##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921187_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1806999
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.137389672047412	31.0	30.0	33.0	25.0	34.0
2	29.44575010832878	31.0	30.0	34.0	25.0	34.0
3	29.587704254401913	31.0	30.0	34.0	25.0	34.0
4	33.23406487773374	35.0	35.0	37.0	28.0	37.0
5	32.73409393142995	35.0	33.0	37.0	26.0	37.0
6	32.660945578829875	35.0	33.0	37.0	25.0	37.0
7	32.50608550419784	35.0	33.0	37.0	24.0	37.0
8	32.43089287819196	36.0	33.0	37.0	23.0	37.0
9	33.78304027838421	38.0	34.0	39.0	23.0	39.0
10	33.64704795077363	38.0	34.0	39.0	22.0	39.0
11	33.65944253427921	38.0	34.0	39.0	22.0	39.0
12	33.52620117664703	38.0	34.0	39.0	19.0	39.0
13	33.49116463263123	38.0	34.0	39.0	18.0	39.0
14	34.4193073709504	39.0	34.0	41.0	17.0	41.0
15	34.38901847759739	39.0	34.0	41.0	17.0	41.0
16	34.3689620193481	39.0	34.0	41.0	17.0	41.0
17	34.336191110233045	39.0	34.0	41.0	17.0	41.0
18	34.28468084376361	39.0	34.0	41.0	17.0	41.0
19	34.315044446621165	39.0	34.0	41.0	18.0	41.0
20	34.240873957318186	39.0	34.0	41.0	17.0	41.0
21	34.16803053017738	39.0	34.0	41.0	16.0	41.0
22	34.07325294590645	39.0	34.0	41.0	10.0	41.0
23	33.944996095736634	39.0	34.0	41.0	8.0	41.0
24	33.85094402376537	39.0	34.0	41.0	2.0	41.0
25	33.71668440325645	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	73378.0
3	1.0
4	1162.0
5	10163.0
6	11234.0
7	8350.0
8	6506.0
9	5492.0
10	4887.0
11	4440.0
12	4099.0
13	3893.0
14	3721.0
15	3703.0
16	3643.0
17	4057.0
18	4243.0
19	5117.0
20	6446.0
21	8113.0
22	10051.0
23	12017.0
24	14219.0
25	16208.0
26	18452.0
27	20536.0
28	23259.0
29	28332.0
30	35511.0
31	44734.0
32	56988.0
33	73479.0
34	95998.0
35	126770.0
36	172568.0
37	261431.0
38	623798.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.24701876294519	18.702121141785955	9.50478255402289	31.546077541245964
2	14.868740934555028	20.559446906168734	34.839034221933716	29.73277793734252
3	18.461769283108662	23.142664637424243	27.258634845428976	31.136931234038123
4	13.541525354749437	16.167172298010016	32.42875249239615	37.862549854844396
5	15.050601379423398	37.09202395549161	32.06583440495059	15.791540260134399
6	34.729560924961575	34.38691491852264	16.02660141762798	14.856922738887803
7	30.665724378496574	31.322522107707524	18.77994342950465	19.231810084291258
8	28.205878918259558	34.477750799882	18.006075837835237	19.310294444023203
9	27.018301152745728	16.17452227184434	18.283886463123757	38.523290112286176
10	17.043324558146175	28.264422196261297	30.10599400439073	24.586259241201798
11	36.23971189145122	22.70083500339547	21.13056150062624	19.92889160452707
12	24.441641754206156	24.269366694078677	27.770761828760914	23.518229722954253
13	28.94508332221854	20.788023250858792	24.862125816522322	25.40476761040035
14	23.26582889696363	20.25116478930797	24.92494072227458	31.558065591453822
15	24.869242533858007	27.67751824017527	22.067055650178318	25.386183575788408
16	25.128882026249595	26.92838780553273	23.516936606369526	24.425793561848153
17	23.909050510215657	26.924356149843305	25.029316855019506	24.137276484921536
18	24.095089240251315	26.348012627794432	25.818349265666303	23.73854886628795
19	25.031851081940577	25.697320081512203	25.80535363832889	23.465475198218332
20	25.09896417588146	25.35436452353585	25.588292425926983	23.958378874655708
21	25.393725738950135	24.953574320662927	25.57422545915066	24.078474481236274
22	25.24981239166864	24.651679398944967	25.287334057940537	24.811174151445858
23	24.87905901521579	24.80949131455282	25.576341004597445	24.73510866563394
24	24.531473377238303	25.22004603767695	25.630112337909246	24.6183682471755
25	24.637310769329705	25.142572666739476	25.535184555861267	24.684932008069556
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	617.0
1	617.0
2	629.0
3	641.0
4	641.0
5	641.0
6	843.5
7	1046.0
8	1046.0
9	1046.0
10	1409.0
