##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921185_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2635921
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.284199336778304	31.0	28.0	31.0	10.0	34.0
2	26.86882952865431	31.0	27.0	31.0	10.0	34.0
3	26.74391493523516	31.0	26.0	31.0	10.0	34.0
4	30.59255455683232	35.0	31.0	37.0	10.0	37.0
5	30.26625835903276	35.0	30.0	37.0	10.0	37.0
6	30.556874048956704	35.0	31.0	37.0	10.0	37.0
7	30.582104319514887	35.0	31.0	37.0	10.0	37.0
8	30.269874931760093	35.0	30.0	37.0	8.0	37.0
9	31.86825895009752	37.0	32.0	39.0	8.0	39.0
10	31.2553084861041	37.0	30.0	39.0	6.0	39.0
11	31.69085644068999	37.0	31.0	39.0	2.0	39.0
12	31.39326178591847	37.0	31.0	39.0	2.0	39.0
13	31.383237965022474	37.0	31.0	39.0	2.0	39.0
14	32.48639849221581	38.0	32.0	40.0	2.0	41.0
15	32.134186874341076	38.0	31.0	40.0	2.0	41.0
16	32.06084324985461	38.0	31.0	40.0	2.0	41.0
17	32.37973520450727	38.0	32.0	40.0	2.0	41.0
18	32.431202983700956	38.0	32.0	40.0	2.0	41.0
19	32.135062849000406	38.0	31.0	40.0	2.0	41.0
20	32.010136494986	38.0	31.0	40.0	2.0	41.0
21	31.953653770351995	38.0	31.0	40.0	2.0	41.0
22	31.187567078072522	38.0	30.0	40.0	2.0	41.0
23	31.288838322544567	38.0	30.0	40.0	2.0	41.0
24	30.913310376145567	38.0	30.0	40.0	2.0	41.0
25	30.414462345419306	37.0	29.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	212868.0
3	7.0
4	1614.0
5	9892.0
6	11555.0
7	10064.0
8	8746.0
9	8129.0
10	7505.0
11	7288.0
12	7017.0
13	6903.0
14	6741.0
15	7133.0
16	7678.0
17	8486.0
18	9680.0
19	11693.0
20	15037.0
21	19087.0
22	22613.0
23	26928.0
24	31021.0
25	35485.0
26	39774.0
27	44309.0
28	49689.0
29	58570.0
30	68543.0
31	82261.0
32	102658.0
33	130230.0
34	166453.0
35	214803.0
36	281581.0
37	371672.0
38	532208.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.27239800046451	17.97862780131552	12.448785811981121	25.300188386238847
2	15.884867477123349	19.926219290933908	39.12081847318949	25.06809475875325
3	18.64512312699035	23.6402834950528	30.181280356785784	27.533313021171065
4	13.803334505718318	15.959232873219653	35.09164522036854	35.14578740069349
5	14.123717598494506	36.57902325016694	34.03467036969587	15.26258878164269
6	33.66741214118111	34.116024667049835	17.303544702862965	14.913018488906088
7	29.22464944761442	31.42197553721015	20.88734521063494	18.466029804540486
8	26.10707028706965	34.54582082009928	20.551004675485494	18.79610421734558
9	26.930390528431623	15.780446034201875	19.363546481055575	37.925616956310925
10	15.915639636016671	28.134917398092064	32.976457175809564	22.9729857900817
11	35.18378908939972	22.90389075665005	22.255437638509108	19.656882515441122
12	23.318219086141344	24.752143925169108	29.491714354766074	22.437922633923474
13	28.50558594571051	21.563617103776497	25.19939508036128	24.73140187015171
14	22.69975917840642	21.061018887439904	25.901809899296584	30.3374120348571
15	24.457862408328495	28.082913152513306	23.12189606832159	24.337328370836605
16	24.198599786199672	27.080236599626055	25.021013832509414	23.700149781664855
17	23.192743764172334	27.329860814618733	25.872237877019703	23.605157544189222
18	23.11463229903603	26.993588702516707	26.653595654160284	23.238183344286977
19	24.494514100635783	26.286936289501284	26.668118645379746	22.550430964483184
20	24.806755109815306	25.851977373690772	26.237898005551852	23.10336951094207
21	25.420693805640166	25.445434361824564	25.77282932924633	23.361042503288935
22	24.23998576508675	24.95252815777383	27.00782654651975	23.79965953061967
23	24.481830192530825	25.484817831430995	26.003803415333408	24.029548560704768
24	24.171585150605015	25.685754785562192	26.204225469677905	23.938434594154884
