##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921190_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2396116
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.543064275686152	31.0	30.0	33.0	26.0	34.0
2	29.82541162447895	31.0	30.0	34.0	26.0	34.0
3	29.97912997534343	31.0	30.0	34.0	26.0	34.0
4	33.64527134746398	35.0	35.0	37.0	30.0	37.0
5	33.15283608973856	35.0	35.0	37.0	27.0	37.0
6	33.12284964500884	36.0	35.0	37.0	27.0	37.0
7	32.99621178607379	36.0	35.0	37.0	26.0	37.0
8	32.94446554340441	37.0	35.0	37.0	26.0	37.0
9	34.32635273083607	39.0	35.0	39.0	26.0	39.0
10	34.20316086533373	38.0	35.0	39.0	25.0	39.0
11	34.22927729709246	38.0	35.0	39.0	26.0	39.0
12	34.09652996766434	38.0	35.0	39.0	25.0	39.0
13	34.06254663797579	38.0	35.0	39.0	25.0	39.0
14	35.03574075712528	39.0	35.0	41.0	25.0	41.0
15	35.027369709980654	39.0	35.0	41.0	25.0	41.0
16	35.00408577881872	39.0	35.0	41.0	25.0	41.0
17	34.98622353842635	39.0	35.0	41.0	25.0	41.0
18	34.94297980565215	39.0	35.0	41.0	25.0	41.0
19	34.982739984207775	39.0	35.0	41.0	24.0	41.0
20	34.92063239008462	39.0	35.0	41.0	24.0	41.0
21	34.8452816975472	39.0	35.0	41.0	23.0	41.0
22	34.75386417018208	39.0	35.0	41.0	22.0	41.0
23	34.63183543701557	39.0	34.0	41.0	19.0	41.0
24	34.55628734168129	39.0	34.0	41.0	19.0	41.0
25	34.41638802128111	39.0	34.0	41.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	79489.0
3	1.0
4	1362.0
5	10658.0
6	12000.0
7	9028.0
8	7094.0
9	6200.0
10	5352.0
11	4981.0
12	4564.0
13	4352.0
14	4316.0
15	4096.0
16	4249.0
17	4547.0
18	5031.0
19	6002.0
20	7524.0
21	9605.0
22	12146.0
23	14704.0
24	17549.0
25	20208.0
26	22982.0
27	25620.0
28	29845.0
29	35884.0
30	46115.0
31	57639.0
32	74253.0
33	97127.0
34	127419.0
35	168445.0
36	231499.0
37	353626.0
38	870604.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.68555285172691	19.500736300118014	9.562418406458553	31.25129244169652
2	14.681050500059262	21.108368710029062	35.64589527385152	28.564685516060155
3	17.741952319100708	23.932386161759002	28.462201023660082	29.863460495480204
4	13.214037848171357	16.96903946422263	33.80516184315032	36.011760844455694
5	14.432080812603	37.557288060278694	32.88862958857839	15.122001538539923
6	33.24817908185326	35.698885329317825	16.669393383427014	14.383542205401904
7	29.235599489673486	32.2190981810958	19.754577517838264	18.79072481139245
8	26.968247118401578	35.53453869801583	18.6749864152323	18.822227768350288
9	26.757803044593835	16.11332673376414	19.1448160272708	37.984054194371225
10	16.36757151990972	29.124883770234828	31.39885548112028	23.10868922873517
11	35.224756779722206	23.02841433859234	21.999316383140957	19.7475124985445
12	23.21970221808961	25.293266269245727	29.02213415377219	22.464897358892475
13	28.552254596522275	21.615037738329498	25.184163465290542	24.648544199857685
14	22.49248854228653	21.156566131103386	26.042323070980405	30.30862225562968
15	24.30863557980939	28.574350608555974	22.864481344671393	24.25253246696324
16	24.20592192685484	27.654842511535353	24.571222046381806	23.568013515228003
17	22.84743309589352	27.746319460326628	25.9478672985782	23.45838014520165
18	22.922647822452376	27.406996548927886	26.702550471251428	22.967805157368304
19	24.01872404076018	26.825201664062398	26.60784519946045	22.548229095716977
20	24.34372960240656	26.336204090286113	26.384198427788974	22.935867879518355
21	24.714833023565067	25.849657557545736	26.174715980828733	23.260793438060468
22	24.35206519892843	25.777540541702333	26.002112588179273	23.868281671189965
23	24.16315158405127	25.790836203618962	26.10396965405911	23.94204255827066
24	23.743240474060993	26.07043303953193	26.338789923303352	23.847536563103723
25	23.795164855013383	25.976507460265296	26.316134961270844	23.912192723450477
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	851.0
1	851.0
2	914.5
3	978.0
4	978.0
5	978.0
6	1276.0
7	1574.0
8	1574.0
9	1574.0
10	2050.0
11	2526.0
12	2526.0
13	2526.0
14	3811.5
15	5097.0
16	5097.0
17	5097.0
18	10441.0
19	15785.0
20	15785.0
21	15785.0
22	26444.5
