##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921184_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3024052
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.48594104863276	31.0	30.0	33.0	26.0	34.0
2	29.775725748102214	31.0	30.0	34.0	26.0	34.0
3	29.935121486006192	31.0	30.0	34.0	26.0	34.0
4	33.6096571752073	35.0	35.0	37.0	30.0	37.0
5	33.0973326516872	35.0	35.0	37.0	27.0	37.0
6	33.05717395071249	36.0	35.0	37.0	26.0	37.0
7	32.912205874766705	36.0	35.0	37.0	26.0	37.0
8	32.870303486844804	36.0	35.0	37.0	26.0	37.0
9	34.25753988357343	38.0	35.0	39.0	26.0	39.0
10	34.127762683975014	38.0	34.0	39.0	25.0	39.0
11	34.15546723402905	38.0	35.0	39.0	25.0	39.0
12	34.00933218079583	38.0	34.0	39.0	25.0	39.0
13	33.98073181281274	38.0	34.0	39.0	25.0	39.0
14	34.94635045958204	39.0	34.0	41.0	25.0	41.0
15	34.94376287180247	39.0	35.0	41.0	25.0	41.0
16	34.92224935285504	39.0	35.0	41.0	24.0	41.0
17	34.89232559493025	39.0	35.0	41.0	24.0	41.0
18	34.84373482995663	39.0	35.0	41.0	24.0	41.0
19	34.88132942158402	39.0	35.0	41.0	23.0	41.0
20	34.81085940321132	39.0	34.0	41.0	23.0	41.0
21	34.74591640619936	39.0	34.0	41.0	22.0	41.0
22	34.651002033033826	39.0	34.0	41.0	21.0	41.0
23	34.527095102861985	39.0	34.0	41.0	18.0	41.0
24	34.44930774999901	39.0	34.0	41.0	18.0	41.0
25	34.31290864045989	39.0	34.0	41.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	101608.0
3	7.0
4	1645.0
5	14110.0
6	15755.0
7	11678.0
8	8954.0
9	7875.0
10	7040.0
11	6324.0
12	5973.0
13	5725.0
14	5413.0
15	5454.0
16	5593.0
17	5900.0
18	6523.0
19	7651.0
20	9515.0
21	12280.0
22	15541.0
23	19153.0
24	22668.0
25	26317.0
26	29459.0
27	33239.0
28	37830.0
29	46269.0
30	58553.0
31	75170.0
32	95942.0
33	123280.0
34	161423.0
35	215107.0
36	293110.0
37	443855.0
38	1082113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.94842938002714	18.924166694250438	9.184270629903017	31.943133295819404
2	14.70679075624361	20.75893536222261	35.01510556035412	29.519168321179663
3	17.76017979799851	23.18356121243237	28.194729118727814	30.86152987084131
4	13.339884526940956	16.11623405628071	33.14804704948136	37.39583436729697
5	14.688508598879334	37.0757060068071	32.96990408776833	15.265881306545234
6	34.576982476098514	34.814095844067374	16.22424244428497	14.384679235549147
7	29.831255832533614	31.6001462969736	19.52962083819171	19.038977032301073
8	27.411348643525447	35.07190128669638	18.58176348967614	18.93498658010204
9	26.73677603812387	15.562673340306352	18.84222977430501	38.858320847264764
10	16.414757604146242	28.637045595611966	31.572323258844705	23.37587354139709
11	36.318669725893216	22.239966294713575	21.747449781041304	19.693914198351905
12	23.596863275227957	24.62313734645571	28.837854979719225	22.94214439859711
13	28.716074107192462	21.04490030238655	25.020337678210975	25.218687912210015
14	22.770609807583124	20.422472766639448	25.584933278101214	31.221984147676213
15	24.529513811368933	28.01084204450927	22.548376587401993	24.911267556719803
16	24.684446819599614	26.97314987023363	24.123514829875102	24.21888848029165
17	23.34123449996792	26.866038850195075	25.830631085843837	23.962095563993167
18	23.34038724766052	26.642002769314516	26.34737865894936	23.67023132407561
19	24.613397885519078	26.033049925427676	25.94452215839757	23.409030030655675
20	24.798570641065364	25.753791402351776	25.862322681813865	23.58531527476899
21	24.96791175559159	25.355550315458615	25.70032738111971	23.97621054783009
22	24.74699858961407	25.192204352189734	25.572363710918832	24.48843334727736
23	24.438439681787997	25.3020275829592	25.715070888681357	24.544461846571444
24	24.12124066211241	25.569334590455767	25.801026973345454	24.50839777408637
25	24.18221373671582	25.391420481721788	25.847233060969778	24.579132720592618
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	811.0
1	811.0
2	919.5
3	1028.0
4	1028.0
5	1028.0
6	1325.5
7	1623.0
8	1623.0
9	1623.0
10	2282.0
11	2941.0
12	2941.0
13	2941.0
14	4366.5
15	5792.0
16	5792.0
17	5792.0
