##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921188_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1968915
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.93589210301105	31.0	30.0	33.0	24.0	34.0
2	29.24549967875708	31.0	30.0	34.0	25.0	34.0
3	29.418144003169257	31.0	30.0	34.0	25.0	34.0
4	33.046841026656814	35.0	33.0	37.0	27.0	37.0
5	32.466078017588366	35.0	33.0	37.0	25.0	37.0
6	32.40191933120526	35.0	33.0	37.0	23.0	37.0
7	32.24445798828289	35.0	33.0	37.0	22.0	37.0
8	32.18006871805029	35.0	33.0	37.0	19.0	37.0
9	33.497176363631745	38.0	34.0	39.0	18.0	39.0
10	33.35458463163722	38.0	33.0	39.0	17.0	39.0
11	33.375708448561774	38.0	34.0	39.0	17.0	39.0
12	33.24018964759779	38.0	33.0	39.0	17.0	39.0
13	33.195090697160616	38.0	33.0	39.0	17.0	39.0
14	34.11560072425676	39.0	33.0	41.0	17.0	41.0
15	34.10106987858795	39.0	33.0	41.0	17.0	41.0
16	34.071837534885965	39.0	33.0	41.0	16.0	41.0
17	34.03725452850936	39.0	33.0	41.0	16.0	41.0
18	33.98910465916507	39.0	33.0	41.0	11.0	41.0
19	34.0176645512884	39.0	34.0	41.0	10.0	41.0
20	33.95382685387637	39.0	34.0	41.0	10.0	41.0
21	33.88053369495382	39.0	34.0	41.0	10.0	41.0
22	33.77608429007854	39.0	34.0	41.0	2.0	41.0
23	33.65202611590647	39.0	34.0	41.0	2.0	41.0
24	33.56053714863262	39.0	34.0	41.0	2.0	41.0
25	33.42618244058276	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	89904.0
3	6.0
4	1504.0
5	12081.0
6	13206.0
7	9786.0
8	7590.0
9	6335.0
10	5525.0
11	5072.0
12	4703.0
13	4300.0
14	4198.0
15	4109.0
16	4241.0
17	4374.0
18	4913.0
19	5628.0
20	6954.0
21	9091.0
22	11333.0
23	13654.0
24	16092.0
25	18121.0
26	20188.0
27	22634.0
28	25610.0
29	30754.0
30	38639.0
31	48786.0
32	61767.0
33	79587.0
34	104021.0
35	136041.0
36	185432.0
37	280769.0
38	671967.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.528113879003556	19.072394509405186	9.171581087951195	32.22791052364006
2	14.58168585236031	21.000906590685734	34.858183314160335	29.55922424279362
3	17.64434785657413	23.890355942787778	28.15881376455064	30.30648243608746
4	13.369813616177487	16.713308781642233	32.94369762290371	36.97317997927657
5	14.677864400284415	37.37975472938048	32.53980476137473	15.402576108960373
6	33.60549200107475	35.09008155617533	16.55864126203064	14.745785180719281
7	29.18816596177591	32.01934314373321	19.867507287200787	18.9249836072901
8	27.37284149769255	34.939827573771794	18.854788753998545	18.832542174537114
9	26.872263700543453	15.942861496266952	18.88973538524049	38.29513941794911
10	16.738026091646834	28.662617717656403	31.271172925472317	23.328183265224446
11	35.45885392038301	22.638737009555317	21.988713213950675	19.913695856110998
12	23.59550390597026	25.052148169366895	28.624126356271418	22.72822156839143
13	28.556880579708082	21.519077351127788	25.123257043568003	24.80078502559612
14	22.803519085428075	20.831097315559024	25.601387144117442	30.763996454895455
15	24.53668307705125	28.114488975950348	22.694693185414653	24.654134761583748
16	24.60033877558276	26.93067798288853	24.115793189442595	24.35319005208611
17	23.26488529069679	27.223105404568056	25.717536276785395	23.79447302794976
18	23.29743424086727	26.614900459760303	26.516670628366175	23.57099467100625
19	24.335107125932844	26.237485721367438	26.340794707166772	23.086612445532953
20	24.751533866970053	25.781205558490107	25.999045142415994	23.46821543212385
21	24.95692816552587	25.419340879031125	25.81638395696067	23.807346998482327
22	24.735926536354704	25.40199556597329	25.682156978766958	24.179920918905054
23	24.368807625556606	25.23718124207343	25.87242419825147	24.521586934118496
24	24.035529333845307	25.81016754941458	26.043095164085468	24.11120795265464
