FastQCFastQC Report
Mon 15 Aug 2016
SRR2921180_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921180_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087805
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21960.10518223684683196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12250.012.4099321
TAGATAG1251.8439096E-811.3953445
GTATCAA26400.010.7250141
TGCACCG1201.5321766E-610.2874635
CGTTCGC951.6472425E-49.9983115
TTCGCGC951.6484376E-49.99759117
CGCAGGA1252.5745576E-69.8788042
TAGGACA1451.6282866E-79.8231014
CTAGGAC2054.1836756E-119.7277333
GTTCGCG900.00111557299.49839516
TGCGCCA1002.7612E-49.49816712
TTTACGG1002.765189E-49.49680218
ACGGTGC800.00452910649.4968018
CCTATAC1201.7089676E-59.49611953
TAATGCA1502.6662929E-79.4956654
GTATAAG2451.8189894E-129.3074491
GTTCTAG1951.6789272E-99.257731
CCCTAGA3100.09.19485951
ACCGTGC1451.7142884E-69.1693268
CATGGGT8000.09.0208824
Produced by FastQC (version 0.11.5)