FastQCFastQC Report
Mon 15 Aug 2016
SRR2921190_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921190_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2396116
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27670.11547854945253068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG701.09572735E-412.209085
GGTATCA14250.011.4677631
CGATTCG600.00589133311.0797669
TAGACCG803.787167E-410.6829455
GTATCAA29350.010.1645151
GACCGTG1451.6255945E-79.8244237
GTCCTAT1951.6370905E-109.7445211
GTCTTAG2950.09.6619411
GTCCTAG2900.09.5009091
GCTTAGG2002.6375346E-109.5009081
GTATAAT3600.09.5009081
TGATCGA800.00452428829.49813310
GACGCTC900.00111726179.4969428
TAGGACC2054.274625E-109.2643434
AGGCCGT1252.766781E-59.1166846
TGCCGGT1054.3244511E-49.08243119
TAAGGCT2201.6916601E-109.0643184
CGAATTT950.00182608758.99898115
ATAATAC1804.2466127E-88.9687733
GGTATAT1606.7336987E-78.9071021
Produced by FastQC (version 0.11.5)