FastQCFastQC Report
Mon 15 Aug 2016
SRR2921188_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921188_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1968915
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23040.11701876414167192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA400.005292989314.2446279
GGTATCA12000.014.0136241
ACACCGA551.9672743E-413.8126226
ACGGTGC1001.9390245E-611.3957028
CTTACAC2100.011.3052613
CTAGGGT1303.2878233E-810.9568494
TTTTACG700.001492291110.8566342
GTACTAG1054.1020998E-59.953181
ACTATAC1356.4768574E-79.8481373
GTATCAA23850.09.8393641
GTCTAGA2054.1836756E-119.7324891
GTCTAGT1106.831169E-59.5007621
ACCCCGC1002.7661302E-49.4964188
AGCGCTG1502.6648013E-79.4959365
TAAGACC1752.6879206E-89.2246244
GTATATA2900.09.1731491
AGCCTCG1356.8514364E-69.1477192
CGTGCGC1151.11309906E-49.08514610
TCTATAC1151.11525704E-49.0835313
GTCTATA1054.489201E-49.0483461
Produced by FastQC (version 0.11.5)