FastQCFastQC Report
Mon 15 Aug 2016
SRR2921182_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921182_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2255352
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27410.1215331354041409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12500.013.4545141
ATAAACG550.00308020412.0851244
ATCGTAT803.7771853E-410.68594112
GTAAGAC803.7872614E-410.682863
GTATCAA28900.010.652511
GTATTAA2400.010.293591
GTGTTAG1856.002665E-1110.272191
GTCTAGG2054.1836756E-119.7335251
GTATAAT2800.09.5017761
AGGATCG1201.7101296E-59.4956655
CTAGGTA1002.7693517E-49.4954554
TAGCACT2151.07320375E-109.2746314
GTCTAAG1951.6771082E-99.2581411
CCAATAC1356.886814E-69.14417653
GTAGCGT1151.1161446E-49.0830113
ACTATAC1054.5129927E-49.0436923
GGTCGTT950.00183060468.9964927
GTCCTAG2750.08.9834961
GTATAAC1701.6804916E-78.9428471
TATACTG2356.730261E-118.8895595
Produced by FastQC (version 0.11.5)