##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921189_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1091677
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.164105316865705	31.0	30.0	33.0	25.0	34.0
2	29.462571804663835	31.0	30.0	34.0	25.0	34.0
3	29.616151114294794	31.0	30.0	34.0	25.0	34.0
4	33.26514069637814	35.0	35.0	37.0	28.0	37.0
5	32.71670833039443	35.0	33.0	37.0	26.0	37.0
6	32.661707629637704	35.0	33.0	37.0	25.0	37.0
7	32.512881557457014	35.0	33.0	37.0	24.0	37.0
8	32.452903193893434	36.0	33.0	37.0	23.0	37.0
9	33.788819403541524	38.0	34.0	39.0	23.0	39.0
10	33.66012749192298	38.0	34.0	39.0	22.0	39.0
11	33.672960042210285	38.0	34.0	39.0	22.0	39.0
12	33.53366426149859	38.0	34.0	39.0	19.0	39.0
13	33.498085972315984	38.0	34.0	39.0	19.0	39.0
14	34.42687168457337	39.0	34.0	41.0	17.0	41.0
15	34.41558263112624	39.0	34.0	41.0	17.0	41.0
16	34.38925799480982	39.0	34.0	41.0	17.0	41.0
17	34.36279961930131	39.0	34.0	41.0	17.0	41.0
18	34.31393992911823	39.0	34.0	41.0	17.0	41.0
19	34.3498186734721	39.0	34.0	41.0	18.0	41.0
20	34.28663698145147	39.0	34.0	41.0	18.0	41.0
21	34.22095454974319	39.0	34.0	41.0	16.0	41.0
22	34.117827892316136	39.0	34.0	41.0	11.0	41.0
23	33.992094731317046	39.0	34.0	41.0	10.0	41.0
24	33.90575875464996	39.0	34.0	41.0	2.0	41.0
25	33.76050699978107	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	44780.0
3	1.0
4	748.0
5	6053.0
6	6580.0
7	4904.0
8	3899.0
9	3335.0
10	2867.0
11	2630.0
12	2412.0
13	2319.0
14	2204.0
15	2228.0
16	2209.0
17	2289.0
18	2519.0
19	2949.0
20	3850.0
21	4883.0
22	6009.0
23	7265.0
24	8604.0
25	9736.0
26	10861.0
27	12411.0
28	13908.0
29	17031.0
30	21169.0
31	27323.0
32	34332.0
33	43707.0
34	58257.0
35	76525.0
36	103182.0
37	157603.0
38	380095.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.5877041123223	19.177297134725137	9.310584498466865	31.924414254485704
2	14.58206044461869	21.064563969012813	35.14885813294592	29.20451745342258
3	17.730693381425763	24.313069997947274	28.048389343421015	29.907847277205946
4	13.314110313614464	16.858026145384322	33.20993572264592	36.6179278183553
5	14.50232283565617	37.664587664587664	32.428032428032424	15.405057071723737
6	33.48145990322764	35.284719970246435	16.5528006639588	14.681019462567125
7	29.172365086009872	32.34610603685889	19.813003296762687	18.668525580368552
8	27.55799264780648	34.93006438894624	18.618747635453587	18.89319532779369
9	27.105819761706073	16.047603824208075	18.807852505823607	38.03872390826224
10	16.842985608380502	29.001893417192083	30.79821229173098	23.35690868269644
11	35.32641105854009	23.034777789482845	21.652551882709123	19.98625926926794
12	23.65507379930144	25.299974259785635	28.359029273310693	22.685922667602227
13	28.770428507437668	21.333677758816055	25.099823848350194	24.796069885396086
14	22.798666908646943	21.241285501898265	25.531944146552053	30.42810344290274
15	24.698696934102724	28.165262693027287	22.666859686318634	24.469180686551358
16	24.609678435855724	27.300249893740197	24.221738556918613	23.86833311348547
17	23.224635125591178	27.32603141771788	25.51917829174747	23.930155164943475
18	23.242320256348716	26.970937252092725	26.556880577627386	23.229861913931174
19	24.311061400109743	26.387198012537066	26.5289110084524	22.772829578900794
20	24.656194094040636	25.923693546717573	26.177156796378416	23.242955562863372
21	24.972813913413116	25.57553426271425	25.933648542303565	23.51800328156907
22	24.763002243337546	25.153593965980782	25.693039241460614	24.39036454922106
23	24.457618983060783	25.31328206011351	26.029433857180024	24.199665099645678
