FastQCFastQC Report
Mon 15 Aug 2016
SRR2921185_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921185_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2635921
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27620.10478310996422124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16550.012.2859031
GTATAAT2650.010.7563931
GTATCAA34500.010.3276971
AAATTCG856.602855E-410.05771714
CGGAGCG951.6501038E-49.9968159
TAGACCG951.651368E-49.9960565
GGTTAGG1054.100126E-59.9539331
CAGGACT4100.09.7278694
GTCTAGG2303.6379788E-129.5014811
TAAGGCG900.00111808099.4962545
TCTTATA2451.8189894E-129.3065132
GTCTAGT1651.0637632E-79.2135571
CTTAGAC2401.0913936E-119.1009223
CGGACTG1151.1158518E-49.0833725
TAATACT4300.09.0545664
GTATTAC1054.4870915E-49.0490291
CCGTGCG1054.5084217E-49.0447379
ATAGCCG850.00746173138.937655
TGGACTG4800.08.9027385
GACCGTG2800.08.8181167
Produced by FastQC (version 0.11.5)