##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921181_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2904254
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.29022599263012	31.0	28.0	31.0	10.0	34.0
2	27.097808249553932	31.0	27.0	31.0	10.0	34.0
3	26.867523639461286	31.0	26.0	31.0	10.0	34.0
4	30.78870925201446	35.0	31.0	37.0	12.0	37.0
5	30.47201966494666	35.0	30.0	37.0	10.0	37.0
6	30.833402656930144	35.0	32.0	37.0	10.0	37.0
7	30.779118837401963	35.0	32.0	37.0	10.0	37.0
8	30.403604849989016	35.0	31.0	37.0	10.0	37.0
9	32.04908145086483	37.0	32.0	39.0	10.0	39.0
10	31.41589062113713	37.0	30.0	39.0	8.0	39.0
11	31.866871836967427	37.0	32.0	39.0	7.0	39.0
12	31.558160546563766	37.0	31.0	39.0	2.0	39.0
13	31.572780479944246	37.0	31.0	39.0	2.0	39.0
14	32.683109672914284	38.0	32.0	40.0	2.0	41.0
15	32.342140873353365	38.0	31.0	40.0	2.0	41.0
16	32.261791496198335	38.0	31.0	40.0	2.0	41.0
17	32.57608907485365	38.0	32.0	40.0	2.0	41.0
18	32.628932249038826	38.0	32.0	40.0	2.0	41.0
19	32.34991188787207	38.0	31.0	40.0	2.0	41.0
20	32.21722858951042	38.0	31.0	40.0	2.0	41.0
21	32.17464415991164	38.0	31.0	40.0	2.0	41.0
22	31.41385051032038	38.0	30.0	40.0	2.0	41.0
23	31.5165935899546	38.0	30.0	40.0	2.0	41.0
24	31.15646462051873	38.0	30.0	40.0	2.0	41.0
25	30.66719921880111	38.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	224926.0
3	11.0
4	1894.0
5	10694.0
6	12826.0
7	11327.0
8	9656.0
9	8857.0
10	8336.0
11	7973.0
12	7827.0
13	7505.0
14	7410.0
15	7811.0
16	8354.0
17	8949.0
18	10531.0
19	12879.0
20	16074.0
21	20285.0
22	24837.0
23	29219.0
24	33344.0
25	38227.0
26	42293.0
27	47956.0
28	53647.0
29	62795.0
30	74072.0
31	88523.0
32	110506.0
33	139492.0
34	180561.0
35	232401.0
36	308304.0
37	409821.0
38	624131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.24811833253194	17.916002474446106	12.422621893337043	25.41325729968491
2	15.773555967654199	19.768172120504747	39.0559044384356	25.40236747340546
3	18.902595671791154	23.104213257369395	29.920649734110143	28.0725413367293
4	13.399466039293253	15.696502602137288	35.76589752128043	35.138133837289026
5	13.770943641562763	36.46356178270955	34.634970440608484	15.130524135119202
6	33.70375879579702	34.464580604553085	17.367438093703385	14.464222505946504
7	29.230550974934776	31.93861321567154	20.399959643860512	18.43087616553317
8	26.036775754804424	34.40793940904815	20.614070124743648	18.941214711403774
9	26.49512992491207	16.087156541423703	19.374448617143848	38.04326491652038
10	15.475879755853503	28.42211064274051	33.154738826193345	22.947270775212644
11	35.60525710444649	22.81911296176664	22.232051725301623	19.34357820848525
12	23.19551791405318	24.871582521379956	29.492339028341892	22.44056053622497
13	28.38522951686188	21.539409821162625	25.499830408380063	24.575530253595428
14	22.45918293552264	21.063568208663792	26.03028435056013	30.446964505253444
15	24.32200789723879	28.24180550423839	23.15047280473187	24.285713793790954
16	23.82370770417723	27.556830706491787	25.070022318968366	23.549439270362615
17	22.90613635095411	27.41985707819778	25.99263690242862	23.68136966841949
18	22.807544414615624	27.333986544313017	26.774061898915313	23.084407142156042
19	23.98754033549489	26.594564996538985	26.829500959098002	22.588393708868125
20	24.35742731238696	26.269062978253295	26.38088212808338	22.99262758127637
21	24.953738478530624	25.846491993906877	26.00963424382344	23.190135283739053
22	23.74295558584251	25.421631817921998	27.396650360837917	23.438762235397583
23	24.12581488330768	25.745560112818076	26.216909625007318	23.91171537886693
24	23.717781612476767	25.904845667522874	26.533518969190666	23.84385375080969
25	23.29846690341474	25.800408572056234	27.1199821487569	23.781142375772127
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1288.0
