##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921183_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2412094
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.529887309532715	31.0	27.0	31.0	2.0	34.0
2	26.27037627886807	31.0	26.0	31.0	2.0	34.0
3	25.91114027894435	31.0	26.0	31.0	2.0	34.0
4	29.701376480352756	35.0	30.0	37.0	2.0	37.0
5	29.532111518042	35.0	30.0	37.0	2.0	37.0
6	29.751707437604008	35.0	30.0	37.0	2.0	37.0
7	29.72842642119254	35.0	30.0	37.0	2.0	37.0
8	29.35643594320951	35.0	29.0	37.0	2.0	37.0
9	30.87898149906264	37.0	30.0	39.0	2.0	39.0
10	30.26140440629594	37.0	29.0	39.0	2.0	39.0
11	30.717766803449617	37.0	30.0	39.0	2.0	39.0
12	30.402512505731533	37.0	30.0	39.0	2.0	39.0
13	30.40705130065412	37.0	30.0	39.0	2.0	39.0
14	31.457643027178875	38.0	30.0	40.0	2.0	41.0
15	31.114124491002425	38.0	29.0	40.0	2.0	41.0
16	31.03978990868515	38.0	29.0	40.0	2.0	41.0
17	31.338509610321985	38.0	30.0	40.0	2.0	41.0
18	31.383253306048605	38.0	30.0	40.0	2.0	41.0
19	31.08029662193928	38.0	30.0	40.0	2.0	41.0
20	30.95030707758487	38.0	29.0	40.0	2.0	41.0
21	30.89629923211948	38.0	30.0	40.0	2.0	41.0
22	30.151346091818976	37.0	27.0	40.0	2.0	41.0
23	30.236721703217203	37.0	27.0	40.0	2.0	41.0
24	29.87049551136896	37.0	27.0	40.0	2.0	41.0
25	29.394640092799037	37.0	26.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	249894.0
3	20.0
4	1836.0
5	10451.0
6	12743.0
7	11007.0
8	9140.0
9	8397.0
10	7817.0
11	7380.0
12	7184.0
13	7081.0
14	7118.0
15	7181.0
16	7471.0
17	8356.0
18	9485.0
19	11594.0
20	14689.0
21	18311.0
22	22057.0
23	25569.0
24	29201.0
25	32671.0
26	36332.0
27	40223.0
28	44555.0
29	52265.0
30	61220.0
31	72227.0
32	90229.0
33	113466.0
34	144575.0
35	186416.0
36	244395.0
37	322980.0
38	476558.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.578646524742574	17.653545468834302	12.151591949368159	25.616216057054963
2	15.864438754325832	19.506787561177646	39.11325128888377	25.515522395612756
3	18.817492764560026	23.742899317505202	29.56305406055379	27.876553857380976
4	13.964879867453675	15.715496410203677	34.464200716798324	35.855423005544324
5	13.978097392923882	36.554251305735534	34.44508986636449	15.022561434976092
6	33.82698749626878	34.68491923982621	16.744179297535737	14.743913966369274
7	29.49163897901598	31.58013222079696	19.890457233877246	19.037771566309818
8	27.08480078273181	33.74502760083165	20.31520709433618	18.85496452210036
9	27.131061085995363	15.77254695797249	18.906450564358046	38.189941391674104
10	16.247135698350633	27.8497391604295	32.377025222438135	23.526099918781735
11	35.51323284846128	22.65872639094069	21.84926108251836	19.978779678079672
12	23.531601873035275	24.6864108753463	28.91371748743944	22.86826976417899
13	28.574289387739704	21.279539483203898	25.193923957844007	24.952247171212395
14	22.805220781956308	21.00851416359766	25.530632164415323	30.65563289003071
15	24.88009691751232	27.818012660306106	22.869937494506516	24.431952927675056
16	24.513217856702983	27.12626747068193	24.651146202802774	23.70936846981231
17	23.396132669092044	27.248657499340567	25.56163558634556	23.793574245221826
18	23.5709614777765	26.717668041013965	26.263855606039055	23.44751487517048
19	24.609103050673447	26.327478120178448	26.389135948670166	22.674282880477936
20	24.875442006727127	25.674163918982572	26.1782008584716	23.2721932158187
21	25.385337219567678	25.51910794086389	25.551700559257817	23.543854280310615
22	24.28232045573273	24.89075184291483	26.881970529946848	23.94495717140559
23	24.718263460515423	25.189666045952734	26.01342848459141	24.078642008940438
24	24.34574359135956	25.509345217018502	25.9895216147792	24.155389576842737
25	23.8854004578184	25.115620803747845	26.828566335353642	24.17041240308011
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1332.0
1	1332.0
2	1497.5
3	1663.0
4	1663.0
5	1663.0
6	2161.5
