##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921181_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2904254
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.736512715485627	31.0	30.0	33.0	26.0	34.0
2	29.971834075118775	31.0	30.0	34.0	26.0	34.0
3	30.097061414049872	31.0	30.0	34.0	26.0	34.0
4	33.79105546553435	35.0	35.0	37.0	30.0	37.0
5	33.36270932225625	35.0	35.0	37.0	28.0	37.0
6	33.33351972658039	36.0	35.0	37.0	28.0	37.0
7	33.192539977563946	36.0	35.0	37.0	27.0	37.0
8	33.13872856850675	37.0	35.0	37.0	26.0	37.0
9	34.54301311111218	39.0	35.0	39.0	27.0	39.0
10	34.4081058337184	38.0	35.0	39.0	27.0	39.0
11	34.44536187261858	38.0	35.0	39.0	27.0	39.0
12	34.31511775485202	38.0	35.0	39.0	26.0	39.0
13	34.29560258847883	38.0	35.0	39.0	26.0	39.0
14	35.26146955466017	39.0	35.0	41.0	26.0	41.0
15	35.25444640861302	39.0	35.0	41.0	26.0	41.0
16	35.23983852652006	39.0	35.0	41.0	26.0	41.0
17	35.219073469469265	39.0	35.0	41.0	25.0	41.0
18	35.1694486776983	39.0	35.0	41.0	25.0	41.0
19	35.21983924271086	39.0	35.0	41.0	25.0	41.0
20	35.15901122973404	39.0	35.0	41.0	25.0	41.0
21	35.08953004799167	39.0	35.0	41.0	25.0	41.0
22	35.00678418623165	39.0	35.0	41.0	25.0	41.0
23	34.88344304595948	39.0	35.0	41.0	24.0	41.0
24	34.80739701141842	39.0	35.0	41.0	24.0	41.0
25	34.6787536489577	39.0	35.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	85746.0
3	3.0
4	1365.0
5	11574.0
6	13152.0
7	10049.0
8	7953.0
9	6828.0
10	5996.0
11	5553.0
12	5166.0
13	4976.0
14	4797.0
15	4827.0
16	4985.0
17	5194.0
18	5649.0
19	6842.0
20	8868.0
21	11086.0
22	14326.0
23	17451.0
24	20750.0
25	23938.0
26	27581.0
27	31177.0
28	35872.0
29	44004.0
30	55539.0
31	71727.0
32	91900.0
33	118905.0
34	157095.0
35	207866.0
36	284015.0
37	428884.0
38	1062615.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.54217818424215	19.003809971551018	9.420838827932254	31.033173016274585
2	14.946419975663286	20.932294489393836	35.49658535376038	28.6247001811825
3	18.123117252639936	23.57460631311348	28.204395100938513	30.097881333308074
4	13.2138699607634	16.422045119506436	33.69806938287708	36.66601553685309
5	14.580879021295875	37.26618209258133	32.93488916186141	15.218049724261382
6	34.027775864776196	35.27917732973751	16.376341894091947	14.316704911394357
7	29.785080189488077	32.0034184432035	19.55712429319004	18.65437707411838
8	27.37527985890497	35.075729768034755	18.782828112399464	18.766162260660806
9	26.806505216141563	16.024114970660282	18.95385183251878	38.215527980679376
10	16.457169379813198	28.891997738489817	31.38695857869181	23.263874303005178
11	35.82944266749949	22.789207116793662	21.878035502705934	19.503314713000915
12	23.608059074722803	25.013308064652744	28.775134681746156	22.603498178878294
13	28.59692332483656	21.24773477664889	25.339236887873206	24.816105010641344
14	22.759614596174213	20.9236200066563	25.72318341961486	30.593581977554628
15	24.481243630810706	28.264718574389892	22.73732128816821	24.51671650663119
16	24.377592968096053	27.465344741446067	24.379452312801003	23.777609977656873
17	23.016030966988424	27.331700326486597	25.880690876211244	23.77157783031374
18	22.997116885451206	27.133472054311227	26.770715434562458	23.098695625675113
19	24.191965555450725	26.399499198731746	26.504177131590488	22.904358114227037
20	24.3683920208081	26.14750638201755	26.286268349806868	23.197833247367484
21	24.825768913689235	25.604755256892275	26.1679974048711	23.401478424547385
22	24.507291717597703	25.49773539091278	26.104741527428388	23.890231364061133
23	24.232137261855982	25.522737524231896	26.074002403390917	24.171122810521204
24	23.837975851673562	25.992834355152223	26.22963464164028	23.939555151533934
25	23.844710362646044	25.679982890474896	26.362846114309562	24.112460632569498
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	857.0
1	857.0
2	1009.0
3	1161.0
4	1161.0
5	1161.0
6	1538.5
7	1916.0
8	1916.0
9	1916.0
10	2609.0
11	3302.0
12	3302.0
13	3302.0
14	4929.0
15	6556.0
16	6556.0
