FastQCFastQC Report
Mon 15 Aug 2016
SRR2921181_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921181_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2904254
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63280.21788727845429498No Hit
GGTATCAACGCAGAGTACTTTTTTT40220.13848650978874438No Hit
TATCAACGCAGAGTACTTTTTTTTT36360.12519566126103296No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTG500.001500770913.2992045
ACGACTG655.4587323E-513.1530595
GGTATCA17700.013.0625231
GTGCGAT1158.8236084E-710.73922711
GTCCTAG2400.010.7040121
GTATTAC1252.1951018E-710.6564381
GTATCAA36950.010.3000561
CCGCGGT750.002648555710.1334269
GTACGTT750.00266487810.1259237
TCTATAC2350.010.1117013
TAGGCCG856.600246E-410.0582215
TATAGAC1901.0422809E-99.5053
CCGATAC800.00449787839.5049993
CGTGCGC2303.6379788E-129.50008610
CGAGCCG800.0045171049.50008614
AACGTAC900.00112160569.49305256
TAATACC2252.7284841E-119.2937774
TGGACCG1856.710252E-99.2426895
AGGCCGT1554.3089676E-79.1868256
ATTCGCC1054.4940296E-49.04770211
Produced by FastQC (version 0.11.5)