##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921183_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2412094
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.15486958634282	31.0	30.0	33.0	25.0	34.0
2	29.450855978249603	31.0	30.0	34.0	25.0	34.0
3	29.614976033272335	31.0	30.0	34.0	25.0	34.0
4	33.26277209760482	35.0	35.0	37.0	28.0	37.0
5	32.71856818183703	35.0	33.0	37.0	26.0	37.0
6	32.654926383465984	35.0	33.0	37.0	25.0	37.0
7	32.49761659371484	35.0	33.0	37.0	24.0	37.0
8	32.443648547693414	36.0	33.0	37.0	23.0	37.0
9	33.782341401288676	38.0	34.0	39.0	23.0	39.0
10	33.64918406994089	38.0	34.0	39.0	22.0	39.0
11	33.6742726444326	38.0	34.0	39.0	22.0	39.0
12	33.52940971620509	38.0	34.0	39.0	19.0	39.0
13	33.49753367820657	38.0	34.0	39.0	19.0	39.0
14	34.42809525665252	39.0	34.0	41.0	18.0	41.0
15	34.41032895069595	39.0	34.0	41.0	17.0	41.0
16	34.37732360347482	39.0	34.0	41.0	17.0	41.0
17	34.35100083164255	39.0	34.0	41.0	17.0	41.0
18	34.30500013681059	39.0	34.0	41.0	17.0	41.0
19	34.335770496506356	39.0	34.0	41.0	18.0	41.0
20	34.27252710715254	39.0	34.0	41.0	18.0	41.0
21	34.19954612050774	39.0	34.0	41.0	16.0	41.0
22	34.09783656855827	39.0	34.0	41.0	11.0	41.0
23	33.96489398837691	39.0	34.0	41.0	10.0	41.0
24	33.88226951354301	39.0	34.0	41.0	2.0	41.0
25	33.743920842222565	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	97674.0
3	4.0
4	1610.0
5	13106.0
6	14887.0
7	11013.0
8	8497.0
9	7289.0
10	6291.0
11	5727.0
12	5437.0
13	5101.0
14	4926.0
15	4889.0
16	4959.0
17	5322.0
18	5841.0
19	6616.0
20	8338.0
21	10970.0
22	13662.0
23	16552.0
24	19194.0
25	21702.0
26	24916.0
27	27469.0
28	31419.0
29	37543.0
30	47888.0
31	60483.0
32	76312.0
33	98197.0
34	128036.0
35	168316.0
36	229621.0
37	347556.0
38	834731.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.12953954865327	18.91718946984894	9.185165696586308	31.76810528491148
2	14.672562512074572	20.720046565349442	34.74433417603128	29.863056746544704
3	17.61111304656537	23.47454407609985	28.252931798462804	30.661411078871975
4	13.386016537509718	16.178328677820538	32.72786778620204	37.707786998467704
5	14.653725833944877	37.439542081697994	32.40867997117684	15.498052113180293
6	34.393391307953024	34.879673296779615	16.03395557582479	14.692979819442572
7	29.957574644454954	31.85989558047447	19.17175822066409	19.010771554406485
8	27.828383817989067	34.73339615843701	18.39437774747542	19.043842276098502
9	27.007063230778222	15.480485840164606	18.60398686770569	38.90846406135148
10	16.89377375026844	28.437181550513706	30.75058103427833	23.918463664939523
11	35.70178864458203	22.404616263556214	21.549348796058364	20.34424629580339
12	23.58309246278322	24.77542241480811	28.394409149930098	23.24707597247857
13	28.755917303276874	20.891055284548802	25.07423318631804	25.27879422585628
14	22.690287276793864	20.759830029832717	25.28202537274305	31.26785732063037
15	24.995946639110723	27.797389179454623	22.3238384477556	24.882825733679052
16	25.009048511214143	26.96485363385308	23.961494179723957	24.064603675208822
17	23.382501887404164	27.208304329566264	25.524586419064672	23.884607363964903
18	23.639528124862665	26.47361477681951	26.3022627751147	23.584594323203127
19	24.70492244380477	26.08295297706088	25.990747070990672	23.221377508143682
20	24.804790661460533	25.51556243589414	25.993003392169072	23.686643510476255
21	25.11791259873729	25.299549411349133	25.75693752290672	23.82560046700686
22	24.935935041594025	25.15073559134413	25.441116415596156	24.47221295146569
23	24.587208757567392	25.037251336446108	25.79388671779449	24.58165318819201
24	24.35405077875433	25.55355674746983	25.731956933540022	24.360435540235812
25	24.309627896527136	25.17606840162408	25.952683810261824	24.561619891586965
