##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921180_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2087805
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.971364183915643	31.0	27.0	31.0	10.0	34.0
2	26.72978175643798	31.0	27.0	31.0	10.0	34.0
3	26.563023845617767	31.0	26.0	31.0	10.0	34.0
4	30.462897157541054	35.0	31.0	37.0	10.0	37.0
5	30.240695850426643	35.0	30.0	37.0	10.0	37.0
6	30.50772366193203	35.0	31.0	37.0	8.0	37.0
7	30.478867039785804	35.0	31.0	37.0	8.0	37.0
8	30.050172789125423	35.0	30.0	37.0	2.0	37.0
9	31.685333160903436	37.0	31.0	39.0	2.0	39.0
10	31.0434053946609	37.0	30.0	39.0	2.0	39.0
11	31.4762010819976	37.0	31.0	39.0	2.0	39.0
12	31.18532573683845	37.0	30.0	39.0	2.0	39.0
13	31.18281161315353	37.0	30.0	39.0	2.0	39.0
14	32.27560476193897	38.0	31.0	40.0	2.0	41.0
15	31.927738941136745	38.0	31.0	40.0	2.0	41.0
16	31.841769705504106	38.0	31.0	40.0	2.0	41.0
17	32.158894628569236	38.0	31.0	40.0	2.0	41.0
18	32.202212850338036	38.0	31.0	40.0	2.0	41.0
19	31.91627522685308	38.0	31.0	40.0	2.0	41.0
20	31.781092582880106	38.0	31.0	40.0	2.0	41.0
21	31.728820938737094	38.0	31.0	40.0	2.0	41.0
22	30.982393949626523	38.0	29.0	40.0	2.0	41.0
23	31.079085450988	38.0	30.0	40.0	2.0	41.0
24	30.706591851250476	38.0	30.0	40.0	2.0	41.0
25	30.217346447584905	37.0	28.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	179199.0
3	11.0
4	1514.0
5	8120.0
6	9551.0
7	8508.0
8	7208.0
9	6731.0
10	6338.0
11	6118.0
12	5784.0
13	5825.0
14	5557.0
15	5853.0
16	6176.0
17	6810.0
18	7733.0
19	9691.0
20	12205.0
21	15001.0
22	18124.0
23	21456.0
24	24347.0
25	27660.0
26	30886.0
27	34685.0
28	38862.0
29	45806.0
30	53492.0
31	63906.0
32	78745.0
33	99541.0
34	127865.0
35	165727.0
36	217554.0
37	291684.0
38	433532.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.40029716770714	16.88211565008191	11.886324966926797	25.831262215284156
2	16.471634612850313	19.59615689653437	38.37419989256025	25.558008598055064
3	19.763014558293055	22.945096216951622	28.96396115872324	28.327928066032086
4	13.99940025905743	15.40409701624091	34.200035639557605	36.39646708514405
5	14.381983827291842	35.96322268107115	33.91986265508905	15.734930836547964
6	34.20942593446764	33.358575299371154	17.119896464831523	15.31210230132968
7	29.907009404436764	31.015481827700604	20.00983839926274	19.06767036859989
8	26.85618216478748	33.239182289321676	20.69638563450172	19.20824991138913
9	27.38788028876391	15.747941352434811	18.56114294637481	38.30303541242647
10	16.237869041933923	27.5467647505409	32.29592616311346	23.919440044411726
11	36.0611636147292	22.2863208661125	21.914311348877003	19.7382041702813
12	24.337601945161097	24.157302832573784	28.65524836154858	22.849846860716543
13	28.973339189939413	21.076022036039205	24.958578243410958	24.992060530610427
14	23.294957267045397	20.412177442959255	25.302095953099858	30.99076933689549
15	24.987174727867124	27.219227130659707	22.73965056821584	25.05394757325733
16	24.665741644858702	26.690169119922878	24.280816306192442	24.363272929025978
17	23.8793619367102	26.45391501593433	25.193207676270713	24.473515371084755
18	23.66069803138516	26.468364763844786	25.909619633641796	23.96131757112826
19	25.023257596443578	25.703979420260698	26.19571830284217	23.07704468045356
20	25.400768770886344	25.167090780881136	25.58203927399822	23.850101174234297
21	25.606871117677667	25.010276207202832	25.479771920898596	23.90308075422091
22	24.703981591295335	24.399580784285927	26.618856660031	24.27758096438774
23	24.967197274798934	24.74482378026318	25.69587526389487	24.592103681043017
24	24.58372533728307	25.14078610284523	25.802083193617058	24.47340536625464
