##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921186_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2341216
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.477480505856786	31.0	30.0	33.0	26.0	34.0
2	29.75953308024548	31.0	30.0	34.0	26.0	34.0
3	29.915285902710387	31.0	30.0	34.0	26.0	34.0
4	33.583569392999195	35.0	35.0	37.0	29.0	37.0
5	33.07769082391373	35.0	35.0	37.0	27.0	37.0
6	33.03603383882564	36.0	35.0	37.0	26.0	37.0
7	32.889388249525034	36.0	35.0	37.0	26.0	37.0
8	32.83783683350874	36.0	35.0	37.0	26.0	37.0
9	34.20684379399423	39.0	35.0	39.0	25.0	39.0
10	34.07680410521712	38.0	34.0	39.0	25.0	39.0
11	34.10650362888345	38.0	35.0	39.0	25.0	39.0
12	33.96352280182606	38.0	34.0	39.0	25.0	39.0
13	33.928227895247595	38.0	34.0	39.0	25.0	39.0
14	34.886557242048575	39.0	34.0	41.0	24.0	41.0
15	34.868245817558055	39.0	34.0	41.0	24.0	41.0
16	34.84775219373181	39.0	34.0	41.0	24.0	41.0
17	34.825697415360224	39.0	34.0	41.0	23.0	41.0
18	34.77855268373358	39.0	34.0	41.0	23.0	41.0
19	34.81269178068149	39.0	34.0	41.0	22.0	41.0
20	34.749498124051776	39.0	34.0	41.0	22.0	41.0
21	34.68359946284324	39.0	34.0	41.0	21.0	41.0
22	34.58950519729918	39.0	34.0	41.0	19.0	41.0
23	34.46169725475992	39.0	34.0	41.0	18.0	41.0
24	34.38260288670503	39.0	34.0	41.0	18.0	41.0
25	34.24172524021705	39.0	34.0	41.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	81013.0
3	3.0
4	1377.0
5	11214.0
6	12372.0
7	9174.0
8	7428.0
9	6396.0
10	5682.0
11	5000.0
12	4725.0
13	4416.0
14	4452.0
15	4415.0
16	4319.0
17	4563.0
18	5068.0
19	5932.0
20	7379.0
21	9704.0
22	12207.0
23	14605.0
24	17286.0
25	20026.0
26	22622.0
27	25581.0
28	29303.0
29	35475.0
30	45069.0
31	57600.0
32	72827.0
33	94173.0
34	124054.0
35	165573.0
36	226201.0
37	344188.0
38	839794.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.2717545828657	18.788092565870343	9.395756506867619	31.544396344396347
2	14.697490534833179	20.80585473531703	35.31882577258997	29.177828957259816
3	17.88991746908028	24.114752802483405	27.82759733115131	30.167732397285
4	13.442603473925471	16.269318758282978	33.10062998714896	37.187447780642586
5	14.80315795232299	37.47636493565108	32.23805681305305	15.48242029897288
6	33.92350984382128	34.84856250993096	16.54002026342452	14.687907382823246
7	30.052301903071694	31.785077406389416	19.50389007417089	18.658730616367997
8	27.84993740344091	34.678365595513036	18.55305629675254	18.918640704293512
9	27.545941937864765	15.739214151962058	18.649197681888385	38.06564622828479
10	16.98608757158673	28.676380137501194	30.688240640761038	23.64929165015103
11	36.00848286555128	22.579671022001634	21.54793075316399	19.863915359283098
12	24.255344231373783	24.786094063939423	28.267831759222556	22.690729945464238
13	28.89113463832637	21.11405497919241	24.957703549399394	25.037106833081822
14	23.230338855280095	20.412513525664146	25.513035065017203	30.844112554038556
15	24.97233721680359	27.809662962630675	22.48947959789635	24.728520222669385
16	24.883553952587825	26.954446263539456	23.829698536613286	24.332301247259434
17	23.605980823640365	27.059442614436257	25.4074805571122	23.927096004811172
18	23.69567232348377	26.669804676004095	26.167367237217704	23.467155763294432
19	24.906062367668284	25.984791436458778	26.0821781827206	23.026968013152334
20	25.30360291404125	25.38898589450952	25.697842488689638	23.609568702759592
21	25.299406121438615	25.162672662833913	25.663516087432527	23.87440512829494
22	25.286902190998184	24.84512321802004	25.516270177548762	24.35170441343302
23	24.879795011893428	25.017629780169138	25.61411646717572	24.48845874076172
24	24.451131480706312	25.428032018042185	25.743428527494682	24.377407973756824
25	24.416966936003483	25.20914746896694	25.828140121317084	24.545745473712493
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	722.0
1	722.0
2	721.5
3	721.0
4	721.0
5	721.0
6	1078.0
7	1435.0
8	1435.0
