	0.0	0.0	5.357540845891409E-5
11	0.0	0.0	0.0	0.0	5.357540845891409E-5
12	0.0	0.0	0.0	5.357540845891409E-5	5.357540845891409E-5
13	0.0	0.0	0.0	5.357540845891409E-5	5.357540845891409E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGCGT	40	2.7659212E-4	16.621504	8
CTTATAC	390	0.0	14.885376	1
CGTCCCA	130	0.0	14.615054	10
TACGGGA	55	1.9506252E-4	13.826395	2
TTATACA	470	0.0	13.348354	2
GGTATCA	2010	0.0	12.925911	1
ACGGGAC	90	5.368074E-7	12.674195	3
TATACAC	500	0.0	12.547453	3
ACACCGT	185	0.0	12.315132	6
GTGTAAC	65	7.906114E-4	11.713082	1
GATTAAG	100	1.8892861E-6	11.420256	1
CCCTAGA	235	0.0	11.33926	1
TCTCGGA	85	5.3251773E-5	11.176218	13
GCTTAGT	60	0.005799265	11.103025	1
ATTAGAT	60	0.0058504734	11.089921	3
CGACCCG	95	1.36002E-5	11.000341	5
CGTGCGC	70	0.0014919256	10.856898	10
TCGTCCC	70	0.0014919256	10.856898	9
CACCGTT	70	0.0015009582	10.849046	7
CTTACAC	275	0.0	10.715456	3
>>END_MODULE