24	24.162326806366654	25.74785297148746	26.05464330699645	24.035176915149435
25	24.27483998256441	25.486888893987015	26.01527908417261	24.222992039275965
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	549.0
1	549.0
2	563.5
3	578.0
4	578.0
5	578.0
6	756.5
7	935.0
8	935.0
9	935.0
10	1229.5
11	1524.0
12	1524.0
13	1524.0
14	2077.5
15	2631.0
16	2631.0
17	2631.0
18	4892.5
19	7154.0
20	7154.0
21	7154.0
22	11899.0
23	16644.0
24	16644.0
25	16644.0
26	25509.0
27	34374.0
28	34374.0
29	34374.0
30	43985.0
31	53596.0
32	53596.0
33	53596.0
34	63927.5
35	74259.0
36	74259.0
37	74259.0
38	86369.0
39	98479.0
40	98479.0
41	98479.0
42	109707.0
43	120935.0
44	120935.0
45	120935.0
46	132121.5
47	143308.0
48	143308.0
49	143308.0
50	148288.5
51	153269.0
52	153269.0
53	153269.0
54	143980.5
55	134692.0
56	134692.0
57	134692.0
58	119570.5
59	104449.0
60	104449.0
61	104449.0
62	87931.5
63	71414.0
64	71414.0
65	71414.0
66	55926.0
67	40438.0
68	40438.0
69	40438.0
70	29858.0
71	19278.0
72	19278.0
73	19278.0
74	13661.5
75	8045.0
76	8045.0
77	8045.0
78	5652.0
79	3259.0
80	3259.0
81	3259.0
82	2273.5
83	1288.0
84	1288.0
85	1288.0
86	864.0
87	440.0
88	440.0
89	440.0
90	279.5
91	119.0
92	119.0
93	119.0
94	69.0
95	19.0
96	19.0
97	19.0
98	10.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10085400718344345
2	0.0
3	0.04076297293063791
4	0.05715976428925406
5	0.07044208131159674
6	0.003938894013522314
7	0.0
8	0.0027480655908295216
9	0.0
10	0.0
11	0.00293126996355149
12	0.0
13	3.6640874544393627E-4
14	0.062197884539108184
15	0.022992148776607
16	0.0011908284226927928
17	0.0
18	0.0035724852680783787
19	0.0031144743362734578
20	0.0
21	0.011541875481483992
22	0.0
23	8.244196772488567E-4
24	0.004763313690771171
25	0.1788074677766409
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1091677.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.374839148524444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.20457838662067	33.675900870323225
2	14.814833867897322	14.62960078479085
3	6.626168734098256	9.814980462512498
4	3.499025133277906	6.910552125289637
5	2.035107299792057	5.024154778861037
6	1.259192003607391	3.7303441581133883
7	0.8150235663018492	2.8169160241887394
8	0.5713672591056058	2.256893321045806
9	0.40967714644618475	1.8204968887746178
>10	1.670763029111037	13.54969393009424
>50	0.06540157877073069	2.133540131046876
>100	0.026255106564820288	2.2119629041231
>500	0.001675856832544533	0.583261449808655
>1k	9.310315736358516E-4	0.8417021710274648
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2797	0.2562113152516724	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1882	0.172395314731372	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1855	0.16992205569962546	No Hit
GGTATCAACGCAGAGTACATGGGGG	1372	0.12567819968727015	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1249	0.1144111307648691	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGACCG	55	0.0030678257	12.090705	5
ACCGTCC	55	0.003070987	12.089043	8
ACCGTCT	95	1.0407566E-6	11.998148	8
TACACCG	90	7.4561503E-6	11.610914	5
CTTATAC	240	0.0	11.496892	1
TATACAC	240	0.0	11.486344	3
GGTATCA	880	0.0	11.460852	1
AAGACCG	75	2.0730602E-4	11.399807	5
GATCTCG	170	1.0913936E-11	11.187051	19
GTCTAGA	120	1.2465716E-7	11.100448	1
GATCTAA	60	0.005806676	11.100448	1
CAATACT	60	0.0058464874	11.090263	4
TATGCGA	70	0.0014898545	10.857955	12
CCGTATG	70	0.0014898545	10.857955	9
ACCGTAT	80	3.7741126E-4	10.68585	8
TAAAACG	90	9.500985E-5	10.556344	12
GTATCAA	1885	0.0	10.549421	1
GTACCGT	90	9.589683E-5	10.546675	6
GCCTTAG	110	5.9285267E-6	10.379639	1
CCCTTAC	75	0.0026128269	10.148979	1
>>END_MODULE