25	24.05510292751473	25.56352954224704	26.021923545374904	24.359443984863333
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	904.0
1	904.0
2	993.5
3	1083.0
4	1083.0
5	1083.0
6	1304.0
7	1525.0
8	1525.0
9	1525.0
10	1942.0
11	2359.0
12	2359.0
13	2359.0
14	3354.5
15	4350.0
16	4350.0
17	4350.0
18	8068.0
19	11786.0
20	11786.0
21	11786.0
22	20016.5
23	28247.0
24	28247.0
25	28247.0
26	42834.5
27	57422.0
28	57422.0
29	57422.0
30	76082.5
31	94743.0
32	94743.0
33	94743.0
34	113838.5
35	132934.0
36	132934.0
37	132934.0
38	156610.0
39	180286.0
40	180286.0
41	180286.0
42	201463.0
43	222640.0
44	222640.0
45	222640.0
46	240155.0
47	257670.0
48	257670.0
49	257670.0
50	265728.5
51	273787.0
52	273787.0
53	273787.0
54	259562.5
55	245338.0
56	245338.0
57	245338.0
58	217569.5
59	189801.0
60	189801.0
61	189801.0
62	161142.0
63	132483.0
64	132483.0
65	132483.0
66	103879.5
67	75276.0
68	75276.0
69	75276.0
70	54846.0
71	34416.0
72	34416.0
73	34416.0
74	24102.5
75	13789.0
76	13789.0
77	13789.0
78	9594.0
79	5399.0
80	5399.0
81	5399.0
82	3647.0
83	1895.0
84	1895.0
85	1895.0
86	1250.5
87	606.0
88	606.0
89	606.0
90	381.0
91	156.0
92	156.0
93	156.0
94	85.5
95	15.0
96	15.0
97	15.0
98	10.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09726168981393306
2	0.0
3	0.04438993049471409
4	0.05556359720963069
5	0.06978462757406999
6	0.004469466685966637
7	0.0026410484962530126
8	0.00370762577358596
9	7.618409123806766E-4
10	4.5710454742840594E-4
11	0.0032505212261575538
12	3.0473636495227067E-4
13	0.00314894243784013
14	0.05744280479350301
15	0.022652069794785453
16	0.0030473636495227064
17	0.0012697348539677945
18	0.003199731831998842
19	0.0032505212261575538
20	0.0017776287955549122
21	0.0056376227516170075
22	0.002539469707935589
23	0.00314894243784013
24	0.003199731831998842
25	0.17049999619079542
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1968915.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.207032802156675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.23674477948173	25.827689044523883
2	13.826420274727635	11.118386670447563
3	7.109177045229383	8.575167439616314
4	4.092289374980048	6.5815525254296015
5	2.6945897406875883	5.417072904609036
6	1.8004184507382817	4.343369022386531
7	1.32384098963999	3.7259402666706256
8	0.9131266910386399	2.9371291855292303
9	0.6965148244747408	2.520431495535987
>10	3.1421783507706613	21.005344186462253
>50	0.11478357372390934	3.0807205063365375
>100	0.04596949608327733	3.10597349755662
>500	0.0026733734486074413	0.7242159851643728
>1k	0.0012730349755273526	1.0370072697314432
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3980	0.20214178875167288	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2754	0.13987399151309224	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2641	0.1341347899731578	No Hit
CTTATACACATCTCCGAGCCCACGA	2466	0.12524664599538324	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1270	0.0	14.159291	1
TATACAC	500	0.0	12.167339	3
CTTATAC	475	0.0	11.617639	1
AGATCGT	90	7.5231674E-6	11.602777	6
GTCGGAT	60	0.005831464	11.09482	19
TGCTACG	60	0.005876895	11.083259	10
TTGCGCG	60	0.005908095	11.075377	18
TACACCG	105	3.4684708E-6	10.856794	5
AGGACCG	90	9.520207E-5	10.555217	5
TAATGGA	245	0.0	10.475707	4
GTATCAA	2740	0.0	10.382551	1
GATCTAT	75	0.002614937	10.148741	1
TTATACA	600	0.0	10.13945	2
GACCGCG	75	0.002663928	10.12606	7
GTATTAT	95	1.6193067E-4	10.015205	1
GGACCGC	105	4.1382384E-5	9.945237	6
AACACCG	125	2.5713525E-6	9.879683	5
ACACCGT	125	2.5939953E-6	9.872908	6
AGGCCGA	135	6.5071436E-7	9.84478	6
AGTACTG	245	0.0	9.693566	5
>>END_MODULE