##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921185_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2635921
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.69692187284824	31.0	30.0	33.0	26.0	34.0
2	29.98053507673409	31.0	30.0	34.0	26.0	34.0
3	30.13153239418025	31.0	30.0	34.0	26.0	34.0
4	33.821037125164224	35.0	35.0	37.0	30.0	37.0
5	33.348025984086775	35.0	35.0	37.0	28.0	37.0
6	33.32154112357692	36.0	35.0	37.0	28.0	37.0
7	33.19251980617022	36.0	35.0	37.0	27.0	37.0
8	33.15536808576585	37.0	35.0	37.0	27.0	37.0
9	34.55820944557898	39.0	35.0	39.0	27.0	39.0
10	34.43566783678266	38.0	35.0	39.0	27.0	39.0
11	34.46159084433866	38.0	35.0	39.0	27.0	39.0
12	34.33032515010883	38.0	35.0	39.0	27.0	39.0
13	34.30225526485809	38.0	35.0	39.0	26.0	39.0
14	35.2855518052324	39.0	35.0	41.0	26.0	41.0
15	35.277819024166504	39.0	35.0	41.0	26.0	41.0
16	35.25779907667946	39.0	35.0	41.0	26.0	41.0
17	35.23414548463327	39.0	35.0	41.0	25.0	41.0
18	35.19288362587498	39.0	35.0	41.0	25.0	41.0
19	35.23205703054075	39.0	35.0	41.0	25.0	41.0
20	35.1658570192354	39.0	35.0	41.0	25.0	41.0
21	35.10537910658172	39.0	35.0	41.0	25.0	41.0
22	35.01593370969767	39.0	35.0	41.0	25.0	41.0
23	34.89712134771869	39.0	35.0	41.0	24.0	41.0
24	34.819600435673145	39.0	35.0	41.0	24.0	41.0
25	34.683307276659654	39.0	35.0	41.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	78039.0
3	4.0
4	1238.0
5	10527.0
6	12022.0
7	8915.0
8	6906.0
9	6094.0
10	5398.0
11	5109.0
12	4550.0
13	4444.0
14	4410.0
15	4385.0
16	4447.0
17	4730.0
18	5161.0
19	6173.0
20	7856.0
21	10138.0
22	12826.0
23	15470.0
24	18562.0
25	21468.0
26	24769.0
27	27990.0
28	31992.0
29	39716.0
30	50314.0
31	64643.0
32	83111.0
33	107754.0
34	142517.0
35	189658.0
36	259533.0
37	395511.0
38	959541.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.802404473849506	19.444087894160013	9.399876502796156	31.35363112919433
2	14.760836914308129	20.974490510148065	35.222299909595165	29.04237266594864
3	17.616547865101335	24.001091491753467	28.276126160658784	30.106234482486414
4	13.47879707159426	16.615707669585277	33.26424925535335	36.64124600346711
5	14.785035220089496	37.45602233920916	32.437747979343214	15.321194461358118
6	33.97279108226754	35.11816215555877	16.433817545688697	14.475229216484998
7	29.644796304558298	31.85649206190229	19.758480607732626	18.740231025806782
8	27.452031511716356	35.05187141125521	18.637400909385796	18.858696167642634
9	26.986595930978076	15.591088638636435	19.08812723925952	38.33418819112597
10	16.66720411363172	28.780664352736178	31.210291363277854	23.341840170354246
11	35.553736202784826	22.72952488335866	21.896247825186336	19.820491088670178
12	23.6024234422896	24.940058878873412	28.824660839479193	22.632856839357796
13	28.6829440030593	21.404844049115784	25.033214537458637	24.878997410366274
14	22.871106998170056	20.84199562498126	25.672470291360455	30.614427085488234
15	24.57515437117155	28.231416549257425	22.573537140339738	24.61989193923128
16	24.57411804696946	27.07794693467047	24.324182826020728	24.023752192339344
17	23.302158726449274	27.24148736715194	25.82630033089192	23.630053575506864
18	23.215293113842083	26.856543213904573	26.568363258497346	23.359800413756002
19	24.621413309376535	26.13052458100898	26.32492063299361	22.923141476620877
20	24.806394473844048	25.810832804418677	26.097755568209884	23.28501715352739
21	25.263535303714384	25.302841749819784	25.91998330614258	23.51363964032325
22	24.949988561768535	25.160732760043352	25.63172991964405	24.257548758544058
23	24.49765525027799	25.341962427097652	25.849039925429334	24.311342397195027
24	24.25008441363821	25.77453781161911	25.814221706255715	24.161156068486967
