FastQCFastQC Report
Mon 15 Aug 2016
SRR2921184_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921184_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3024052
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46530.15386640176822355No Hit
TATCAACGCAGAGTACTTTTTTTTT31360.10370192046962157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16450.012.3739981
TTGATCG600.005894580411.07909815
GTCCTAA1909.094947E-1210.5130281
CCCCTAT1201.5009464E-610.3044371
TCTATAC2250.010.1375093
GTATCAA33700.010.0762841
GGAACCG856.605925E-410.0573085
GTCCTAG3950.09.8729951
TAAGCTG2650.09.6777875
CGATCGC800.00448256969.50895219
ATTAGAC1106.8096684E-59.5039153
CGGTGTT1106.8343725E-59.5007714
CTATACT2800.09.164494
ACTGCGC1151.1132372E-49.0854378
GAACGGT1054.5237248E-49.0417696
ATAGCAC3900.09.0165343
CCTCTAG4550.08.98916151
CCCTAGA6900.08.960381
TTTGCGC850.0074767038.93554217
AATACTG3300.08.9228985
Produced by FastQC (version 0.11.5)