##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921188_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1968915
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.908680669302637	30.0	26.0	31.0	2.0	34.0
2	25.550848055908965	31.0	25.0	31.0	2.0	34.0
3	25.33295850760444	30.0	25.0	31.0	2.0	34.0
4	29.061552174674883	35.0	28.0	37.0	2.0	37.0
5	28.673933105288953	35.0	28.0	37.0	2.0	37.0
6	29.118190475464914	35.0	29.0	37.0	2.0	37.0
7	28.934968751825245	35.0	28.0	37.0	2.0	37.0
8	28.629842324325836	35.0	28.0	37.0	2.0	37.0
9	30.183127255366536	37.0	29.0	39.0	2.0	39.0
10	29.537669731806606	35.0	27.0	39.0	2.0	39.0
11	29.98694357044362	37.0	29.0	39.0	2.0	39.0
12	29.68809572785011	37.0	27.0	39.0	2.0	39.0
13	29.671511466975467	37.0	27.0	39.0	2.0	39.0
14	30.677565054865244	37.0	28.0	40.0	2.0	41.0
15	30.353168623328077	37.0	27.0	40.0	2.0	41.0
16	30.261100149066873	37.0	27.0	40.0	2.0	41.0
17	30.553321499404493	37.0	28.0	40.0	2.0	41.0
18	30.5877628033714	38.0	29.0	40.0	2.0	41.0
19	30.293274214478533	37.0	27.0	40.0	2.0	41.0
20	30.182392840727	37.0	27.0	40.0	2.0	41.0
21	30.109977322535507	37.0	27.0	40.0	2.0	41.0
22	29.397125320290616	37.0	25.0	40.0	2.0	41.0
23	29.46443752015704	37.0	26.0	40.0	2.0	41.0
24	29.099235873564883	37.0	25.0	40.0	2.0	41.0
25	28.627253081011624	37.0	22.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	236393.0
3	11.0
4	1809.0
5	9658.0
6	11166.0
7	9434.0
8	7870.0
9	7440.0
10	6877.0
11	6521.0
12	6221.0
13	5978.0
14	6014.0
15	6160.0
16	6490.0
17	6952.0
18	8144.0
19	10007.0
20	12438.0
21	15486.0
22	18494.0
23	21413.0
24	24353.0
25	27002.0
26	30373.0
27	33335.0
28	36712.0
29	42987.0
30	50457.0
31	59670.0
32	74079.0
33	92858.0
34	118632.0
35	151878.0
36	197329.0
37	257526.0
38	350748.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.82810261329388	17.975312003999505	12.203822290325792	25.992763092380823
2	15.953553960490376	20.449977767896844	38.34735437972432	25.24911389188846
3	19.51342663708255	23.83416030689456	29.36924471102659	27.2831683449963
4	13.728788850809945	16.085958520193607	34.45772043417109	35.727532194825365
5	14.103501837217546	36.63204550326148	33.572572886793466	15.691879772727505
6	33.233971642048026	34.18693982074264	17.719834567609034	14.8592539696003
7	28.699001748235766	31.56037914672686	20.542481316428642	19.198137788608737
8	26.797756090376023	33.79323830492893	20.60948201647125	18.799523588223792
9	27.02062028993457	15.953918881098991	19.240863292104944	37.78459753686149
10	16.1811224386141	28.03876972211274	32.74277042099575	23.037337418277414
11	35.30059846416885	22.792788765818766	22.168368461314184	19.7382443086982
12	23.513492372286464	24.89676148525302	29.261782397084023	22.32796374537649
13	28.49833624805269	21.8269728451098	25.091086038147182	24.583604868690326
14	22.876164274451796	21.058687153562314	25.667171930623063	30.397976641362828
15	24.440621457218437	28.15142483309562	23.19584801238526	24.21210569730068
16	24.203496435992662	26.964624115348855	24.791417931300774	24.040461517357706
17	23.33421402948977	27.167579542105745	25.75091202861585	23.747294399788633
18	23.240214722633	26.83250588140268	26.490213579626648	23.437065816337675
19	24.235501851843384	26.37156039965884	26.703525741156437	22.689412007341335
20	24.792492126384232	25.82586609773443	26.141877476379154	23.239764299502184
21	25.12988573306761	25.638557228210264	25.717705354589192	23.513851684132934
22	24.279466045775486	25.026678917889882	27.053311614332305	23.64054342200232
23	24.54838326236898	25.302119177443394	26.079526809489412	24.069970750698214
24	24.076764360173396	25.727113783186045	26.311314112951155	23.884807743689407
25	23.705575233838733	25.508668452426214	26.787924128523656	23.9978321852114
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	985.0
1	985.0
2	1075.5
3	1166.0