17	6556.0
18	12804.0
19	19052.0
20	19052.0
21	19052.0
22	31688.0
23	44324.0
24	44324.0
25	44324.0
26	66611.0
27	88898.0
28	88898.0
29	88898.0
30	117234.0
31	145570.0
32	145570.0
33	145570.0
34	172692.5
35	199815.0
36	199815.0
37	199815.0
38	235317.5
39	270820.0
40	270820.0
41	270820.0
42	302765.0
43	334710.0
44	334710.0
45	334710.0
46	361671.5
47	388633.0
48	388633.0
49	388633.0
50	395119.5
51	401606.0
52	401606.0
53	401606.0
54	377303.0
55	353000.0
56	353000.0
57	353000.0
58	312615.0
59	272230.0
60	272230.0
61	272230.0
62	228583.5
63	184937.0
64	184937.0
65	184937.0
66	145295.5
67	105654.0
68	105654.0
69	105654.0
70	77318.0
71	48982.0
72	48982.0
73	48982.0
74	34894.0
75	20806.0
76	20806.0
77	20806.0
78	14068.0
79	7330.0
80	7330.0
81	7330.0
82	5070.5
83	2811.0
84	2811.0
85	2811.0
86	1882.5
87	954.0
88	954.0
89	954.0
90	611.5
91	269.0
92	269.0
93	269.0
94	159.5
95	50.0
96	50.0
97	50.0
98	30.5
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10054217020963044
2	0.0
3	0.04300588033966726
4	0.05533262586536852
5	0.07055168039710025
6	0.005164837510768686
7	2.7545800057432994E-4
8	0.003856412008040619
9	0.0
10	0.0
11	0.0027890122558150905
12	0.0
13	5.164837510768686E-4
14	0.05991211512491676
15	0.021589020795013108
16	1.7216125035895622E-4
17	0.0
18	0.0035465217573944978
19	0.003718683007753454
20	0.0
21	0.012120152025270517
22	0.0
23	8.263740017229899E-4
24	0.004097437758543158
25	0.18194000937934493
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2904254.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.981856968347337
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.357156059876914	18.98352075154855
2	13.643249243490258	8.726728917776368
3	7.306994381896587	7.010737475710024
4	4.589736133664035	5.871531381971947
5	3.1797763592160155	5.084757635588942
6	2.2653130060246376	4.346934992833015
7	1.7180108157734015	3.846162332609706
8	1.266136270393009	3.2394711281716773
9	1.0484637218718522	3.017863511046601
>10	5.333250226085176	29.227434748358288
>50	0.20834870110566653	4.504328963955624
>100	0.07836305348697178	4.279812903677067
>500	0.003684769206878444	0.8131010954555337
>1k	0.0014088823437848291	0.8282824023988651
>5k	1.0837556490652532E-4	0.21933175889777035
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6328	0.21788727845429498	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4022	0.13848650978874438	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3636	0.12519566126103296	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.886450014358248E-5	0.0	3.443225007179124E-5	0.0
3	0.0	1.0329675021537372E-4	0.0	3.443225007179124E-5	0.0
4	0.0	1.0329675021537372E-4	0.0	3.443225007179124E-5	0.0
5	0.0	1.0329675021537372E-4	0.0	3.443225007179124E-5	0.0
6	0.0	1.0329675021537372E-4	0.0	3.443225007179124E-5	0.0
7	0.0	1.0329675021537372E-4	0.0	3.443225007179124E-5	0.0
8	0.0	1.0329675021537372E-4	0.0	3.443225007179124E-5	0.0
9	0.0	1.0329675021537372E-4	0.0	3.443225007179124E-5	0.0
10	0.0	1.3772900028716497E-4	0.0	3.443225007179124E-5	0.0
11	0.0	1.3772900028716497E-4	0.0	3.443225007179124E-5	0.0
12	0.0	1.3772900028716497E-4	0.0	3.443225007179124E-5	0.0
13	0.0	2.410257505025387E-4	0.0	3.443225007179124E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCTG	50	0.0015007709	13.299204	5
ACGACTG	65	5.4587323E-5	13.153059	5
GGTATCA	1770	0.0	13.062523	1
GTGCGAT	115	8.8236084E-7	10.739227	11
GTCCTAG	240	0.0	10.704012	1
GTATTAC	125	2.1951018E-7	10.656438	1
GTATCAA	3695	0.0	10.300056	1
CCGCGGT	75	0.0026485557	10.133426	9
GTACGTT	75	0.002664878	10.125923	7
TCTATAC	235	0.0	10.111701	3
TAGGCCG	85	6.600246E-4	10.058221	5
TATAGAC	190	1.0422809E-9	9.505	3
CCGATAC	80	0.0044978783	9.504999	3
CGTGCGC	230	3.6379788E-12	9.500086	10
CGAGCCG	80	0.004517104	9.500086	14
AACGTAC	90	0.0011216056	9.4930525	6
TAATACC	225	2.7284841E-11	9.293777	4
TGGACCG	185	6.710252E-9	9.242689	5
AGGCCGT	155	4.3089676E-7	9.186825	6
ATTCGCC	105	4.4940296E-4	9.047702	11
>>END_MODULE