25	23.830264040490274	25.498620342703827	26.66069577413257	24.010419842673333
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1302.0
1	1302.0
2	1334.5
3	1367.0
4	1367.0
5	1367.0
6	1632.0
7	1897.0
8	1897.0
9	1897.0
10	2618.0
11	3339.0
12	3339.0
13	3339.0
14	5449.5
15	7560.0
16	7560.0
17	7560.0
18	13683.0
19	19806.0
20	19806.0
21	19806.0
22	31326.0
23	42846.0
24	42846.0
25	42846.0
26	63325.0
27	83804.0
28	83804.0
29	83804.0
30	109803.0
31	135802.0
32	135802.0
33	135802.0
34	164680.5
35	193559.0
36	193559.0
37	193559.0
38	223972.0
39	254385.0
40	254385.0
41	254385.0
42	282039.5
43	309694.0
44	309694.0
45	309694.0
46	331441.5
47	353189.0
48	353189.0
49	353189.0
50	356697.0
51	360205.0
52	360205.0
53	360205.0
54	337435.5
55	314666.0
56	314666.0
57	314666.0
58	275680.5
59	236695.0
60	236695.0
61	236695.0
62	197776.0
63	158857.0
64	158857.0
65	158857.0
66	124345.5
67	89834.0
68	89834.0
69	89834.0
70	65416.5
71	40999.0
72	40999.0
73	40999.0
74	28659.0
75	16319.0
76	16319.0
77	16319.0
78	11423.0
79	6527.0
80	6527.0
81	6527.0
82	4430.0
83	2333.0
84	2333.0
85	2333.0
86	1522.5
87	712.0
88	712.0
89	712.0
90	447.0
91	182.0
92	182.0
93	182.0
94	106.5
95	31.0
96	31.0
97	31.0
98	21.0
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034485100274249496
2	0.05148105728510073
3	0.014795587576410673
4	0.01016722428327708
5	0.009142914374140955
6	6.069984646732584E-4
7	0.003072929727408371
8	0.0017071831818935394
9	0.006032047242690505
10	0.0032626167476187636
11	0.010015474667108764
12	0.008270354081173145
13	0.007549543404373652
14	0.013657465455148315
15	0.005728548010353877
16	0.028528927839643142
17	0.035888784223806404
18	0.021738132516111067
19	0.01657864556638837
20	0.015364648637041853
21	0.021700195112068988
22	0.00561473579822764
23	0.014985274596621067
24	0.010812160151992415
25	0.45832177823235215
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2635921.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.60466336834414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.52884675347204	32.40368497334752
2	12.671285786852474	11.810820170806865
3	6.025036354015159	8.42384373182736
4	3.4989777015871097	6.52274711663239
5	2.122954792389962	4.946979672277355
6	1.4390617719826875	4.024019366970358
7	0.9948430615936492	3.245502819293341
8	0.7211872078068486	2.6888549636315386
9	0.5510693441327008	2.311416114832684
>10	2.3330492180181723	17.728564072632484
>50	0.07895459616558696	2.5112596324214413
>100	0.03304294943010951	2.5817494576390785
>500	0.0011833237874325645	0.40313485348097117
>1k	5.07138766039281E-4	0.397423054206693
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2762	0.10478310996422124	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.793740404207865E-5	0.0
10	0.0	0.0	0.0	3.793740404207865E-5	0.0
11	0.0	0.0	0.0	3.793740404207865E-5	0.0
12	0.0	0.0	0.0	3.793740404207865E-5	0.0
13	0.0	0.0	0.0	3.793740404207865E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1655	0.0	12.285903	1
GTATAAT	265	0.0	10.756393	1
GTATCAA	3450	0.0	10.327697	1
AAATTCG	85	6.602855E-4	10.057717	14
CGGAGCG	95	1.6501038E-4	9.996815	9
TAGACCG	95	1.651368E-4	9.996056	5
GGTTAGG	105	4.100126E-5	9.953933	1
CAGGACT	410	0.0	9.727869	4
GTCTAGG	230	3.6379788E-12	9.501481	1
TAAGGCG	90	0.0011180809	9.496254	5
TCTTATA	245	1.8189894E-12	9.306513	2
GTCTAGT	165	1.0637632E-7	9.213557	1
CTTAGAC	240	1.0913936E-11	9.100922	3
CGGACTG	115	1.1158518E-4	9.083372	5
TAATACT	430	0.0	9.054566	4
GTATTAC	105	4.4870915E-4	9.049029	1
CCGTGCG	105	4.5084217E-4	9.044737	9
ATAGCCG	85	0.0074617313	8.93765	5
TGGACTG	480	0.0	8.902738	5
GACCGTG	280	0.0	8.818116	7
>>END_MODULE