FastQCFastQC Report
Mon 15 Aug 2016
SRR2921185_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921185_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2635921
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49790.18889033472550962No Hit
TATCAACGCAGAGTACTTTTTTTTT34110.12940448518753028No Hit
GGTATCAACGCAGAGTACTTTTTTT33220.1260280562277853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18050.013.3853921
CTATACG550.00306035812.0956824
AAGACCG1951.8189894E-1210.7162785
GTATCAA37500.010.019371
TTAATCC3150.09.956314
TACTAGC1356.4123924E-79.85574152
TAGGGTG2850.09.665165
GTCTATA1804.094545E-99.5120591
ACTAGAC1502.6348062E-79.503753
TAGACTG2700.09.4985195
TACCGTA1502.6758062E-79.4936557
AAAGCGT1201.71477E-59.4936546
TTAGACT1856.657501E-99.2468924
GTGACAC6900.09.2335419
ACCGTCC1252.7597227E-59.1187528
GAACCGT1252.77706E-59.1139096
CCCGTAG850.00736062468.9525261
GTCGAGC1502.7003243E-68.8673059
TAGGCTG3350.08.7896755
ACCGTAT1304.377457E-58.768038
Produced by FastQC (version 0.11.5)