FastQCFastQC Report
Mon 15 Aug 2016
SRR2921190_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921190_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2396116
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48720.20332905418602437No Hit
GGTATCAACGCAGAGTACTTTTTTT33410.13943398399743587No Hit
TATCAACGCAGAGTACTTTTTTTTT33350.13918357875829052No Hit
GGTATCAACGCAGAGTACATGGGGG24040.10032903248423698No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGA655.4904885E-513.1447318
CGTGCGC1001.4433681E-712.350203510
GCACCGT1001.9484596E-611.3913866
CCATGCG855.324521E-511.1766559
GGTATCA17850.011.1390181
TCGAGTG700.001491707710.85732213
GTATCAA33900.010.3553691
CTAGATC750.002635740710.1392183
CCTACGT750.002635740710.1392182
AGGGCGC1502.4458132E-810.13350110
CGTCCCA951.6443928E-410.00016510
GCGTGCG1151.0266811E-59.9132069
TTATACT2900.09.8332934
AGGACTG4700.09.7018825
CCCTTAG1401.0387757E-69.5134681
ATTATAC2500.09.5055173
CCTATTC1701.6500053E-89.5055163
TAGACAG3000.09.499765
TAATCCC2750.09.3270375
CTGCGTT3700.09.24339611
Produced by FastQC (version 0.11.5)