##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921187_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1806999
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.132443349442916	31.0	26.0	31.0	2.0	34.0
2	25.854914695580906	31.0	26.0	31.0	2.0	34.0
3	25.46987574425885	30.0	25.0	31.0	2.0	34.0
4	29.143577279234798	35.0	28.0	37.0	2.0	37.0
5	28.936338647669423	35.0	28.0	37.0	2.0	37.0
6	29.32264212653134	35.0	29.0	37.0	2.0	37.0
7	29.182859536723594	35.0	29.0	37.0	2.0	37.0
8	28.85834524534878	35.0	28.0	37.0	2.0	37.0
9	30.34527855300418	37.0	30.0	39.0	2.0	39.0
10	29.660278727326357	35.0	27.0	39.0	2.0	39.0
11	30.198062090792526	37.0	29.0	39.0	2.0	39.0
12	29.896236245841862	37.0	28.0	39.0	2.0	39.0
13	29.920650205119095	37.0	28.0	39.0	2.0	39.0
14	30.957570535456853	37.0	29.0	40.0	2.0	41.0
15	30.626348990785274	37.0	28.0	40.0	2.0	41.0
16	30.56114806925737	37.0	27.0	40.0	2.0	41.0
17	30.84252343249775	38.0	29.0	40.0	2.0	41.0
18	30.89237846838875	38.0	30.0	40.0	2.0	41.0
19	30.58547071691794	38.0	28.0	40.0	2.0	41.0
20	30.452828142129576	37.0	27.0	40.0	2.0	41.0
21	30.411182850682263	38.0	28.0	40.0	2.0	41.0
22	29.678883054168818	37.0	26.0	40.0	2.0	41.0
23	29.764030306602272	37.0	27.0	40.0	2.0	41.0
24	29.388355499920035	37.0	26.0	40.0	2.0	41.0
25	28.904807916329784	37.0	24.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	204923.0
3	18.0
4	1459.0
5	8380.0
6	9964.0
7	8343.0
8	7051.0
9	6500.0
10	6182.0
11	5839.0
12	5472.0
13	5422.0
14	5357.0
15	5476.0
16	5885.0
17	6530.0
18	7340.0
19	9080.0
20	11131.0
21	14012.0
22	16893.0
23	19583.0
24	22477.0
25	25365.0
26	27981.0
27	30979.0
28	34480.0
29	40304.0
30	47292.0
31	55886.0
32	69275.0
33	87013.0
34	110891.0
35	141733.0
36	184613.0
37	238799.0
38	319071.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.71860887125793	17.57411628980668	12.410195203989332	25.297079634946062
2	15.983316325937377	19.224706366339863	39.125463643022975	25.666513664699785
3	19.698264506173523	23.38649330177756	28.58090890373201	28.33433328831691
4	14.302745052894211	15.468941462707283	34.12526137167412	36.103052112724384
5	14.519278344695675	36.759081847595205	33.273007218102705	15.44863258960642
6	34.72210923487552	33.77390097438443	16.69248312245744	14.811506668282618
7	30.260995168600886	31.481739746420722	19.411764705882355	18.845500379096038
8	27.452677529823504	33.902723020491834	19.6437781826312	19.000821267053468
9	27.154770219458985	16.445421933058757	18.514714167595514	37.885093679886744
10	16.543160956625975	27.92105301023723	31.45217257520197	24.08361345793482
11	36.15001621635383	22.86544632608265	21.36247258993435	19.62206486762917
12	24.366240212171995	24.263740594055022	28.093141823607677	23.276877370165305
13	28.883709809712194	21.239377875874037	24.855853472522778	25.02105884189099
14	23.150131978256937	20.59170759219521	25.052069006169685	31.20609142337817
15	24.91590448476208	27.652453906581197	22.543464569195194	24.888177039461524
16	24.77813889659288	27.09055409502647	24.076918861196962	24.05438814718369
17	23.87847254133167	27.088562883600314	24.98668506213453	24.046279512933484
18	23.932165637003017	26.753246609471947	25.69383978651339	23.620747967011646
19	24.885230584955938	26.067740246398614	26.123090820328716	22.92393834831673
20	25.214118954605784	25.64368524818094	25.59254245018282	23.549653347030453
21	25.701698401566276	25.25538572585453	25.319207940302707	23.723707932276493
22	24.735512107003863	24.607059208982516	26.436392294375743	24.221036389637874
23	24.85342230482736	25.077806700647194	25.682548661359444	24.386222333165993
24	24.68692819356488	25.296522974096376	25.74323075271174	24.273318079627003
25	24.419617810453246	25.145863579672678	26.202898421787484	24.231620188086588
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	911.0
1	911.0
2	981.0
3	1051.0
4	1051.0
5	1051.0
6	1352.0
7	1653.0
8	1653.0
9	1653.0
10	2098.0
