##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921182_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2255352
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.508321539165504	31.0	30.0	33.0	26.0	34.0
2	29.794510125248742	31.0	30.0	34.0	26.0	34.0
3	29.94675110581408	31.0	30.0	34.0	26.0	34.0
4	33.62150919235667	35.0	35.0	37.0	30.0	37.0
5	33.131156466928445	35.0	35.0	37.0	27.0	37.0
6	33.095441421117414	36.0	35.0	37.0	26.0	37.0
7	32.95518482259089	36.0	35.0	37.0	26.0	37.0
8	32.909861963897434	36.0	35.0	37.0	26.0	37.0
9	34.29329967118215	39.0	35.0	39.0	26.0	39.0
10	34.16148122333011	38.0	35.0	39.0	25.0	39.0
11	34.192947708384324	38.0	35.0	39.0	26.0	39.0
12	34.05669713641152	38.0	35.0	39.0	25.0	39.0
13	34.02747464697307	38.0	35.0	39.0	25.0	39.0
14	34.99537101082226	39.0	35.0	41.0	25.0	41.0
15	34.98540272205847	39.0	35.0	41.0	25.0	41.0
16	34.96077818451399	39.0	35.0	41.0	25.0	41.0
17	34.94249678099028	39.0	35.0	41.0	25.0	41.0
18	34.89794320354428	39.0	35.0	41.0	24.0	41.0
19	34.93634519134929	39.0	35.0	41.0	24.0	41.0
20	34.871443570671005	39.0	35.0	41.0	23.0	41.0
21	34.805890167033795	39.0	35.0	41.0	23.0	41.0
22	34.71210658025887	39.0	35.0	41.0	22.0	41.0
23	34.585402189990745	39.0	34.0	41.0	19.0	41.0
24	34.50729154473448	39.0	34.0	41.0	18.0	41.0
25	34.367958527094665	39.0	34.0	41.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	75730.0
3	2.0
4	1206.0
5	10142.0
6	11327.0
7	8475.0
8	6873.0
9	5731.0
10	5020.0
11	4573.0
12	4372.0
13	4069.0
14	3947.0
15	4080.0
16	4098.0
17	4338.0
18	4814.0
19	5655.0
20	7040.0
21	9196.0
22	11331.0
23	14041.0
24	16371.0
25	18991.0
26	21734.0
27	24617.0
28	28178.0
29	34052.0
30	43256.0
31	55287.0
32	70543.0
33	91730.0
34	120970.0
35	161105.0
36	219342.0
37	335127.0
38	807989.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.031467336549596	19.17039851229179	9.394886224940416	31.403247926218192
2	14.903039525537476	20.901083289881136	34.830837935719124	29.365039248862264
3	17.71698162586458	23.563410350959604	28.27650938002208	30.44309864315374
4	13.597843928840778	16.141076261035447	32.9008473448383	37.36023246528548
5	14.679636705215007	37.44989921050074	32.51926714519678	15.351196939087469
6	34.53362144386656	34.76810664287523	16.04848983638804	14.64978207687017
7	30.074927258747795	31.819475766366008	19.306118069184226	18.799478905701974
8	27.86548750734979	34.69447481914857	18.441879666507923	18.99815800699372
9	27.162766073955112	15.650981381529444	18.656291886681437	38.529960657834
10	16.874151184583848	28.476568371458256	30.83505036249379	23.81423008146411
11	35.87151244257613	22.495477039323152	21.771404246261906	19.861606271838806
12	23.85776043629592	24.737047464916753	28.415811293147403	22.98938080563992
13	28.726547302693163	21.065203520171547	25.05171310610753	25.15653607102776
14	23.096358756920527	20.649933916981627	25.42818645925901	30.825520866838836
15	24.865702447268838	27.907104632129148	22.43843466684051	24.788758253761507
16	24.852019579664216	26.954895736381346	23.939726773315147	24.25335791063929
17	23.343509250329774	26.958198929200893	25.684546573110307	24.01374524735903
18	23.304071434684417	26.753197457731208	26.43982598061061	23.502905126973758
19	24.777246754813127	25.988011631856526	26.09265931945303	23.142082293877312
20	24.959063113970153	25.618442948413538	25.868609112486535	23.553884825129774
21	25.119334599849935	25.101818001209754	25.81769256285133	23.961154836088983
22	25.180344820094152	24.829742695197854	25.594176821062554	24.39573566364544
23	24.670593895428112	25.036282893497013	25.880910519932865	24.412212691142006
24	24.365970908249196	25.495079908529	25.921394715452383	24.21755446776942
25	24.308831498805112	25.179321802013092	25.945337277791104	24.566509421390688
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	801.0
1	801.0
2	861.5
3	922.0
4	922.0
5	922.0
6	1215.0
7	1508.0
8	1508.0
9	1508.0
10	2047.5
11	2587.0
12	2587.0
13	2587.0
14	3572.0
15	4557.0