25	24.15551254423712	24.804259467970738	26.431203759758155	24.60902422803399
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	996.0
1	996.0
2	1026.0
3	1056.0
4	1056.0
5	1056.0
6	1317.0
7	1578.0
8	1578.0
9	1578.0
10	1978.0
11	2378.0
12	2378.0
13	2378.0
14	3063.0
15	3748.0
16	3748.0
17	3748.0
18	6465.0
19	9182.0
20	9182.0
21	9182.0
22	15690.5
23	22199.0
24	22199.0
25	22199.0
26	35634.5
27	49070.0
28	49070.0
29	49070.0
30	68927.5
31	88785.0
32	88785.0
33	88785.0
34	112831.5
35	136878.0
36	136878.0
37	136878.0
38	164796.0
39	192714.0
40	192714.0
41	192714.0
42	218636.0
43	244558.0
44	244558.0
45	244558.0
46	266109.5
47	287661.0
48	287661.0
49	287661.0
50	292436.0
51	297211.0
52	297211.0
53	297211.0
54	281684.0
55	266157.0
56	266157.0
57	266157.0
58	235915.5
59	205674.0
60	205674.0
61	205674.0
62	172639.0
63	139604.0
64	139604.0
65	139604.0
66	108770.0
67	77936.0
68	77936.0
69	77936.0
70	57612.5
71	37289.0
72	37289.0
73	37289.0
74	25912.5
75	14536.0
76	14536.0
77	14536.0
78	10097.0
79	5658.0
80	5658.0
81	5658.0
82	3829.0
83	2000.0
84	2000.0
85	2000.0
86	1358.0
87	716.0
88	716.0
89	716.0
90	450.0
91	184.0
92	184.0
93	184.0
94	105.5
95	27.0
96	27.0
97	27.0
98	18.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036785044580312815
2	0.05048364191100222
3	0.01604555981042291
4	0.011351634850955908
5	0.01024999940128508
6	5.747663215673877E-4
7	0.0032091119620845814
8	0.0011974298365987246
9	0.007088784632664449
10	0.003257009155548531
11	0.010728971335924571
12	0.007807242534623684
13	0.006418223924169163
14	0.014656541199968388
15	0.006226635150313368
16	0.03098948417117499
17	0.03841354915808708
18	0.02150583986531309
19	0.01508761594114393
20	0.015183410328071826
21	0.02198481179995258
22	0.004646027766003051
23	0.015614485069247367
24	0.011543223624811704
25	0.4693445987532361
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2087805.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.293593192369656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.98530043442761	38.89648910375266
2	12.623747101062962	13.455296851348105
3	5.432826104129385	8.686044728350737
4	2.954422713398582	6.298072088246438
5	1.7092227711389882	4.5545311520107985
6	1.0895998610397535	3.4841215042029074
7	0.7428852151461467	2.7713715711237326
8	0.49708055572658516	2.119296713658373
9	0.3740513297057806	1.7941085458564328
>10	1.5145457482518614	13.16274841073651
>50	0.05452275635773479	1.9455741366978192
>100	0.01975595961797668	1.890796671910339
>500	0.0015759386338249721	0.600757163857666
>1k	4.6351136288969215E-4	0.34079135824754475
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2196	0.10518223684683196	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	4.789719346394898E-5	0.0
11	0.0	0.0	0.0	4.789719346394898E-5	0.0
12	0.0	0.0	0.0	4.789719346394898E-5	0.0
13	0.0	0.0	0.0	4.789719346394898E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1225	0.0	12.409932	1
TAGATAG	125	1.8439096E-8	11.395344	5
GTATCAA	2640	0.0	10.725014	1
TGCACCG	120	1.5321766E-6	10.287463	5
CGTTCGC	95	1.6472425E-4	9.99831	15
TTCGCGC	95	1.6484376E-4	9.997591	17
CGCAGGA	125	2.5745576E-6	9.878804	2
TAGGACA	145	1.6282866E-7	9.823101	4
CTAGGAC	205	4.1836756E-11	9.727733	3
GTTCGCG	90	0.0011155729	9.498395	16
TGCGCCA	100	2.7612E-4	9.498167	12
TTTACGG	100	2.765189E-4	9.496802	18
ACGGTGC	80	0.0045291064	9.496801	8
CCTATAC	120	1.7089676E-5	9.4961195	3
TAATGCA	150	2.6662929E-7	9.495665	4
GTATAAG	245	1.8189894E-12	9.307449	1
GTTCTAG	195	1.6789272E-9	9.25773	1
CCCTAGA	310	0.0	9.1948595	1
ACCGTGC	145	1.7142884E-6	9.169326	8
CATGGGT	800	0.0	9.020882	4
>>END_MODULE