11	2543.0
12	2543.0
13	2543.0
14	3513.5
15	4484.0
16	4484.0
17	4484.0
18	7608.0
19	10732.0
20	10732.0
21	10732.0
22	17084.5
23	23437.0
24	23437.0
25	23437.0
26	35640.0
27	47843.0
28	47843.0
29	47843.0
30	64122.5
31	80402.0
32	80402.0
33	80402.0
34	99994.0
35	119586.0
36	119586.0
37	119586.0
38	142618.0
39	165650.0
40	165650.0
41	165650.0
42	185629.0
43	205608.0
44	205608.0
45	205608.0
46	223926.5
47	242245.0
48	242245.0
49	242245.0
50	246390.5
51	250536.0
52	250536.0
53	250536.0
54	238084.0
55	225632.0
56	225632.0
57	225632.0
58	199588.0
59	173544.0
60	173544.0
61	173544.0
62	147053.5
63	120563.0
64	120563.0
65	120563.0
66	95248.0
67	69933.0
68	69933.0
69	69933.0
70	52987.0
71	36041.0
72	36041.0
73	36041.0
74	25606.5
75	15172.0
76	15172.0
77	15172.0
78	10674.0
79	6176.0
80	6176.0
81	6176.0
82	4166.0
83	2156.0
84	2156.0
85	2156.0
86	1502.5
87	849.0
88	849.0
89	849.0
90	528.5
91	208.0
92	208.0
93	208.0
94	120.0
95	32.0
96	32.0
97	32.0
98	22.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032152757140430074
2	0.051023824584296947
3	0.013890433807655676
4	0.010237969141100797
5	0.008467077181559037
6	6.640844848281598E-4
7	0.003818485787761919
8	0.0019369130807487993
9	0.0055893777473036785
10	0.003597124292819199
11	0.010016607646158077
12	0.009020480918915838
13	0.008688438676501757
14	0.014499177918748156
15	0.006917546716959999
16	0.03187605527175168
17	0.04189266291790975
18	0.023353637716456956
19	0.018428344453981435
20	0.016214729504554236
21	0.023132276221514234
22	0.006142781484660479
23	0.015384623898519035
24	0.012451584090527996
25	0.4743776836622488
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1806999.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.14052068798213
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.75874344519163	51.15767333993772
2	11.024691329367162	14.142588845797832
3	4.074221489279237	7.839680631616089
4	1.8601202780317152	4.772363327009132
5	0.9953993613759399	3.192271666556884
6	0.5955420668379005	2.2919026955147994
7	0.3971081615045725	1.7829506973845406
8	0.26249287932147725	1.3469143965253767
9	0.18269785859753057	1.054650220112245
>10	0.7947337781823682	8.607862225620073
>50	0.037700718919247096	1.6309036405223427
>100	0.015668688966293937	1.6443396248026754
>500	6.159610973924171E-4	0.2921903644953913
>1k	2.6398332745389296E-4	0.24370832410490623
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2035	0.11261766055210877	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6602112120703996E-4
2	0.0	0.0	0.0	0.0	1.6602112120703996E-4
3	0.0	0.0	0.0	0.0	1.6602112120703996E-4
4	0.0	0.0	0.0	0.0	1.6602112120703996E-4
5	0.0	0.0	0.0	0.0	1.6602112120703996E-4
6	0.0	5.5340373735679986E-5	0.0	0.0	2.2136149494271994E-4
7	0.0	5.5340373735679986E-5	0.0	0.0	2.7670186867839996E-4
8	0.0	5.5340373735679986E-5	0.0	0.0	2.7670186867839996E-4
9	0.0	5.5340373735679986E-5	0.0	0.0	2.7670186867839996E-4
10	0.0	5.5340373735679986E-5	0.0	0.0	2.7670186867839996E-4
11	0.0	5.5340373735679986E-5	0.0	0.0	2.7670186867839996E-4
12	0.0	1.1068074747135997E-4	0.0	0.0	7.194248585638398E-4
13	0.0	1.1068074747135997E-4	0.0	0.0	7.747652322995199E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	880	0.0	13.602174	1
GTGTAGG	190	0.0	12.499909	1
ACCGTAT	85	3.9629867E-6	12.288924	8
GTATAAA	200	0.0	11.874913	1
CGGAAAT	100	1.9372128E-6	11.396445	10
GTATCAA	2165	0.0	11.145416	1
CAATACA	200	0.0	10.920082	4
CCGTATG	105	3.4842178E-6	10.852556	9
TAGACCG	80	3.7860664E-4	10.682985	5
AGACCGT	75	0.0026572244	10.129053	6
CTAGACA	160	6.217306E-9	10.089207	4
TATGCGA	85	6.606246E-4	10.056801	12
TCTATAC	85	6.619708E-4	10.054574	3
CCCTTAC	105	4.1055406E-5	9.952309	1
TCTTATA	205	4.1836756E-11	9.730558	2
CTTACAC	245	0.0	9.689783	3
ATGCGCC	80	0.0045235455	9.498089	11
ACCCCGT	130	4.2550237E-6	9.497039	10
AGGACCG	180	4.2109605E-9	9.495987	5
GTTAATC	80	0.004531816	9.495987	3
>>END_MODULE