FastQCFastQC Report
Mon 15 Aug 2016
SRR2921180_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921180_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087805
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40790.1953726521394479No Hit
GGTATCAACGCAGAGTACTTTTTTT25280.12108410507686303No Hit
TATCAACGCAGAGTACTTTTTTTTT21830.10455957333180062No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACG602.522504E-514.2776811
GGTATCA15150.013.382381
CTAGGGT1155.284164E-912.3981084
TAGACCG550.003070211312.09035
TATGCGA1157.0873284E-811.56407912
CGTATGC1201.27764E-711.08224310
TAGGACG1053.4447366E-610.8631044
GTTCTAG1252.1818596E-710.6606671
GTGTTAA1701.3460522E-1010.6382711
ACCGTAT1252.247707E-710.6376788
TTAATCC1803.45608E-1110.5613514
GTATGCG1355.6978024E-810.55451811
TACCGTA1355.7501893E-810.5479457
CCCCTAG1105.9088234E-610.3837681
CCTAGAC1752.3283064E-1010.3199483
AGGATCG1303.8452708E-710.2302535
CCGTCCG750.002650383210.1323379
ATCGATC856.6082046E-410.0566298
TTCGCCT2650.010.0314617
AGGAGTG3050.09.966715
Produced by FastQC (version 0.11.5)