1	1288.0
2	1377.0
3	1466.0
4	1466.0
5	1466.0
6	1933.5
7	2401.0
8	2401.0
9	2401.0
10	3284.5
11	4168.0
12	4168.0
13	4168.0
14	6470.0
15	8772.0
16	8772.0
17	8772.0
18	15482.0
19	22192.0
20	22192.0
21	22192.0
22	35569.0
23	48946.0
24	48946.0
25	48946.0
26	72666.5
27	96387.0
28	96387.0
29	96387.0
30	126912.5
31	157438.0
32	157438.0
33	157438.0
34	187219.0
35	217000.0
36	217000.0
37	217000.0
38	252399.5
39	287799.0
40	287799.0
41	287799.0
42	317781.0
43	347763.0
44	347763.0
45	347763.0
46	369781.5
47	391800.0
48	391800.0
49	391800.0
50	391381.0
51	390962.0
52	390962.0
53	390962.0
54	362735.5
55	334509.0
56	334509.0
57	334509.0
58	294316.5
59	254124.0
60	254124.0
61	254124.0
62	211447.5
63	168771.0
64	168771.0
65	168771.0
66	131758.0
67	94745.0
68	94745.0
69	94745.0
70	69909.0
71	45073.0
72	45073.0
73	45073.0
74	31810.0
75	18547.0
76	18547.0
77	18547.0
78	12601.5
79	6656.0
80	6656.0
81	6656.0
82	4532.0
83	2408.0
84	2408.0
85	2408.0
86	1614.0
87	820.0
88	820.0
89	820.0
90	504.0
91	188.0
92	188.0
93	188.0
94	105.5
95	23.0
96	23.0
97	23.0
98	15.5
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03367474057021184
2	0.051545078357471484
3	0.014461545030152321
4	0.010157513771178416
5	0.009296707519383635
6	8.952385018665723E-4
7	0.0038219797579688275
8	0.0019970705041638917
9	0.006610992013783919
10	0.0033054960068919594
11	0.01026081052139379
12	0.008573630267876019
13	0.007540662765722282
14	0.01528791903187531
15	0.006920882264430039
16	0.030782431564181367
17	0.03883957808098052
18	0.022346530296592518
19	0.016665209034746963
20	0.015769970532880387
21	0.02248425929687968
22	0.006197805012922424
23	0.015976564033311134
24	0.012430042275916639
25	0.4716873937334682
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2904254.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.381403761189354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.79365732190963	29.843654244906613
2	12.102584305511435	10.5005419262245
3	5.89665441712029	7.674154383278879
4	3.5218351008733473	6.111286019652629
5	2.306358620694034	5.002653727121382
6	1.6321528230005333	4.24830483687307
7	1.14672559220862	3.482259614322378
8	0.8359524157259921	2.90118314174007
9	0.662703312519069	2.587409997684064
>10	2.9655589336925043	21.267423347861072
>50	0.09774517070867694	2.8572165926275432
>100	0.03608832881547624	2.7025641703315775
>500	0.0014877429152529615	0.44163545133889
>1k	4.959143050656321E-4	0.3797125460373821
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3383	0.11648430199286976	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	3.443225007179124E-5	0.0	0.0	0.0
5	0.0	6.886450014358248E-5	0.0	0.0	0.0
6	0.0	6.886450014358248E-5	0.0	0.0	0.0
7	0.0	6.886450014358248E-5	0.0	0.0	0.0
8	0.0	6.886450014358248E-5	0.0	0.0	0.0
9	0.0	6.886450014358248E-5	0.0	3.443225007179124E-5	0.0
10	0.0	6.886450014358248E-5	0.0	3.443225007179124E-5	0.0
11	0.0	6.886450014358248E-5	0.0	3.443225007179124E-5	0.0
12	0.0	6.886450014358248E-5	0.0	3.443225007179124E-5	0.0
13	0.0	1.7216125035895622E-4	0.0	3.443225007179124E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1345	0.0	12.152225	1
TCGCGCA	95	1.3656769E-5	10.99675	18
TAGACCG	70	0.0014974806	10.85244	5
GTATCAA	3045	0.0	10.797877	1
ACACCGT	160	5.493348E-10	10.683238	6
CCATGCG	125	2.2541099E-7	10.635941	9
CGTGCGC	225	0.0	10.552618	10
GTCCTAT	280	0.0	10.520909	1
TGGACCG	200	1.8189894E-12	10.445474	5
CGTTCGC	120	1.5283658E-6	10.289866	15
GTATAAT	360	0.0	9.502757	1
GCGTGCG	190	1.0604708E-9	9.496375	9
CTTACAC	325	0.0	9.350116	3
GTCTTAA	290	0.0	9.175076	1
ACACCCG	125	2.76969E-5	9.116049	5
GTACTAG	180	4.2022293E-8	8.974826	1
TCTATAC	235	6.730261E-11	8.890071	3
TACACAG	770	0.0	8.87927	5
ACGAAGG	150	2.7097612E-6	8.865115	16
CTACACT	345	0.0	8.807929	4
>>END_MODULE