11	1772.0
12	1772.0
13	1772.0
14	2584.5
15	3397.0
16	3397.0
17	3397.0
18	6360.5
19	9324.0
20	9324.0
21	9324.0
22	15287.5
23	21251.0
24	21251.0
25	21251.0
26	32664.5
27	44078.0
28	44078.0
29	44078.0
30	58788.5
31	73499.0
32	73499.0
33	73499.0
34	91970.5
35	110442.0
36	110442.0
37	110442.0
38	132604.5
39	154767.0
40	154767.0
41	154767.0
42	175701.0
43	196635.0
44	196635.0
45	196635.0
46	217664.5
47	238694.0
48	238694.0
49	238694.0
50	247879.5
51	257065.0
52	257065.0
53	257065.0
54	246375.0
55	235685.0
56	235685.0
57	235685.0
58	209918.5
59	184152.0
60	184152.0
61	184152.0
62	157511.0
63	130870.0
64	130870.0
65	130870.0
66	103716.5
67	76563.0
68	76563.0
69	76563.0
70	58051.5
71	39540.0
72	39540.0
73	39540.0
74	28004.5
75	16469.0
76	16469.0
77	16469.0
78	11568.5
79	6668.0
80	6668.0
81	6668.0
82	4620.5
83	2573.0
84	2573.0
85	2573.0
86	1739.5
87	906.0
88	906.0
89	906.0
90	600.5
91	295.0
92	295.0
93	295.0
94	169.0
95	43.0
96	43.0
97	43.0
98	25.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09916994973433854
2	0.0
3	0.041062557311874553
4	0.05567241597809406
5	0.07288327220989055
6	0.009850586524951037
7	0.0027670186867839995
8	0.009407863535065597
9	4.9806336362112E-4
10	5.534037373567999E-4
11	0.009961267272422398
12	2.7670186867839996E-4
13	0.005423356626096639
14	0.06048702849309822
15	0.022689553231628794
16	0.006308802605867519
17	9.9612672724224E-4
18	0.010570011383514879
19	0.008467077181559037
20	0.0017708919595417595
21	0.019258450060016637
22	0.0023796360706342394
23	0.006972887090695679
24	0.012064201474378236
25	0.1760930692269337
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1806999.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.14086672769258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.15827038933142	35.87954755826219
2	14.903081234638673	15.243129825052653
3	6.120587368010039	9.390364286478006
4	2.981790687937099	6.099654407266638
5	1.6821952227814665	4.3014460849114045
6	1.0485932284691146	3.2175579929219933
7	0.6791603552373976	2.4312994449749668
8	0.4655576963587111	1.904721928282593
9	0.3390717747337815	1.5606381998504266
>10	1.5130562026327368	13.204274442578349
>50	0.07077371125018468	2.4687229377815014
>100	0.036012969164633424	3.0841165463147817
>500	9.789667701569631E-4	0.3593013174758655
>1k	8.701926845839673E-4	0.8552250278485767
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3958	0.21903719924582138	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2413	0.1335363218241958	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2308	0.1277255825819494	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.5340373735679986E-5
2	0.0	0.0	0.0	0.0	5.5340373735679986E-5
3	0.0	0.0	0.0	0.0	5.5340373735679986E-5
4	0.0	0.0	0.0	0.0	5.5340373735679986E-5
5	0.0	0.0	0.0	0.0	5.5340373735679986E-5
6	0.0	0.0	0.0	0.0	1.1068074747135997E-4
7	0.0	0.0	0.0	0.0	1.1068074747135997E-4
8	0.0	0.0	0.0	0.0	1.1068074747135997E-4
9	0.0	0.0	0.0	0.0	1.1068074747135997E-4
10	0.0	0.0	0.0	0.0	1.1068074747135997E-4
11	0.0	0.0	0.0	0.0	1.1068074747135997E-4
12	0.0	0.0	0.0	0.0	6.087441110924799E-4
13	0.0	0.0	0.0	0.0	7.747652322995199E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCCG	40	0.0052828616	14.24865	5
GGTATCA	1060	0.0	12.836151	1
CCCTACG	85	3.875757E-6	12.313412	1
ACTAGAC	110	3.7804057E-8	12.09881	3
CTAGACA	175	0.0	11.95033	4
ACACCGT	120	1.0124495E-8	11.866317	6
GATCTAT	65	7.919641E-4	11.710657	1
CGTGTCA	65	8.029037E-4	11.6915245	10
TAGACTG	190	0.0	11.49891	5
GTATCAA	2505	0.0	11.395101	1
CGTGGTT	60	0.0058792173	11.08259	12
TCGCACG	60	0.0059022815	11.076761	18
GTATAGG	70	0.001472217	10.874182	1
TAGAACA	150	2.1100277E-9	10.773403	4
CCCCTAG	115	8.6413274E-7	10.755983	1
GTATAGA	135	5.558104E-8	10.572121	1
CTTATAC	260	0.0	10.246825	1
GTACTAG	75	0.0026137002	10.149236	1
GACCGCT	75	0.0026640422	10.125924	7
TAATACT	200	2.5465852E-11	9.981241	4
>>END_MODULE