FastQCFastQC Report
Mon 15 Aug 2016
SRR2921186_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921186_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2341216
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47560.20314229870289627No Hit
GGTATCAACGCAGAGTACTTTTTTT30420.1299324795320039No Hit
TATCAACGCAGAGTACTTTTTTTTT29730.1269852931126389No Hit
GGTATCAACGCAGAGTACATGGGGG27940.11933969356095293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT604.040753E-412.6861171
GGTATCA17700.012.256081
TAAGGCG550.003071830112.0895495
CCCTACG1001.895447E-611.4175071
ATAAGAC1251.8195351E-811.40578653
CGAACCA600.005896135211.07853515
TATAACG803.754563E-410.69292454
AACCGTC803.7993136E-410.6792227
GTATCAA36950.010.377211
CCCTTAT1303.7633617E-710.246481
CCCTTAG1409.478936E-810.1942021
ACGATTC951.648273E-49.99780758
CCTAATA1606.586379E-89.5048222
ACGACAG1106.847077E-59.4989315
TAGCACC1752.6488124E-89.2332564
TAAGCTG1151.1122879E-49.0859355
AAAACCG950.00182604348.9989875
AGACTTA1901.07229425E-88.993036
CTAGCAC2651.8189894E-128.9668133
CCTACAC2553.6379788E-128.9457153
Produced by FastQC (version 0.11.5)