23	37104.0
24	37104.0
25	37104.0
26	57094.5
27	77085.0
28	77085.0
29	77085.0
30	100226.5
31	123368.0
32	123368.0
33	123368.0
34	149790.5
35	176213.0
36	176213.0
37	176213.0
38	204739.5
39	233266.0
40	233266.0
41	233266.0
42	258082.5
43	282899.0
44	282899.0
45	282899.0
46	302830.5
47	322762.0
48	322762.0
49	322762.0
50	326407.0
51	330052.0
52	330052.0
53	330052.0
54	306248.0
55	282444.0
56	282444.0
57	282444.0
58	248789.0
59	215134.0
60	215134.0
61	215134.0
62	179868.0
63	144602.0
64	144602.0
65	144602.0
66	113069.0
67	81536.0
68	81536.0
69	81536.0
70	60205.5
71	38875.0
72	38875.0
73	38875.0
74	27176.5
75	15478.0
76	15478.0
77	15478.0
78	10544.5
79	5611.0
80	5611.0
81	5611.0
82	3815.0
83	2019.0
84	2019.0
85	2019.0
86	1334.0
87	649.0
88	649.0
89	649.0
90	411.5
91	174.0
92	174.0
93	174.0
94	102.0
95	30.0
96	30.0
97	30.0
98	17.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09978648779942208
2	0.0
3	0.04110819342636166
4	0.0580105470686728
5	0.0683606302866806
6	0.003004862869744203
7	1.252026195726751E-4
8	0.002086710326211252
9	0.0
10	0.0
11	0.0015441656413963264
12	0.0
13	4.590762717664754E-4
14	0.06013899160140827
15	0.021993926838266595
16	1.6693682609690016E-4
17	0.0
18	0.0020449761196870268
19	0.002170178739259702
20	0.0
21	0.009974475359289784
22	1.252026195726751E-4
23	5.425446848149255E-4
24	0.002211912945783927
25	0.1827123561630572
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2396116.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.31773097925577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.68898260038191	24.25470822265806
2	14.933474952333267	11.743007015225878
3	7.5494451838248375	8.904811643407893
4	4.379979672028225	6.888434497576587
5	2.8929390702079134	5.687190005090653
6	1.9524314596602668	4.605910493181482
7	1.339901454143374	3.6877319438905958
8	1.0257162684704215	3.2263069043812904
9	0.7812622323937525	2.764571244975932
>10	3.2986163881115216	21.023351906245267
>50	0.11102604799367768	2.9982535602912748
>100	0.043761323146385665	2.8612091337197016
>500	0.0012852246805424297	0.3705267569762208
>1k	0.001178122623830465	0.9839866723790001
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4872	0.20332905418602437	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3341	0.13943398399743587	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3335	0.13918357875829052	No Hit
GGTATCAACGCAGAGTACATGGGGG	2404	0.10032903248423698	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.173420652422504E-5	0.0
2	0.0	0.0	0.0	4.173420652422504E-5	0.0
3	0.0	0.0	0.0	4.173420652422504E-5	0.0
4	0.0	0.0	0.0	4.173420652422504E-5	0.0
5	0.0	4.173420652422504E-5	0.0	4.173420652422504E-5	4.173420652422504E-5
6	0.0	4.173420652422504E-5	0.0	4.173420652422504E-5	4.173420652422504E-5
7	0.0	4.173420652422504E-5	0.0	4.173420652422504E-5	4.173420652422504E-5
8	0.0	4.173420652422504E-5	0.0	4.173420652422504E-5	4.173420652422504E-5
9	0.0	4.173420652422504E-5	0.0	4.173420652422504E-5	4.173420652422504E-5
10	0.0	4.173420652422504E-5	0.0	4.173420652422504E-5	4.173420652422504E-5
11	0.0	4.173420652422504E-5	0.0	4.173420652422504E-5	4.173420652422504E-5
12	0.0	8.346841304845008E-5	0.0	4.173420652422504E-5	4.173420652422504E-5
13	0.0	8.346841304845008E-5	0.0	4.173420652422504E-5	4.173420652422504E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGA	65	5.4904885E-5	13.14473	18
CGTGCGC	100	1.4433681E-7	12.3502035	10
GCACCGT	100	1.9484596E-6	11.391386	6
CCATGCG	85	5.324521E-5	11.176655	9
GGTATCA	1785	0.0	11.139018	1
TCGAGTG	70	0.0014917077	10.857322	13
GTATCAA	3390	0.0	10.355369	1
CTAGATC	75	0.0026357407	10.139218	3
CCTACGT	75	0.0026357407	10.139218	2
AGGGCGC	150	2.4458132E-8	10.133501	10
CGTCCCA	95	1.6443928E-4	10.000165	10
GCGTGCG	115	1.0266811E-5	9.913206	9
TTATACT	290	0.0	9.833293	4
AGGACTG	470	0.0	9.701882	5
CCCTTAG	140	1.0387757E-6	9.513468	1
ATTATAC	250	0.0	9.505517	3
CCTATTC	170	1.6500053E-8	9.505516	3
TAGACAG	300	0.0	9.49976	5
TAATCCC	275	0.0	9.327037	5
CTGCGTT	370	0.0	9.243396	11
>>END_MODULE