##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921184_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3024052
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.19817020342243	31.0	28.0	31.0	10.0	34.0
2	26.799359270277098	31.0	27.0	31.0	10.0	34.0
3	26.66365756938042	31.0	26.0	31.0	10.0	34.0
4	30.548957160789563	35.0	31.0	37.0	10.0	37.0
5	30.23144972374814	35.0	30.0	37.0	10.0	37.0
6	30.57231753951321	35.0	31.0	37.0	10.0	37.0
7	30.437911781940258	35.0	31.0	37.0	8.0	37.0
8	30.110290762195888	35.0	30.0	37.0	7.0	37.0
9	31.686419413422787	37.0	31.0	39.0	2.0	39.0
10	31.032296402310543	37.0	30.0	39.0	2.0	39.0
11	31.53127955471665	37.0	31.0	39.0	2.0	39.0
12	31.210312521080986	37.0	30.0	39.0	2.0	39.0
13	31.229213981770155	37.0	30.0	39.0	2.0	39.0
14	32.32416770611087	38.0	31.0	40.0	2.0	41.0
15	31.98390040911995	38.0	31.0	40.0	2.0	41.0
16	31.910595122041553	38.0	31.0	40.0	2.0	41.0
17	32.232444415638355	38.0	31.0	40.0	2.0	41.0
18	32.28057123356344	38.0	32.0	40.0	2.0	41.0
19	31.983650744100963	38.0	31.0	40.0	2.0	41.0
20	31.851621268417343	38.0	31.0	40.0	2.0	41.0
21	31.804005685087425	38.0	31.0	40.0	2.0	41.0
22	31.043289930199613	38.0	30.0	40.0	2.0	41.0
23	31.15029007437703	38.0	30.0	40.0	2.0	41.0
24	30.777400983845517	38.0	30.0	40.0	2.0	41.0
25	30.287852193017844	37.0	28.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	253842.0
3	11.0
4	2144.0
5	12425.0
6	14382.0
7	12011.0
8	10426.0
9	9407.0
10	8950.0
11	8634.0
12	8357.0
13	8117.0
14	7915.0
15	8259.0
16	8635.0
17	9760.0
18	11363.0
19	13678.0
20	17267.0
21	21777.0
22	26571.0
23	31191.0
24	35774.0
25	40288.0
26	45123.0
27	50698.0
28	56442.0
29	66220.0
30	78205.0
31	93023.0
32	116282.0
33	146402.0
34	186841.0
35	241080.0
36	316708.0
37	415634.0
38	630210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.68968674599956	17.577857006287047	12.13145566617996	25.601000581533434
2	15.965127578680164	19.503621542445217	38.815900707103225	25.71535017177139
3	18.953776914353295	22.85605428461721	30.034108133332936	28.156060667696558
4	13.589265239452905	15.462305543420134	34.901167326499206	36.04726189062775
5	13.997720065441463	36.746367367105506	34.313180462409356	14.94273210504367
6	34.58013492586551	33.930439205153654	16.98557322990406	14.503852639076781
7	29.86701834785021	31.152819605873244	20.294277255153936	18.685884791122607
8	26.317053047200652	34.592138571199456	20.38736708437982	18.703441297220078
9	26.860444043483668	15.666197507282112	19.172425333548514	38.30093311568571
10	15.812066998462276	28.091535905024884	33.144099604821506	22.952297491691333
11	36.00602832200129	22.40741310310091	22.102524467321288	19.484034107576512
12	23.437396653217803	24.54881275216615	29.385045307229312	22.62874528738673
13	28.674911643248723	21.29487823518654	25.21491847566374	24.815291645901
14	22.75311482176261	20.62410206614085	25.845612261906968	30.777170850189574
15	24.542844698982723	27.8555609166156	23.026175458345833	24.575418926055843
16	24.238673422032516	27.05600763699909	24.922225857103307	23.783093083865086
17	23.34860345840545	26.928250260677178	25.88703018562332	23.83611609529405
18	23.263601315611908	26.769341902980237	26.42916017283944	23.537896608568413
19	24.441328171639014	26.12927497598026	26.480186403091725	22.949210449288998
20	24.778499108836982	25.84925073844655	26.018520447800736	23.353729704915736
21	25.185504671271236	25.57583018177705	25.644925173002083	23.593739973949635
22	24.14241489958725	25.007234109678695	26.951597440233027	23.89875355050103
23	24.54262706494173	25.44718630811504	25.894868718191592	24.115317908751635
24	24.080422630886527	25.49126475084052	26.238753114534845	24.189559503738103
25	23.7302585065836	25.33602400608126	26.774807435718877	24.158910051616264
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	944.0
1	944.0
2	1100.5
