FastQCFastQC Report
Mon 15 Aug 2016
SRR2921184_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921184_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3024052
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16700.011.7192881
TAAGGCG1157.1233444E-811.5603875
ATTCGAT909.5309886E-510.55446216
TAGCCCG909.561401E-510.55114655
GTCTTAA4000.010.4506451
CCCTACG1252.5699683E-69.8806111
GTTTAGG3100.09.8070581
TAGACTG3250.09.6421245
GTATTAA3750.09.6272621
CGTCTAT900.00111352479.5005871
TACTATA2500.09.49933052
TCGGAAC900.0011179139.4965023
TGAACCG1401.0654712E-69.4960315
GTATCAA31300.09.439881
GTGTAGC5250.09.4101061
GTAGGAC1856.7466317E-99.23984053
GTATTAG2600.09.135181
GGACCGC1151.1166821E-49.0828616
TACACGG950.00183144628.996245
GTATAGA3400.08.941731
Produced by FastQC (version 0.11.5)