FastQCFastQC Report
Mon 15 Aug 2016
SRR2921182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2255352
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49020.21734966426526767No Hit
TATCAACGCAGAGTACTTTTTTTTT34020.15084119906781734No Hit
GGTATCAACGCAGAGTACTTTTTTT32790.14538750492162641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15950.013.8953321
GCCGCGT701.0933445E-412.2118628
CGTATTG550.00306762612.09172814
TGTGCGA700.001491010310.85787910
TCTTATA1553.6307028E-910.4253812
TTATACG750.002635687610.1391952
TAGAACA2550.010.0646414
CGATTCG856.5912935E-410.05950459
TACTAGC951.6350005E-410.0057842
TTAGCAC1356.394548E-79.8575513
GTATCAA31250.09.8012081
CTTATAC2350.09.7144251
TAGACAG2750.09.6720955
GTACTAA1201.6756023E-59.5120411
TCTACAC3200.09.5054943
TACACCG1304.2434276E-69.4993785
CCTCTAG3350.09.370071
TAGGACA3250.09.3592564
AATAATA2950.09.3443852
CTAGACA2850.09.3387324
Produced by FastQC (version 0.11.5)