##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921189_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1091677
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.621162669910607	31.0	27.0	31.0	7.0	34.0
2	26.382020506065437	31.0	26.0	31.0	7.0	34.0
3	26.220794245917062	31.0	26.0	31.0	7.0	34.0
4	30.04600811412167	35.0	30.0	37.0	7.0	37.0
5	29.834361262534614	35.0	30.0	37.0	2.0	37.0
6	30.08041847542817	35.0	30.0	37.0	2.0	37.0
7	30.042271660940003	35.0	30.0	37.0	2.0	37.0
8	29.599605927394276	35.0	30.0	37.0	2.0	37.0
9	31.200930311804683	37.0	30.0	39.0	2.0	39.0
10	30.59530612076649	37.0	30.0	39.0	2.0	39.0
11	30.996705069356594	37.0	30.0	39.0	2.0	39.0
12	30.70223426892753	37.0	30.0	39.0	2.0	39.0
13	30.70397471046839	37.0	30.0	39.0	2.0	39.0
14	31.775210982735736	38.0	31.0	40.0	2.0	41.0
15	31.41976427093362	38.0	30.0	40.0	2.0	41.0
16	31.342484086410174	38.0	30.0	40.0	2.0	41.0
17	31.655288148417526	38.0	31.0	40.0	2.0	41.0
18	31.70742078471929	38.0	31.0	40.0	2.0	41.0
19	31.43508656864622	38.0	30.0	40.0	2.0	41.0
20	31.296072006646654	38.0	30.0	40.0	2.0	41.0
21	31.24568714006066	38.0	30.0	40.0	2.0	41.0
22	30.49993908454607	38.0	27.0	40.0	2.0	41.0
23	30.59845540393358	38.0	29.0	40.0	2.0	41.0
24	30.2304216357036	37.0	27.0	40.0	2.0	41.0
25	29.74273434358331	37.0	27.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	106799.0
3	2.0
4	891.0
5	4696.0
6	5450.0
7	4820.0
8	4116.0
9	3760.0
10	3542.0
11	3311.0
12	3190.0
13	3035.0
14	3186.0
15	3086.0
16	3306.0
17	3591.0
18	4155.0
19	5083.0
20	6267.0
21	7919.0
22	9565.0
23	11218.0
24	12706.0
25	14135.0
26	15821.0
27	17655.0
28	19726.0
29	23548.0
30	27272.0
31	32389.0
32	40428.0
33	51009.0
34	65291.0
35	84219.0
36	111798.0
37	149340.0
38	225352.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.069113647526265	18.066093407519812	12.593466165248179	25.271326779705745
2	16.093640592865235	19.90783680442266	39.00832914798535	24.990193454726757
3	19.090338569223228	24.000531376402307	29.590794361913137	27.318335692461325
4	13.647715425390597	16.2142774690391	35.07372166612615	35.06428543944416
5	13.853131919626293	36.86222631833052	34.22606914381463	15.058572618228558
6	33.06533316966911	34.46264700008977	17.478880903133376	14.99313892710774
7	28.797733314523676	31.94099142756115	20.80295848990233	18.45831676801284
8	26.2073640481654	34.01450091833043	21.009110021023126	18.76902501248105
9	27.049268182305376	16.172726748058118	19.200126053845878	37.57787901579063
10	15.963485963714977	28.506139872759668	32.60641148369189	22.92396267983346
11	35.09429295173355	23.249614079126378	22.05909971645695	19.596993252683124
12	23.514403865215805	25.226231286334876	28.928655840788874	22.33070900766044
13	28.595207733292295	21.772237246150578	25.24276469596699	24.38979032459014
14	22.706309389876097	21.417093290432756	25.93878605535787	29.937811264333273
15	24.64380561171628	28.148540365643743	23.16791085452758	24.039743168112395
16	23.996316421247467	27.425435019654916	25.04952672427222	23.528721834825397
17	23.092424314625358	27.3028184760655	25.677401972823276	23.927355236485866
18	22.900554232609107	27.16720159718678	26.722184605545625	23.210059564658486
19	24.147816287826732	26.5583743323347	26.965340955940963	22.328468423897608
20	24.843379807859687	26.056396095626727	26.196204468370876	22.90401962814271
21	25.27052062736963	25.798642861462284	25.8243892784956	23.10644723267248
22	24.092970677337114	25.01014553692563	27.166220995902396	23.73066278983486
23	24.336895725587382	25.60579755289763	26.245379044338268	23.811927677176715
