##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921182_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2255352
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.758735221819034	31.0	27.0	31.0	10.0	34.0
2	26.550339813918182	31.0	26.0	31.0	7.0	34.0
3	26.281075858668626	31.0	26.0	31.0	7.0	34.0
4	30.19269807994495	35.0	30.0	37.0	10.0	37.0
5	29.760352707692636	35.0	30.0	37.0	7.0	37.0
6	30.200099141952123	35.0	30.0	37.0	2.0	37.0
7	30.10485325572239	35.0	30.0	37.0	2.0	37.0
8	29.836606436600583	35.0	30.0	37.0	2.0	37.0
9	31.365669305722566	37.0	31.0	39.0	2.0	39.0
10	30.745535951815945	37.0	30.0	39.0	2.0	39.0
11	31.17178382797896	37.0	30.0	39.0	2.0	39.0
12	30.877913514165417	37.0	30.0	39.0	2.0	39.0
13	30.874864766120766	37.0	30.0	39.0	2.0	39.0
14	31.95142044346071	38.0	31.0	40.0	2.0	41.0
15	31.59084612956204	38.0	30.0	40.0	2.0	41.0
16	31.50928059123365	38.0	30.0	40.0	2.0	41.0
17	31.826900634579435	38.0	31.0	40.0	2.0	41.0
18	31.87913061907853	38.0	31.0	40.0	2.0	41.0
19	31.58296975372359	38.0	30.0	40.0	2.0	41.0
20	31.442692315877967	38.0	30.0	40.0	2.0	41.0
21	31.38998347042945	38.0	30.0	40.0	2.0	41.0
22	30.623640566971364	38.0	28.0	40.0	2.0	41.0
23	30.722273951028487	38.0	29.0	40.0	2.0	41.0
24	30.35236849946261	37.0	28.0	40.0	2.0	41.0
25	29.8605725403396	37.0	27.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	206115.0
3	7.0
4	1765.0
5	9521.0
6	10952.0
7	9584.0
8	8127.0
9	7527.0
10	7075.0
11	6761.0
12	6322.0
13	6328.0
14	6291.0
15	6545.0
16	6862.0
17	7623.0
18	8638.0
19	10846.0
20	13258.0
21	16742.0
22	20168.0
23	23392.0
24	27564.0
25	31026.0
26	34198.0
27	38462.0
28	42358.0
29	49848.0
30	59384.0
31	69647.0
32	87096.0
33	110213.0
34	139728.0
35	179914.0
36	235818.0
37	308144.0
38	441503.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.31162052573079	17.852235460260605	12.638748805782276	25.197395208226332
2	15.940075770346645	19.578117098013468	38.79965220168753	25.682154929952354
3	19.145708409348313	23.265505947128165	29.670808208110017	27.917977435413505
4	13.701225736475084	15.47225131632976	34.715808461936184	36.110714485258974
5	14.064696361661086	36.51393477152296	34.01662860563599	15.404740261179967
6	34.28924496327374	33.85423179005859	17.074987562817746	14.781535683849931
7	29.526364821069333	31.470443084364145	20.467139250968515	18.536052843598007
8	26.535551147201673	34.1813157724379	20.36350592423051	18.919627156129923
9	27.03394313530474	15.765207100171914	19.01133063706178	38.18951912746157
10	16.10788338690375	27.849094150766625	32.64808425712559	23.39493820520404
11	35.41228132800212	22.696125795790696	22.175538889482592	19.716053986724592
12	23.65961065952685	24.688324087077252	28.991059119427693	22.661006133968208
13	28.444639356423902	21.387943767795388	25.291543929026417	24.875872946754292
14	22.851160012399006	20.845012835812028	25.70067037129177	30.603156780497194
15	24.683798006743515	27.909182495242113	22.83926420847072	24.56775528954365
16	24.253219306703663	27.145783122911567	24.66764655529526	23.933351015089517
17	23.205942180307524	27.04723742374229	25.717246108494646	24.029574287455546
18	23.151592990566545	27.058421258737287	26.452792584153162	23.337193166543006
19	24.53185629964035	26.19697823030905	26.529089078790406	22.742076391260195
20	24.90318151763664	25.683716091358157	25.958262398153785	23.45483999285142
21	25.29288213046471	25.39918716782448	25.6839977435126	23.623932958198214
22	24.38912020716658	24.818884439891985	26.98699444392712	23.805000909014318
23	24.61739111539398	25.178682373538734	25.987470393294128	24.21645611777316
24	24.293980727009725	25.509772854359923	26.147401880116522	24.04884453851383
25	23.807890436930137	25.154398256627186	26.845027984127185	24.192683322315492
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1194.0
1	1194.0
2	1285.0
3	1376.0
4	1376.0
5	1376.0
6	1703.5
7	2031.0
8	2031.0
9	2031.0
