FastQCFastQC Report
Mon 15 Aug 2016
SRR2921189_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921189_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091677
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16590.15196802717287258No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAA350.00216820916.2873251
GTATAGG902.2810127E-914.7792391
CTAATAC802.0048901E-613.0584053
CGTGCGC853.9520837E-612.29082710
TTACACT1405.2750693E-1112.2104574
GTTCCGG550.003065166312.0921061
GGTATCA7850.011.8610461
GCCATAC658.013407E-411.6934641
GAATTAT907.4465443E-611.6122591
GTCTAGA1104.956055E-711.2283841
GTATTAG951.3575651E-511.0010881
GTGTTAT700.001489555510.8582171
ACTATAC700.001494689810.8537393
TATAGGA1158.8046727E-710.7392082
AAGACTG1605.456968E-1010.683665
GCGTGCG909.547334E-510.5512799
GTATCAA17550.010.5024631
GTATTAA1659.258656E-1010.3646611
TTTATAC1201.5277928E-610.2884413
ATGGGTA1951.4551915E-1110.2270945
Produced by FastQC (version 0.11.5)