FastQCFastQC Report
Mon 15 Aug 2016
SRR2921183_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921183_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2412094
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54510.22598621778421568No Hit
GGTATCAACGCAGAGTACTTTTTTT37720.15637864859329695No Hit
TATCAACGCAGAGTACTTTTTTTTT36430.1510305983100161No Hit
CTTATACACATCTCCGAGCCCACGA28410.11778147949458022No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26850.11131406984968246No Hit
GGTATCAACGCAGAGTACATGGGGG24190.10028630725004911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19450.013.2050051
GTACTAG853.8883027E-612.31028651
TTATACA5400.011.9670132
CTTATAC5350.011.5572361
TATACAC5700.011.1704463
GAGCGCG600.005871522711.08478511
AAGACCG1855.456968E-1210.7816185
ACACCGT1002.417894E-510.4429216
GTATAGA1502.3970642E-810.146661
TACGCTG856.607942E-410.0568045
GTATATA2500.09.8929941
ACTATAC1252.5600948E-69.8833453
CAATACC1356.418177E-79.8549834
TTAGGGT2450.09.6969584
GTATCAA36600.09.6944271
GTATTAG1804.090907E-99.5124941
TACTATA900.00111053819.5032172
AAACCGT1002.775076E-49.4935646
TAGCACA3750.09.3763124
TATACAG3350.09.3563295
Produced by FastQC (version 0.11.5)