FastQCFastQC Report
Mon 15 Aug 2016
SRR2921183_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921183_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2412094
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31430.13030172124303613No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTACG500.001501237213.2985042
GGTATCA15850.012.4091481
TACGACT701.0954923E-412.2093684
CTTAGAC1800.011.6066763
TAGACTG2450.010.8525465
GTATCAA32850.010.6442071
ACGCATA1054.1259125E-59.9481687
TAGGACA2700.09.8478844
CGTGCGC1701.6698323E-89.49853710
TCAATAC1502.6642374E-79.4963713
TAGGTTG1304.2632746E-69.4959775
GTATTAG2054.2200554E-109.2699421
GTCTTAT1951.6771082E-99.2580581
GTCTTAG2900.09.17404651
TAATACC1252.7680799E-59.1163284
TGGACCG1054.512796E-49.0437895
GTCTAGA2651.8189894E-128.963861
TATGCCG850.0074631228.937395
CTAGGCC1606.780356E-78.9028483
GTTCTAA2252.6193447E-108.8682441
Produced by FastQC (version 0.11.5)