10	2664.0
11	3297.0
12	3297.0
13	3297.0
14	4932.0
15	6567.0
16	6567.0
17	6567.0
18	11440.5
19	16314.0
20	16314.0
21	16314.0
22	25200.5
23	34087.0
24	34087.0
25	34087.0
26	50808.5
27	67530.0
28	67530.0
29	67530.0
30	88303.0
31	109076.0
32	109076.0
33	109076.0
34	132983.0
35	156890.0
36	156890.0
37	156890.0
38	183812.0
39	210734.0
40	210734.0
41	210734.0
42	235601.5
43	260469.0
44	260469.0
45	260469.0
46	281498.0
47	302527.0
48	302527.0
49	302527.0
50	307100.0
51	311673.0
52	311673.0
53	311673.0
54	293652.0
55	275631.0
56	275631.0
57	275631.0
58	244226.0
59	212821.0
60	212821.0
61	212821.0
62	178001.0
63	143181.0
64	143181.0
65	143181.0
66	111574.5
67	79968.0
68	79968.0
69	79968.0
70	58850.0
71	37732.0
72	37732.0
73	37732.0
74	26272.0
75	14812.0
76	14812.0
77	14812.0
78	9995.5
79	5179.0
80	5179.0
81	5179.0
82	3419.5
83	1660.0
84	1660.0
85	1660.0
86	1079.5
87	499.0
88	499.0
89	499.0
90	293.0
91	87.0
92	87.0
93	87.0
94	48.5
95	10.0
96	10.0
97	10.0
98	8.5
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03272216487714556
2	0.05196528080760786
3	0.013922438714666271
4	0.009665896942029448
5	0.008956473313256644
6	7.98101582369404E-4
7	0.00412352484224192
8	0.0016848811183354086
9	0.006163117774963731
10	0.0028820334918895144
11	0.009089490243651546
12	0.009089490243651546
13	0.009000812290054944
14	0.014764879273833974
15	0.006562168566148432
16	0.029884470362054348
17	0.03662399483539598
18	0.022391183283141613
19	0.016937489136950683
20	0.015917692670589782
21	0.023056267935116115
22	0.007182914241324636
23	0.016538438345765984
24	0.012370574526725762
25	0.46591396819653874
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2255352.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.10391030600708
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.10477132177535	37.359396765691656
2	12.039835971759892	12.305653951997103
3	5.4019695642834265	8.281853042667612
4	2.9110574465542336	5.950656745773662
5	1.7916650965685423	4.578054619672115
6	1.115654443935013	3.420858278121186
7	0.7635851464905384	2.7315530786076225
8	0.5525467421538318	2.258983932072468
9	0.4013842738797379	1.8461075337532713
>10	1.8184124337160754	15.73546815911319
>50	0.07285965738122449	2.4850768887073644
>100	0.024735704169079255	2.256720349715888
>500	9.84951215485214E-4	0.3676732623869171
>1k	5.372461175373809E-4	0.4219433917199405
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2741	0.1215331354041409	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7735590719320088E-4
2	0.0	0.0	0.0	0.0	1.7735590719320088E-4
3	0.0	0.0	0.0	0.0	1.7735590719320088E-4
4	0.0	0.0	0.0	0.0	1.7735590719320088E-4
5	0.0	0.0	0.0	0.0	2.216948839915011E-4
6	0.0	4.433897679830022E-5	0.0	0.0	3.103728375881015E-4
7	0.0	4.433897679830022E-5	0.0	0.0	3.103728375881015E-4
8	0.0	4.433897679830022E-5	0.0	0.0	3.103728375881015E-4
9	0.0	4.433897679830022E-5	0.0	0.0	3.103728375881015E-4
10	0.0	4.433897679830022E-5	0.0	0.0	3.5471181438640175E-4
11	0.0	4.433897679830022E-5	0.0	0.0	3.5471181438640175E-4
12	0.0	4.433897679830022E-5	0.0	0.0	6.20745675176203E-4
13	0.0	4.433897679830022E-5	0.0	0.0	7.537626055711037E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1250	0.0	13.454514	1
ATAAACG	55	0.003080204	12.085124	4
ATCGTAT	80	3.7771853E-4	10.685941	12
GTAAGAC	80	3.7872614E-4	10.68286	3
GTATCAA	2890	0.0	10.65251	1
GTATTAA	240	0.0	10.29359	1
GTGTTAG	185	6.002665E-11	10.27219	1
GTCTAGG	205	4.1836756E-11	9.733525	1
GTATAAT	280	0.0	9.501776	1
AGGATCG	120	1.7101296E-5	9.495665	5
CTAGGTA	100	2.7693517E-4	9.495455	4
TAGCACT	215	1.07320375E-10	9.274631	4
GTCTAAG	195	1.6771082E-9	9.258141	1
CCAATAC	135	6.886814E-6	9.1441765	3
GTAGCGT	115	1.1161446E-4	9.083011	3
ACTATAC	105	4.5129927E-4	9.043692	3
GGTCGTT	95	0.0018306046	8.996492	7
GTCCTAG	275	0.0	8.983496	1
GTATAAC	170	1.6804916E-7	8.942847	1
TATACTG	235	6.730261E-11	8.889559	5
>>END_MODULE