16	4557.0
17	4557.0
18	8946.5
19	13336.0
20	13336.0
21	13336.0
22	21597.5
23	29859.0
24	29859.0
25	29859.0
26	46281.5
27	62704.0
28	62704.0
29	62704.0
30	81124.0
31	99544.0
32	99544.0
33	99544.0
34	122226.0
35	144908.0
36	144908.0
37	144908.0
38	171673.0
39	198438.0
40	198438.0
41	198438.0
42	224366.0
43	250294.0
44	250294.0
45	250294.0
46	275070.0
47	299846.0
48	299846.0
49	299846.0
50	309955.5
51	320065.0
52	320065.0
53	320065.0
54	303785.0
55	287505.0
56	287505.0
57	287505.0
58	257451.5
59	227398.0
60	227398.0
61	227398.0
62	191638.0
63	155878.0
64	155878.0
65	155878.0
66	122313.0
67	88748.0
68	88748.0
69	88748.0
70	65185.0
71	41622.0
72	41622.0
73	41622.0
74	28952.0
75	16282.0
76	16282.0
77	16282.0
78	11073.0
79	5864.0
80	5864.0
81	5864.0
82	3903.5
83	1943.0
84	1943.0
85	1943.0
86	1257.5
87	572.0
88	572.0
89	572.0
90	362.0
91	152.0
92	152.0
93	152.0
94	83.5
95	15.0
96	15.0
97	15.0
98	9.5
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09852120644582309
2	0.0
3	0.041368265352814106
4	0.05551239895147188
5	0.07293761683320386
6	0.008823456382861744
7	0.0043452197262334215
8	0.00824704968448384
9	4.8772874478130243E-4
10	1.3301693039490068E-4
11	0.008513083545273643
12	8.867795359660045E-5
13	0.005365016192594326
14	0.060700059236873
15	0.020883658071999406
16	0.006872541403736534
17	0.001197152373554106
18	0.008912134336458344
19	0.007360270148517836
20	0.0022612878167133114
21	0.015429963925808478
22	0.00394616893504872
23	0.0069612193573331356
24	0.009355524104441347
25	0.1814350930586445
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2255352.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.68100824184219
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.783977405334	23.280825618241828
2	15.041309736625074	11.335434323077413
3	7.546140807237303	8.530385818548316
4	4.3435667286391295	6.546798948033704
5	2.75394346007527	5.188568310833183
6	1.8415443935932079	4.163474968362237
7	1.3290835080487913	3.5056844634618125
8	0.9686587744053476	2.9200031409520535
9	0.7201277780459573	2.4421626665756304
>10	3.4485088506323494	22.39993044587111
>50	0.16273541753219073	4.146132127305863
>100	0.05624403513949605	3.703759909027938
>500	0.0027331259404595855	0.7028634844632167
>1k	0.001425978751544108	1.1339757752456354
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4902	0.21734966426526767	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3402	0.15084119906781734	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3279	0.14538750492162641	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3301693039490065E-4
2	0.0	0.0	0.0	0.0	1.3301693039490065E-4
3	0.0	0.0	0.0	0.0	1.3301693039490065E-4
4	0.0	0.0	0.0	4.433897679830022E-5	1.3301693039490065E-4
5	0.0	0.0	0.0	4.433897679830022E-5	1.3301693039490065E-4
6	0.0	0.0	0.0	4.433897679830022E-5	1.3301693039490065E-4
7	0.0	0.0	0.0	4.433897679830022E-5	1.3301693039490065E-4
8	0.0	0.0	0.0	4.433897679830022E-5	1.3301693039490065E-4
9	0.0	0.0	0.0	4.433897679830022E-5	1.3301693039490065E-4
10	0.0	0.0	0.0	4.433897679830022E-5	1.7735590719320088E-4
11	0.0	0.0	0.0	4.433897679830022E-5	1.7735590719320088E-4
12	0.0	0.0	0.0	4.433897679830022E-5	7.094236287728035E-4
13	0.0	0.0	0.0	4.433897679830022E-5	8.867795359660044E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1595	0.0	13.895332	1
GCCGCGT	70	1.0933445E-4	12.211862	8
CGTATTG	55	0.003067626	12.091728	14
TGTGCGA	70	0.0014910103	10.857879	10
TCTTATA	155	3.6307028E-9	10.425381	2
TTATACG	75	0.0026356876	10.139195	2
TAGAACA	255	0.0	10.064641	4
CGATTCG	85	6.5912935E-4	10.0595045	9
TACTAGC	95	1.6350005E-4	10.005784	2
TTAGCAC	135	6.394548E-7	9.857551	3
GTATCAA	3125	0.0	9.801208	1
CTTATAC	235	0.0	9.714425	1
TAGACAG	275	0.0	9.672095	5
GTACTAA	120	1.6756023E-5	9.512041	1
TCTACAC	320	0.0	9.505494	3
TACACCG	130	4.2434276E-6	9.499378	5
CCTCTAG	335	0.0	9.37007	1
TAGGACA	325	0.0	9.359256	4
AATAATA	295	0.0	9.344385	2
CTAGACA	285	0.0	9.338732	4
>>END_MODULE