FastQCFastQC Report
Mon 15 Aug 2016
SRR2921187_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921187_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1806999
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20350.11261766055210877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8800.013.6021741
GTGTAGG1900.012.4999091
ACCGTAT853.9629867E-612.2889248
GTATAAA2000.011.8749131
CGGAAAT1001.9372128E-611.39644510
GTATCAA21650.011.1454161
CAATACA2000.010.9200824
CCGTATG1053.4842178E-610.8525569
TAGACCG803.7860664E-410.6829855
AGACCGT750.002657224410.1290536
CTAGACA1606.217306E-910.0892074
TATGCGA856.606246E-410.05680112
TCTATAC856.619708E-410.0545743
CCCTTAC1054.1055406E-59.9523091
TCTTATA2054.1836756E-119.7305582
CTTACAC2450.09.6897833
ATGCGCC800.00452354559.49808911
ACCCCGT1304.2550237E-69.49703910
AGGACCG1804.2109605E-99.4959875
GTTAATC800.0045318169.4959873
Produced by FastQC (version 0.11.5)