FastQCFastQC Report
Mon 15 Aug 2016
SRR2921189_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921189_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091677
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27970.2562113152516724No Hit
GGTATCAACGCAGAGTACTTTTTTT18820.172395314731372No Hit
TATCAACGCAGAGTACTTTTTTTTT18550.16992205569962546No Hit
GGTATCAACGCAGAGTACATGGGGG13720.12567819968727015No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12490.1144111307648691No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG550.003067825712.0907055
ACCGTCC550.00307098712.0890438
ACCGTCT951.0407566E-611.9981488
TACACCG907.4561503E-611.6109145
CTTATAC2400.011.4968921
TATACAC2400.011.4863443
GGTATCA8800.011.4608521
AAGACCG752.0730602E-411.3998075
GATCTCG1701.0913936E-1111.18705119
GTCTAGA1201.2465716E-711.1004481
GATCTAA600.00580667611.1004481
CAATACT600.005846487411.0902634
TATGCGA700.001489854510.85795512
CCGTATG700.001489854510.8579559
ACCGTAT803.7741126E-410.685858
TAAAACG909.500985E-510.55634412
GTATCAA18850.010.5494211
GTACCGT909.589683E-510.5466756
GCCTTAG1105.9285267E-610.3796391
CCCTTAC750.002612826910.1489791
Produced by FastQC (version 0.11.5)