3	1257.0
4	1257.0
5	1257.0
6	1654.5
7	2052.0
8	2052.0
9	2052.0
10	2849.5
11	3647.0
12	3647.0
13	3647.0
14	5727.0
15	7807.0
16	7807.0
17	7807.0
18	14207.5
19	20608.0
20	20608.0
21	20608.0
22	33014.5
23	45421.0
24	45421.0
25	45421.0
26	67425.5
27	89430.0
28	89430.0
29	89430.0
30	118024.5
31	146619.0
32	146619.0
33	146619.0
34	180945.0
35	215271.0
36	215271.0
37	215271.0
38	252994.0
39	290717.0
40	290717.0
41	290717.0
42	323112.5
43	355508.0
44	355508.0
45	355508.0
46	380553.0
47	405598.0
48	405598.0
49	405598.0
50	409430.5
51	413263.0
52	413263.0
53	413263.0
54	390595.5
55	367928.0
56	367928.0
57	367928.0
58	326795.5
59	285663.0
60	285663.0
61	285663.0
62	237784.0
63	189905.0
64	189905.0
65	189905.0
66	147109.0
67	104313.0
68	104313.0
69	104313.0
70	76313.5
71	48314.0
72	48314.0
73	48314.0
74	34000.0
75	19686.0
76	19686.0
77	19686.0
78	13304.5
79	6923.0
80	6923.0
81	6923.0
82	4599.0
83	2275.0
84	2275.0
85	2275.0
86	1482.0
87	689.0
88	689.0
89	689.0
90	434.0
91	179.0
92	179.0
93	179.0
94	101.5
95	24.0
96	24.0
97	24.0
98	17.5
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03339889657982072
2	0.05691039704343708
3	0.014318536850556804
4	0.010085805402817147
5	0.009292168256365961
6	6.944325031447872E-4
7	0.0033398896579820716
8	0.0015542060784669047
9	0.006745915744835075
10	0.0033729578724175373
11	0.010747169691526469
12	0.008333190037737448
13	0.0076387575345926596
14	0.014682287209346927
15	0.006249892528303084
16	0.028934687631032803
17	0.03822685588739876
18	0.022089567242891326
19	0.01643490257442663
20	0.015707401856846378
21	0.022420249387245985
22	0.006051483241690288
23	0.015872742929023707
24	0.010780237905961934
25	0.4678490978329738
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3024052.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.56449465565185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.13561285838432	32.65829370115104
2	12.106136104079376	11.27432219837996
3	5.877843853065929	8.210964860485335
4	3.392896625180263	6.319540670815423
5	2.211024405272359	5.147761705141027
6	1.4254111199015085	3.9824129084856303
7	1.013974503469281	3.30506472434338
8	0.7455759217804728	2.777389282010362
9	0.5536046366936918	2.3200488132000743
>10	2.4332058712285654	18.18498470451557
>50	0.07091233627761732	2.24072834443522
>100	0.03137840545012534	2.607392911219464
>500	0.0019093133221781183	0.598851713623078
>1k	5.140458943221964E-4	0.37224346219443577
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.3068214435466057E-5	0.0
3	0.0	0.0	0.0	3.3068214435466057E-5	0.0
4	0.0	0.0	0.0	3.3068214435466057E-5	0.0
5	0.0	0.0	0.0	3.3068214435466057E-5	0.0
6	0.0	0.0	0.0	3.3068214435466057E-5	0.0
7	0.0	3.3068214435466057E-5	0.0	3.3068214435466057E-5	0.0
8	0.0	3.3068214435466057E-5	0.0	3.3068214435466057E-5	0.0
9	0.0	3.3068214435466057E-5	0.0	3.3068214435466057E-5	0.0
10	0.0	3.3068214435466057E-5	0.0	3.3068214435466057E-5	0.0
11	3.3068214435466057E-5	3.3068214435466057E-5	0.0	3.3068214435466057E-5	0.0
12	3.3068214435466057E-5	3.3068214435466057E-5	0.0	3.3068214435466057E-5	0.0
13	6.613642887093211E-5	9.920464330639818E-5	0.0	3.3068214435466057E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1670	0.0	11.719288	1
TAAGGCG	115	7.1233444E-8	11.560387	5
ATTCGAT	90	9.5309886E-5	10.554462	16
TAGCCCG	90	9.561401E-5	10.5511465	5
GTCTTAA	400	0.0	10.450645	1
CCCTACG	125	2.5699683E-6	9.880611	1
GTTTAGG	310	0.0	9.807058	1
TAGACTG	325	0.0	9.642124	5
GTATTAA	375	0.0	9.627262	1
CGTCTAT	90	0.0011135247	9.500587	1
TACTATA	250	0.0	9.4993305	2
TCGGAAC	90	0.001117913	9.496502	3
TGAACCG	140	1.0654712E-6	9.496031	5
GTATCAA	3130	0.0	9.43988	1
GTGTAGC	525	0.0	9.410106	1
GTAGGAC	185	6.7466317E-9	9.2398405	3
GTATTAG	260	0.0	9.13518	1
GGACCGC	115	1.1166821E-4	9.082861	6
TACACGG	95	0.0018314462	8.99624	5
GTATAGA	340	0.0	8.94173	1
>>END_MODULE