9	1435.0
10	1838.5
11	2242.0
12	2242.0
13	2242.0
14	3261.5
15	4281.0
16	4281.0
17	4281.0
18	8356.0
19	12431.0
20	12431.0
21	12431.0
22	21478.0
23	30525.0
24	30525.0
25	30525.0
26	46878.5
27	63232.0
28	63232.0
29	63232.0
30	83441.5
31	103651.0
32	103651.0
33	103651.0
34	127057.5
35	150464.0
36	150464.0
37	150464.0
38	179521.0
39	208578.0
40	208578.0
41	208578.0
42	234827.5
43	261077.0
44	261077.0
45	261077.0
46	287503.5
47	313930.0
48	313930.0
49	313930.0
50	324079.5
51	334229.0
52	334229.0
53	334229.0
54	315851.5
55	297474.0
56	297474.0
57	297474.0
58	264966.5
59	232459.0
60	232459.0
61	232459.0
62	197109.0
63	161759.0
64	161759.0
65	161759.0
66	126433.0
67	91107.0
68	91107.0
69	91107.0
70	66976.5
71	42846.0
72	42846.0
73	42846.0
74	29983.5
75	17121.0
76	17121.0
77	17121.0
78	12097.5
79	7074.0
80	7074.0
81	7074.0
82	4836.5
83	2599.0
84	2599.0
85	2599.0
86	1778.0
87	957.0
88	957.0
89	957.0
90	607.5
91	258.0
92	258.0
93	258.0
94	149.5
95	41.0
96	41.0
97	41.0
98	22.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09999077402512199
2	0.0
3	0.03763001790522532
4	0.05667994751445402
5	0.06287331027978622
6	0.0022210680261881004
7	4.271284665746347E-5
8	0.0014095239396962947
9	0.0
10	0.0
11	0.0011105340130940502
12	0.0
13	1.2813853997239042E-4
14	0.052109672922105436
15	0.022466957341825786
16	1.2813853997239042E-4
17	0.0
18	0.0012386725530664406
19	0.0014949496330112215
20	0.0
21	0.007047619698481472
22	0.0
23	2.989899266022443E-4
24	0.001537662479668685
25	0.16529871656438364
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2341216.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.30507280743163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.49905981448522	32.40353011764837
2	15.140352566834961	14.324309610086242
3	6.346714719477356	9.00695405678623
4	3.1753694657391147	6.008443350691363
5	1.8040875345444827	4.267124608630328
6	1.1840296672970274	3.3606365770586955
7	0.7681216130102025	2.543523417988662
8	0.5507974128179163	2.0844409372397203
9	0.38855968764863497	1.654275988282641
>10	2.0080073293356784	16.634578323351416
>50	0.09508302693771942	3.028186984870441
>100	0.036905136513808945	3.04313574997079
>500	0.0017290150562647882	0.5190296288941442
>1k	0.0011830103016543256	1.121830648500992
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4756	0.20314229870289627	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3042	0.1299324795320039	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2973	0.1269852931126389	No Hit
GGTATCAACGCAGAGTACATGGGGG	2794	0.11933969356095293	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.542569331492694E-5
2	0.0	0.0	0.0	0.0	8.542569331492694E-5
3	0.0	0.0	0.0	0.0	8.542569331492694E-5
4	0.0	0.0	0.0	0.0	8.542569331492694E-5
5	0.0	0.0	0.0	0.0	8.542569331492694E-5
6	0.0	0.0	0.0	0.0	8.542569331492694E-5
7	0.0	0.0	0.0	0.0	8.542569331492694E-5
8	0.0	0.0	0.0	0.0	8.542569331492694E-5
9	0.0	0.0	0.0	0.0	1.2813853997239042E-4
10	0.0	0.0	0.0	0.0	1.2813853997239042E-4
11	0.0	0.0	0.0	0.0	1.2813853997239042E-4
12	0.0	0.0	0.0	0.0	2.989899266022443E-4
13	0.0	0.0	0.0	0.0	2.989899266022443E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	60	4.040753E-4	12.686117	1
GGTATCA	1770	0.0	12.25608	1
TAAGGCG	55	0.0030718301	12.089549	5
CCCTACG	100	1.895447E-6	11.417507	1
ATAAGAC	125	1.8195351E-8	11.4057865	3
CGAACCA	60	0.0058961352	11.078535	15
TATAACG	80	3.754563E-4	10.6929245	4
AACCGTC	80	3.7993136E-4	10.679222	7
GTATCAA	3695	0.0	10.37721	1
CCCTTAT	130	3.7633617E-7	10.24648	1
CCCTTAG	140	9.478936E-8	10.194202	1
ACGATTC	95	1.648273E-4	9.9978075	8
CCTAATA	160	6.586379E-8	9.504822	2
ACGACAG	110	6.847077E-5	9.498931	5
TAGCACC	175	2.6488124E-8	9.233256	4
TAAGCTG	115	1.1122879E-4	9.085935	5
AAAACCG	95	0.0018260434	8.998987	5
AGACTTA	190	1.07229425E-8	8.99303	6
CTAGCAC	265	1.8189894E-12	8.966813	3
CCTACAC	255	3.6379788E-12	8.945715	3
>>END_MODULE