7	2660.0
8	2660.0
9	2660.0
10	3369.5
11	4079.0
12	4079.0
13	4079.0
14	5748.5
15	7418.0
16	7418.0
17	7418.0
18	12763.0
19	18108.0
20	18108.0
21	18108.0
22	28055.5
23	38003.0
24	38003.0
25	38003.0
26	55195.0
27	72387.0
28	72387.0
29	72387.0
30	94499.0
31	116611.0
32	116611.0
33	116611.0
34	141931.0
35	167251.0
36	167251.0
37	167251.0
38	195190.5
39	223130.0
40	223130.0
41	223130.0
42	249731.5
43	276333.0
44	276333.0
45	276333.0
46	299111.5
47	321890.0
48	321890.0
49	321890.0
50	325647.0
51	329404.0
52	329404.0
53	329404.0
54	309858.0
55	290312.0
56	290312.0
57	290312.0
58	258350.5
59	226389.0
60	226389.0
61	226389.0
62	190366.0
63	154343.0
64	154343.0
65	154343.0
66	121470.0
67	88597.0
68	88597.0
69	88597.0
70	65755.5
71	42914.0
72	42914.0
73	42914.0
74	30544.0
75	18174.0
76	18174.0
77	18174.0
78	12663.0
79	7152.0
80	7152.0
81	7152.0
82	4900.5
83	2649.0
84	2649.0
85	2649.0
86	1811.0
87	973.0
88	973.0
89	973.0
90	631.5
91	290.0
92	290.0
93	290.0
94	159.0
95	28.0
96	28.0
97	28.0
98	16.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03337349207783776
2	0.05335612957040646
3	0.014676044963421824
4	0.010696100566561667
5	0.009742572221480589
6	5.804085578754393E-4
7	0.0028191272811092767
8	0.0012022748698848386
9	0.006218663120093994
10	0.003109331560046997
11	0.010571727304159789
12	0.007462395744112792
13	0.008084262056122192
14	0.014427298438618065
15	0.0060528321035581535
16	0.028896054631370086
17	0.03822404931151108
18	0.021765320920328974
19	0.0164587283911821
20	0.015173538013029344
21	0.020936165837649776
22	0.0063844941366298326
23	0.014717502717555783
24	0.011276509124437107
25	0.46192229656058176
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2412094.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.862931262030564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.26596968519938	34.58861139626683
2	11.276884638526061	10.794895086072428
3	5.327490479137388	7.649679318062253
4	3.121225727611833	5.97564049815866
5	1.9989727477949482	4.783834761119099
6	1.3677125178162204	3.9277638135873914
7	0.9423718235497224	3.1573274469684334
8	0.688173119661292	2.6350386178180445
9	0.5310961370792315	2.28778361122979
>10	2.3710680899862013	18.689418382356223
>50	0.08115467637346772	2.649858807506456
>100	0.026179910136965384	1.9847947732556503
>500	0.0010739666067402609	0.38445302428184935
>1k	6.264805205984554E-4	0.49090046331697323
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3143	0.13030172124303613	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.145775413395995E-5
2	0.0	0.0	0.0	0.0	4.145775413395995E-5
3	0.0	0.0	0.0	0.0	4.145775413395995E-5
4	0.0	0.0	0.0	0.0	4.145775413395995E-5
5	0.0	0.0	0.0	0.0	4.145775413395995E-5
6	0.0	0.0	0.0	0.0	4.145775413395995E-5
7	0.0	0.0	0.0	0.0	4.145775413395995E-5
8	0.0	0.0	0.0	0.0	4.145775413395995E-5
9	0.0	0.0	0.0	0.0	4.145775413395995E-5
10	0.0	0.0	0.0	4.145775413395995E-5	4.145775413395995E-5
11	0.0	0.0	0.0	4.145775413395995E-5	4.145775413395995E-5
12	0.0	0.0	0.0	4.145775413395995E-5	4.145775413395995E-5
13	0.0	0.0	0.0	4.145775413395995E-5	4.145775413395995E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTACG	50	0.0015012372	13.298504	2
GGTATCA	1585	0.0	12.409148	1
TACGACT	70	1.0954923E-4	12.209368	4
CTTAGAC	180	0.0	11.606676	3
TAGACTG	245	0.0	10.852546	5
GTATCAA	3285	0.0	10.644207	1
ACGCATA	105	4.1259125E-5	9.948168	7
TAGGACA	270	0.0	9.847884	4
CGTGCGC	170	1.6698323E-8	9.498537	10
TCAATAC	150	2.6642374E-7	9.496371	3
TAGGTTG	130	4.2632746E-6	9.495977	5
GTATTAG	205	4.2200554E-10	9.269942	1
GTCTTAT	195	1.6771082E-9	9.258058	1
GTCTTAG	290	0.0	9.1740465	1
TAATACC	125	2.7680799E-5	9.116328	4
TGGACCG	105	4.512796E-4	9.043789	5
GTCTAGA	265	1.8189894E-12	8.96386	1
TATGCCG	85	0.007463122	8.93739	5
CTAGGCC	160	6.780356E-7	8.902848	3
GTTCTAA	225	2.6193447E-10	8.868244	1
>>END_MODULE