25	24.169320678185603	25.541591251699092	25.975973864842445	24.31311420527286
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	722.0
1	722.0
2	771.0
3	820.0
4	820.0
5	820.0
6	1175.0
7	1530.0
8	1530.0
9	1530.0
10	2147.0
11	2764.0
12	2764.0
13	2764.0
14	4104.5
15	5445.0
16	5445.0
17	5445.0
18	11154.5
19	16864.0
20	16864.0
21	16864.0
22	27825.0
23	38786.0
24	38786.0
25	38786.0
26	58023.0
27	77260.0
28	77260.0
29	77260.0
30	101889.5
31	126519.0
32	126519.0
33	126519.0
34	153336.0
35	180153.0
36	180153.0
37	180153.0
38	211245.5
39	242338.0
40	242338.0
41	242338.0
42	269917.0
43	297496.0
44	297496.0
45	297496.0
46	322779.5
47	348063.0
48	348063.0
49	348063.0
50	358209.5
51	368356.0
52	368356.0
53	368356.0
54	348585.5
55	328815.0
56	328815.0
57	328815.0
58	290414.5
59	252014.0
60	252014.0
61	252014.0
62	212912.0
63	173810.0
64	173810.0
65	173810.0
66	136747.5
67	99685.0
68	99685.0
69	99685.0
70	72452.0
71	45219.0
72	45219.0
73	45219.0
74	31646.0
75	18073.0
76	18073.0
77	18073.0
78	12732.5
79	7392.0
80	7392.0
81	7392.0
82	5039.0
83	2686.0
84	2686.0
85	2686.0
86	1755.5
87	825.0
88	825.0
89	825.0
90	535.0
91	245.0
92	245.0
93	245.0
94	140.5
95	36.0
96	36.0
97	36.0
98	20.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10102730696405546
2	0.0
3	0.03774771702186826
4	0.05500923586101405
5	0.06180003118454612
6	0.003566115979955393
7	9.484351010519662E-4
8	0.0028832427071979775
9	1.8968702021039324E-4
10	7.58748080841573E-5
11	0.002390056454650955
12	3.793740404207865E-5
13	0.0010243099091361235
14	0.05379523893166753
15	0.020941447031227415
16	0.0014416213535989888
17	5.690610606311798E-4
18	0.0025797434748613484
19	0.002465931262735112
20	7.58748080841573E-4
21	0.008384166293299381
22	9.104976970098877E-4
23	0.0012139969293465168
24	0.0034523037678291573
25	0.1657864556638837
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2635921.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.18656187176927
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.42590009856404	19.973806486333697
2	14.924655710338937	10.204453317127133
3	8.00911761308115	8.214125844536275
4	4.822197808141675	6.594174549037822
5	3.26486779029556	5.580730235804289
6	2.240514212632385	4.595728665284128
7	1.603699160448882	3.8377472400673094
8	1.1972065443445328	3.2742700481217155
9	0.9179505288446466	2.8243415294613747
>10	4.3462982238064525	24.982631837440465
>50	0.17051401620216108	3.925237527215491
>100	0.07259792370642541	4.270948079949079
>500	0.0029122402355129607	0.6492426924231984
>1k	0.0015681293575804933	1.072561947198023
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4979	0.18889033472550962	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3411	0.12940448518753028	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3322	0.1260280562277853	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.793740404207865E-5	0.0
10	0.0	0.0	0.0	3.793740404207865E-5	0.0
11	0.0	0.0	0.0	3.793740404207865E-5	0.0
12	0.0	0.0	0.0	3.793740404207865E-5	0.0
13	0.0	0.0	0.0	3.793740404207865E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1805	0.0	13.385392	1
CTATACG	55	0.003060358	12.095682	4
AAGACCG	195	1.8189894E-12	10.716278	5
GTATCAA	3750	0.0	10.01937	1
TTAATCC	315	0.0	9.95631	4
TACTAGC	135	6.4123924E-7	9.8557415	2
TAGGGTG	285	0.0	9.66516	5
GTCTATA	180	4.094545E-9	9.512059	1
ACTAGAC	150	2.6348062E-7	9.50375	3
TAGACTG	270	0.0	9.498519	5
TACCGTA	150	2.6758062E-7	9.493655	7
AAAGCGT	120	1.71477E-5	9.493654	6
TTAGACT	185	6.657501E-9	9.246892	4
GTGACAC	690	0.0	9.23354	19
ACCGTCC	125	2.7597227E-5	9.118752	8
GAACCGT	125	2.77706E-5	9.113909	6
CCCGTAG	85	0.0073606246	8.952526	1
GTCGAGC	150	2.7003243E-6	8.867305	9
TAGGCTG	335	0.0	8.789675	5
ACCGTAT	130	4.377457E-5	8.76803	8
>>END_MODULE