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1168.0
1	1168.0
2	1219.5
3	1271.0
4	1271.0
5	1271.0
6	1596.5
7	1922.0
8	1922.0
9	1922.0
10	2522.0
11	3122.0
12	3122.0
13	3122.0
14	4273.0
15	5424.0
16	5424.0
17	5424.0
18	10418.5
19	15413.0
20	15413.0
21	15413.0
22	24568.0
23	33723.0
24	33723.0
25	33723.0
26	50169.5
27	66616.0
28	66616.0
29	66616.0
30	86888.5
31	107161.0
32	107161.0
33	107161.0
34	130569.5
35	153978.0
36	153978.0
37	153978.0
38	181598.0
39	209218.0
40	209218.0
41	209218.0
42	236020.5
43	262823.0
44	262823.0
45	262823.0
46	290443.0
47	318063.0
48	318063.0
49	318063.0
50	328519.5
51	338976.0
52	338976.0
53	338976.0
54	322263.0
55	305550.0
56	305550.0
57	305550.0
58	272324.0
59	239098.0
60	239098.0
61	239098.0
62	204236.5
63	169375.0
64	169375.0
65	169375.0
66	133686.5
67	97998.0
68	97998.0
69	97998.0
70	72996.5
71	47995.0
72	47995.0
73	47995.0
74	34237.5
75	20480.0
76	20480.0
77	20480.0
78	14217.5
79	7955.0
80	7955.0
81	7955.0
82	5563.0
83	3171.0
84	3171.0
85	3171.0
86	2163.5
87	1156.0
88	1156.0
89	1156.0
90	773.5
91	391.0
92	391.0
93	391.0
94	217.5
95	44.0
96	44.0
97	44.0
98	23.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09651365162385878
2	0.0
3	0.03921903541072611
4	0.05522172850643466
5	0.0635547370873606
6	0.0049334727419412345
7	0.003855571134458276
8	0.004435979692333715
9	0.0010364438533489988
10	6.633240661433592E-4
11	0.004145775413395995
12	4.145775413395995E-4
13	0.004145775413395995
14	0.05464131994855922
15	0.021350743378989376
16	0.003938486642726196
17	0.0021143454608319578
18	0.004145775413395995
19	0.0041872331675299555
20	0.0028191272811092767
21	0.005721170070486474
22	0.0036897401179224357
23	0.004145775413395995
24	0.0041872331675299555
25	0.16918909462069057
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2412094.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.52368069417184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.780017633534825	21.328736020604616
2	13.128689309163615	9.06501355285104
3	7.212632412120086	7.470198550814048
4	4.424094111434312	6.109440498564962
5	2.935103103541793	5.066528117557483
6	2.0903972688484282	4.330092470021522
7	1.515896890333559	3.6634038145014673
8	1.1609524732673073	3.206428199055167
9	0.8923040315277997	2.7725057519929193
>10	4.604921414241951	27.08892436566595
>50	0.18362700094666845	4.3105528579367896
>100	0.06772673753844695	3.832053704597424
>500	0.00206131431729793	0.5030887308470738
>1k	0.0014550454004455316	1.0248475876683014
>5k	1.2125378337046099E-4	0.2281857773212937
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5451	0.22598621778421568	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3772	0.15637864859329695	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3643	0.1510305983100161	No Hit
CTTATACACATCTCCGAGCCCACGA	2841	0.11778147949458022	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2685	0.11131406984968246	No Hit
GGTATCAACGCAGAGTACATGGGGG	2419	0.10028630725004911	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	4.145775413395995E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1945	0.0	13.205005	1
GTACTAG	85	3.8883027E-6	12.3102865	1
TTATACA	540	0.0	11.967013	2
CTTATAC	535	0.0	11.557236	1
TATACAC	570	0.0	11.170446	3
GAGCGCG	60	0.0058715227	11.084785	11
AAGACCG	185	5.456968E-12	10.781618	5
ACACCGT	100	2.417894E-5	10.442921	6
GTATAGA	150	2.3970642E-8	10.14666	1
TACGCTG	85	6.607942E-4	10.056804	5
GTATATA	250	0.0	9.892994	1
ACTATAC	125	2.5600948E-6	9.883345	3
CAATACC	135	6.418177E-7	9.854983	4
TTAGGGT	245	0.0	9.696958	4
GTATCAA	3660	0.0	9.694427	1
GTATTAG	180	4.090907E-9	9.512494	1
TACTATA	90	0.0011105381	9.503217	2
AAACCGT	100	2.775076E-4	9.493564	6
TAGCACA	375	0.0	9.376312	4
TATACAG	335	0.0	9.356329	5
>>END_MODULE