FastQCFastQC Report
Mon 15 Aug 2016
SRR2921186_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921186_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2341216
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28200.12045022757404698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14350.013.7076871
AGAACCG1102.764864E-912.9493315
TACACCG907.4984873E-611.6064385
TCTATAC1251.8424544E-811.3961423
GAACCGT1001.9416311E-611.3946826
GTATCAA35350.011.1558951
CGTTTAA700.00148839410.8601971
GTCCTAG2950.010.6301081
CTAGAAC1409.7421434E-810.1751273
CCAATAC1356.475693E-79.8485173
TAGAACC1451.6274316E-79.8236314
TAATACT2550.09.6823764
GTGTTAC3000.09.5026721
GATCTAT1201.6953507E-59.5026721
CATGGGT9600.09.4961774
CTATACT2002.6739144E-109.4961774
AGTACTA1304.2619395E-69.4961775
TAGACAG3000.09.1796375
GTATAAT2900.09.1749941
ACACCGT1451.7176917E-69.1681356
Produced by FastQC (version 0.11.5)