FastQCFastQC Report
Mon 15 Aug 2016
SRR2921188_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921188_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1968915
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39800.20214178875167288No Hit
GGTATCAACGCAGAGTACTTTTTTT27540.13987399151309224No Hit
TATCAACGCAGAGTACTTTTTTTTT26410.1341347899731578No Hit
CTTATACACATCTCCGAGCCCACGA24660.12524664599538324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12700.014.1592911
TATACAC5000.012.1673393
CTTATAC4750.011.6176391
AGATCGT907.5231674E-611.6027776
GTCGGAT600.00583146411.0948219
TGCTACG600.00587689511.08325910
TTGCGCG600.00590809511.07537718
TACACCG1053.4684708E-610.8567945
AGGACCG909.520207E-510.5552175
TAATGGA2450.010.4757074
GTATCAA27400.010.3825511
GATCTAT750.00261493710.1487411
TTATACA6000.010.139452
GACCGCG750.00266392810.126067
GTATTAT951.6193067E-410.0152051
GGACCGC1054.1382384E-59.9452376
AACACCG1252.5713525E-69.8796835
ACACCGT1252.5939953E-69.8729086
AGGCCGA1356.5071436E-79.844786
AGTACTG2450.09.6935665
Produced by FastQC (version 0.11.5)