18	11529.0
19	17266.0
20	17266.0
21	17266.0
22	28516.5
23	39767.0
24	39767.0
25	39767.0
26	60836.0
27	81905.0
28	81905.0
29	81905.0
30	108440.0
31	134975.0
32	134975.0
33	134975.0
34	167333.5
35	199692.0
36	199692.0
37	199692.0
38	237139.5
39	274587.0
40	274587.0
41	274587.0
42	307916.5
43	341246.0
44	341246.0
45	341246.0
46	371539.5
47	401833.0
48	401833.0
49	401833.0
50	412197.0
51	422561.0
52	422561.0
53	422561.0
54	402664.0
55	382767.0
56	382767.0
57	382767.0
58	343946.5
59	305126.0
60	305126.0
61	305126.0
62	256427.5
63	207729.0
64	207729.0
65	207729.0
66	162028.0
67	116327.0
68	116327.0
69	116327.0
70	84728.0
71	53129.0
72	53129.0
73	53129.0
74	37389.0
75	21649.0
76	21649.0
77	21649.0
78	14636.0
79	7623.0
80	7623.0
81	7623.0
82	5111.5
83	2600.0
84	2600.0
85	2600.0
86	1721.0
87	842.0
88	842.0
89	842.0
90	517.5
91	193.0
92	193.0
93	193.0
94	114.0
95	35.0
96	35.0
97	35.0
98	20.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0976173690134958
2	0.0
3	0.03650730873675453
4	0.05628210096916323
5	0.06329256242948204
6	0.006018415027254823
7	0.00218250215274076
8	0.006084551456125755
9	4.960232165319909E-4
10	3.637503587901266E-4
11	0.006415233600480415
12	1.9840928661279633E-4
13	0.0035713671590303342
14	0.05476096310513179
15	0.02106445259539188
16	0.005158641451932705
17	8.597735753221175E-4
18	0.006580574672657745
19	0.00469568644983618
20	0.0012896603629831763
21	0.011871488982332314
22	0.0018848882228215652
23	0.005158641451932705
24	0.007109666103625202
25	0.16692834647023264
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3024052.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.6152547435519
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.9742632547024	20.760244006183672
2	14.203224731967149	9.83296484553919
3	7.590382190547709	7.882290393801854
4	4.651084494367189	6.439938984252182
5	3.050040339908193	5.278896167201586
6	2.1311082393084506	4.4261312753846624
7	1.5865752826998556	3.8443795306334865
8	1.2048161019078627	3.3364013029339077
9	0.9038046592172398	2.815688566549248
>10	4.481912846830952	25.834166854549924
>50	0.1505066827492933	3.512367167482716
>100	0.06648976726812629	4.04922766347834
>500	0.00405398596800811	0.9230356826257166
>1k	0.0017374225574809253	1.0642675593834863
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4653	0.15386640176822355	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3136	0.10370192046962157	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.3068214435466057E-5	0.0
3	0.0	0.0	0.0	3.3068214435466057E-5	0.0
4	0.0	0.0	0.0	3.3068214435466057E-5	0.0
5	3.3068214435466057E-5	6.613642887093211E-5	0.0	3.3068214435466057E-5	0.0
6	3.3068214435466057E-5	6.613642887093211E-5	0.0	3.3068214435466057E-5	0.0
7	3.3068214435466057E-5	6.613642887093211E-5	0.0	3.3068214435466057E-5	0.0
8	3.3068214435466057E-5	6.613642887093211E-5	0.0	3.3068214435466057E-5	0.0
9	3.3068214435466057E-5	6.613642887093211E-5	0.0	3.3068214435466057E-5	0.0
10	3.3068214435466057E-5	6.613642887093211E-5	0.0	3.3068214435466057E-5	0.0
11	3.3068214435466057E-5	6.613642887093211E-5	0.0	3.3068214435466057E-5	0.0
12	3.3068214435466057E-5	6.613642887093211E-5	0.0	3.3068214435466057E-5	0.0
13	3.3068214435466057E-5	6.613642887093211E-5	0.0	3.3068214435466057E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1645	0.0	12.373998	1
TTGATCG	60	0.0058945804	11.079098	15
GTCCTAA	190	9.094947E-12	10.513028	1
CCCCTAT	120	1.5009464E-6	10.304437	1
TCTATAC	225	0.0	10.137509	3
GTATCAA	3370	0.0	10.076284	1
GGAACCG	85	6.605925E-4	10.057308	5
GTCCTAG	395	0.0	9.872995	1
TAAGCTG	265	0.0	9.677787	5
CGATCGC	80	0.0044825696	9.508952	19
ATTAGAC	110	6.8096684E-5	9.503915	3
CGGTGTT	110	6.8343725E-5	9.50077	14
CTATACT	280	0.0	9.16449	4
ACTGCGC	115	1.1132372E-4	9.085437	8
GAACGGT	105	4.5237248E-4	9.041769	6
ATAGCAC	390	0.0	9.016534	3
CCTCTAG	455	0.0	8.9891615	1
CCCTAGA	690	0.0	8.96038	1
TTTGCGC	85	0.007476703	8.935542	17
AATACTG	330	0.0	8.922898	5
>>END_MODULE