4	1166.0
5	1166.0
6	1603.0
7	2040.0
8	2040.0
9	2040.0
10	2534.0
11	3028.0
12	3028.0
13	3028.0
14	4553.0
15	6078.0
16	6078.0
17	6078.0
18	10342.0
19	14606.0
20	14606.0
21	14606.0
22	23052.0
23	31498.0
24	31498.0
25	31498.0
26	46855.5
27	62213.0
28	62213.0
29	62213.0
30	81753.0
31	101293.0
32	101293.0
33	101293.0
34	121354.5
35	141416.0
36	141416.0
37	141416.0
38	164719.0
39	188022.0
40	188022.0
41	188022.0
42	209069.0
43	230116.0
44	230116.0
45	230116.0
46	244834.0
47	259552.0
48	259552.0
49	259552.0
50	263130.5
51	266709.0
52	266709.0
53	266709.0
54	250377.0
55	234045.0
56	234045.0
57	234045.0
58	206920.0
59	179795.0
60	179795.0
61	179795.0
62	150991.0
63	122187.0
64	122187.0
65	122187.0
66	95764.0
67	69341.0
68	69341.0
69	69341.0
70	51066.0
71	32791.0
72	32791.0
73	32791.0
74	23184.5
75	13578.0
76	13578.0
77	13578.0
78	9526.5
79	5475.0
80	5475.0
81	5475.0
82	3762.0
83	2049.0
84	2049.0
85	2049.0
86	1377.5
87	706.0
88	706.0
89	706.0
90	450.0
91	194.0
92	194.0
93	194.0
94	109.5
95	25.0
96	25.0
97	25.0
98	16.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0340796834804956
2	0.052820969925060246
3	0.014779713700185127
4	0.010056300043424933
5	0.008989722766091984
6	7.110515182219649E-4
7	0.00370762577358596
8	0.0015236818247613532
9	0.006399463663997684
10	0.002996574255363995
11	0.01061498337917076
12	0.008786565189457137
13	0.008735775795298426
14	0.015592344006724516
15	0.006247095481521549
16	0.03174337134919486
17	0.03936178047300162
18	0.02143312433497637
19	0.017065236437327158
20	0.01599865915999421
21	0.023058384948055146
22	0.006704200028949954
23	0.01630339552494648
24	0.012240243992249537
25	0.4742713626540506
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1968915.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.835239116475535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.52389408130486	41.41370790955723
2	11.033324126235405	12.100299334233938
3	4.94305992609695	8.131616190437825
4	2.691757534415623	5.904126721730212
5	1.5931085568804355	4.367924432752098
6	1.0499214157230292	3.4543615130808494
7	0.6962959741940908	2.6727089368540575
8	0.5064375094000328	2.2216497540402504
9	0.3637062947078392	1.7949529474624515
>10	1.527368067145729	13.576790881277065
>50	0.05198041987192492	1.9418111361338741
>100	0.017432309476486878	1.613894629483169
>500	0.0013329435370290376	0.4831399548153534
>1k	3.8084101057972306E-4	0.323015658141623
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2304	0.11701876414167192	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.078939415871178E-5
2	0.0	0.0	0.0	0.0	5.078939415871178E-5
3	0.0	0.0	0.0	0.0	5.078939415871178E-5
4	0.0	0.0	0.0	0.0	5.078939415871178E-5
5	0.0	0.0	0.0	0.0	5.078939415871178E-5
6	0.0	0.0	0.0	0.0	5.078939415871178E-5
7	0.0	0.0	0.0	0.0	5.078939415871178E-5
8	0.0	0.0	0.0	0.0	5.078939415871178E-5
9	0.0	0.0	0.0	0.0	5.078939415871178E-5
10	0.0	0.0	0.0	0.0	5.078939415871178E-5
11	0.0	0.0	0.0	0.0	5.078939415871178E-5
12	0.0	0.0	0.0	0.0	5.078939415871178E-5
13	0.0	0.0	0.0	0.0	5.078939415871178E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGCTA	40	0.0052929893	14.244627	9
GGTATCA	1200	0.0	14.013624	1
ACACCGA	55	1.9672743E-4	13.812622	6
ACGGTGC	100	1.9390245E-6	11.395702	8
CTTACAC	210	0.0	11.305261	3
CTAGGGT	130	3.2878233E-8	10.956849	4
TTTTACG	70	0.0014922911	10.856634	2
GTACTAG	105	4.1020998E-5	9.95318	1
ACTATAC	135	6.4768574E-7	9.848137	3
GTATCAA	2385	0.0	9.839364	1
GTCTAGA	205	4.1836756E-11	9.732489	1
GTCTAGT	110	6.831169E-5	9.500762	1
ACCCCGC	100	2.7661302E-4	9.496418	8
AGCGCTG	150	2.6648013E-7	9.495936	5
TAAGACC	175	2.6879206E-8	9.224624	4
GTATATA	290	0.0	9.173149	1
AGCCTCG	135	6.8514364E-6	9.147719	2
CGTGCGC	115	1.11309906E-4	9.085146	10
TCTATAC	115	1.11525704E-4	9.083531	3
GTCTATA	105	4.489201E-4	9.048346	1
>>END_MODULE