FastQCFastQC Report
Mon 15 Aug 2016
SRR2921187_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921187_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1806999
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39580.21903719924582138No Hit
TATCAACGCAGAGTACTTTTTTTTT24130.1335363218241958No Hit
GGTATCAACGCAGAGTACTTTTTTT23080.1277255825819494No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCCG400.005282861614.248655
GGTATCA10600.012.8361511
CCCTACG853.875757E-612.3134121
ACTAGAC1103.7804057E-812.098813
CTAGACA1750.011.950334
ACACCGT1201.0124495E-811.8663176
GATCTAT657.919641E-411.7106571
CGTGTCA658.029037E-411.691524510
TAGACTG1900.011.498915
GTATCAA25050.011.3951011
CGTGGTT600.005879217311.0825912
TCGCACG600.005902281511.07676118
GTATAGG700.00147221710.8741821
TAGAACA1502.1100277E-910.7734034
CCCCTAG1158.6413274E-710.7559831
GTATAGA1355.558104E-810.5721211
CTTATAC2600.010.2468251
GTACTAG750.002613700210.1492361
GACCGCT750.002664042210.1259247
TAATACT2002.5465852E-119.9812414
Produced by FastQC (version 0.11.5)