FastQCFastQC Report
Mon 15 Aug 2016
SRR2921181_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921181_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2904254
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33830.11648430199286976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13450.012.1522251
TCGCGCA951.3656769E-510.9967518
TAGACCG700.001497480610.852445
GTATCAA30450.010.7978771
ACACCGT1605.493348E-1010.6832386
CCATGCG1252.2541099E-710.6359419
CGTGCGC2250.010.55261810
GTCCTAT2800.010.5209091
TGGACCG2001.8189894E-1210.4454745
CGTTCGC1201.5283658E-610.28986615
GTATAAT3600.09.5027571
GCGTGCG1901.0604708E-99.4963759
CTTACAC3250.09.3501163
GTCTTAA2900.09.1750761
ACACCCG1252.76969E-59.1160495
GTACTAG1804.2022293E-88.9748261
TCTATAC2356.730261E-118.8900713
TACACAG7700.08.879275
ACGAAGG1502.7097612E-68.86511516
CTACACT3450.08.8079294
Produced by FastQC (version 0.11.5)