24	24.05654370639781	25.7282109303785	26.432991768548252	23.78225359467544
25	23.728370469512004	25.537343905622617	26.963387894642853	23.770897730222522
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	695.0
1	695.0
2	736.0
3	777.0
4	777.0
5	777.0
6	992.0
7	1207.0
8	1207.0
9	1207.0
10	1544.5
11	1882.0
12	1882.0
13	1882.0
14	2768.5
15	3655.0
16	3655.0
17	3655.0
18	6287.0
19	8919.0
20	8919.0
21	8919.0
22	13987.5
23	19056.0
24	19056.0
25	19056.0
26	28215.0
27	37374.0
28	37374.0
29	37374.0
30	47863.0
31	58352.0
32	58352.0
33	58352.0
34	69037.5
35	79723.0
36	79723.0
37	79723.0
38	92309.5
39	104896.0
40	104896.0
41	104896.0
42	115680.5
43	126465.0
44	126465.0
45	126465.0
46	136047.5
47	145630.0
48	145630.0
49	145630.0
50	147045.0
51	148460.0
52	148460.0
53	148460.0
54	137967.0
55	127474.0
56	127474.0
57	127474.0
58	112469.0
59	97464.0
60	97464.0
61	97464.0
62	80971.0
63	64478.0
64	64478.0
65	64478.0
66	50368.0
67	36258.0
68	36258.0
69	36258.0
70	26750.0
71	17242.0
72	17242.0
73	17242.0
74	12218.5
75	7195.0
76	7195.0
77	7195.0
78	5024.5
79	2854.0
80	2854.0
81	2854.0
82	1994.5
83	1135.0
84	1135.0
85	1135.0
86	751.0
87	367.0
88	367.0
89	367.0
90	233.5
91	100.0
92	100.0
93	100.0
94	58.5
95	17.0
96	17.0
97	17.0
98	9.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034167615512647055
2	0.05212164403939993
3	0.015755576054089258
4	0.012732703904176787
5	0.011541875481483992
6	0.0010076240499708247
7	0.0035724852680783787
8	0.0020152480999416494
9	0.007328174908878725
10	0.003847291827161331
11	0.012091488599649897
12	0.008702207704293486
13	0.007786185840683645
14	0.014747952004118435
15	0.0062289486725469155
16	0.03178595866726147
17	0.04112938167608184
18	0.023633364081133888
19	0.01712960884950402
20	0.016763200104060086
21	0.024091375012938808
22	0.00586253992710298
23	0.016671597917699097
24	0.012091488599649897
25	0.4868656205086303
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1091677.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.232028715149134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.78342414212807	47.016048319182616
2	12.109892311919623	14.830265475616521
3	4.792326933972841	8.803317013002228
4	2.3500187971128854	5.755856738638256
5	1.2651690661132022	3.873443429288101
6	0.7549267290590458	2.7735417090946264
7	0.47365156934873465	2.030185254673602
8	0.3165558250573544	1.550668429588772
9	0.22139456443242417	1.220079449403372
>10	0.8890364939080545	8.917549546203336
>50	0.029333409224381053	1.2182941370553801
>100	0.013196061481670861	1.4207077879385033
>500	9.206539214494925E-4	0.43416996247839834
>1k	1.5344232024158208E-4	0.1558727478363261
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1659	0.15196802717287258	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCTAA	35	0.002168209	16.287325	1
GTATAGG	90	2.2810127E-9	14.779239	1
CTAATAC	80	2.0048901E-6	13.058405	3
CGTGCGC	85	3.9520837E-6	12.290827	10
TTACACT	140	5.2750693E-11	12.210457	4
GTTCCGG	55	0.0030651663	12.092106	1
GGTATCA	785	0.0	11.861046	1
GCCATAC	65	8.013407E-4	11.693464	1
GAATTAT	90	7.4465443E-6	11.612259	1
GTCTAGA	110	4.956055E-7	11.228384	1
GTATTAG	95	1.3575651E-5	11.001088	1
GTGTTAT	70	0.0014895555	10.858217	1
ACTATAC	70	0.0014946898	10.853739	3
TATAGGA	115	8.8046727E-7	10.739208	2
AAGACTG	160	5.456968E-10	10.68366	5
GCGTGCG	90	9.547334E-5	10.551279	9
GTATCAA	1755	0.0	10.502463	1
GTATTAA	165	9.258656E-10	10.364661	1
TTTATAC	120	1.5277928E-6	10.288441	3
ATGGGTA	195	1.4551915E-11	10.227094	5
>>END_MODULE