##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921180_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2087805
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.50614401249159	31.0	30.0	33.0	26.0	34.0
2	29.760193121484047	31.0	30.0	34.0	26.0	34.0
3	29.90104008755607	31.0	30.0	34.0	26.0	34.0
4	33.5855767181322	35.0	35.0	37.0	29.0	37.0
5	33.11849142999466	35.0	35.0	37.0	27.0	37.0
6	33.07814762393998	36.0	35.0	37.0	26.0	37.0
7	32.92999106717342	36.0	35.0	37.0	26.0	37.0
8	32.863999750934596	36.0	35.0	37.0	26.0	37.0
9	34.24121026628445	38.0	35.0	39.0	26.0	39.0
10	34.09987091706361	38.0	34.0	39.0	25.0	39.0
11	34.12856420978013	38.0	35.0	39.0	25.0	39.0
12	33.99275075976923	38.0	34.0	39.0	25.0	39.0
13	33.9629759484243	38.0	34.0	39.0	25.0	39.0
14	34.9041433467206	39.0	34.0	41.0	24.0	41.0
15	34.89050462088174	39.0	34.0	41.0	24.0	41.0
16	34.87056741410237	39.0	34.0	41.0	24.0	41.0
17	34.84315920308649	39.0	34.0	41.0	24.0	41.0
18	34.79407846997205	39.0	34.0	41.0	24.0	41.0
19	34.83027964776404	39.0	34.0	41.0	23.0	41.0
20	34.765112163252795	39.0	34.0	41.0	23.0	41.0
21	34.69430574215504	39.0	34.0	41.0	22.0	41.0
22	34.60916033824998	39.0	34.0	41.0	20.0	41.0
23	34.481760030271026	39.0	34.0	41.0	18.0	41.0
24	34.3973589487524	39.0	34.0	41.0	18.0	41.0
25	34.258997847021156	39.0	34.0	41.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69722.0
3	4.0
4	1201.0
5	9710.0
6	10713.0
7	8236.0
8	6245.0
9	5397.0
10	4888.0
11	4483.0
12	4149.0
13	3922.0
14	3833.0
15	3815.0
16	3960.0
17	4004.0
18	4477.0
19	5333.0
20	6755.0
21	8767.0
22	10700.0
23	13442.0
24	15698.0
25	18129.0
26	20612.0
27	23372.0
28	26819.0
29	32317.0
30	41379.0
31	52981.0
32	66984.0
33	85990.0
34	112584.0
35	149499.0
36	203072.0
37	306177.0
38	738436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.01377251968787	17.869370827190238	8.950579548587388	32.1662771045345
2	15.13374093844971	20.819808363328953	34.64394423808737	29.40250646013397
3	18.66531795120474	23.351353475258325	27.320113238431297	30.663215335105637
4	13.738308396197343	16.18544008641615	32.4217272974298	37.654524219956706
5	15.229541960986568	36.85632076199716	31.993134279854306	15.92100299716197
6	34.62977297417435	34.21754741599815	16.326306754061424	14.826372855766081
7	30.378584569521166	31.49583557452499	18.90207074134868	19.22350911460516
8	28.18968754550401	34.211975889579485	18.406229163952393	19.192107400964108
9	27.493899094982527	15.743711697213103	18.23158772011754	38.53080148768683
10	16.976585456975148	28.255320779478925	30.51673887168581	24.25135489186011
11	36.36421550477528	22.340809232607075	21.459709129193367	19.835266133424277
12	24.621456505756043	24.35711189502851	28.02733013859053	22.994101460624915
13	29.163531372183684	20.902591210127078	24.77338466674554	25.160492750943703
14	23.603189368898143	20.36426515067645	24.911852396076405	31.120693084349
15	25.2096713767071	27.34806550427966	22.26502576996082	25.17723734905242
16	25.30529978196978	26.6064880274971	23.520536607235233	24.567675583297888
17	24.031027801925948	26.487435368724572	24.99634783899837	24.485188990351112
18	23.87015500162857	26.32575249554538	25.927854309964935	23.876238192861113
19	25.207487810824574	25.559691394363114	25.701808629826033	23.53101216498628
20	25.405102488019715	25.148421428246408	25.523983322197235	23.92249276153664
21	25.44773609797739	24.831592975719303	25.51690968439791	24.203761241905397
22	25.52269009797371	24.574660947741766	25.17763871625942	24.725010238025103
23	25.030307239490657	24.693347255584268	25.396053712427285	24.88029179249779
24	24.71924751388255	25.264634215854535	25.39736445923802	24.618753811024895
25	24.71784005186148	24.84998893960834	25.519466529239498	24.912704479290674
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	704.0
1	704.0
2	757.0
3	810.0
4	810.0
5	810.0
6	990.5
7	1171.0
8	1171.0
9	1171.0
10	1450.5
11	1730.0
12	1730.0
13	1730.0
14	2218.0
15	2706.0
16	2706.0
17	2706.0
18	5250.5
19	7795.0
20	7795.0
21	7795.0
22	14093.0
23	20391.0
24	20391.0
25	20391.0
26	32974.5
27	45558.0
28	45558.0
29	45558.0
30	63351.0
31	81144.0
32	81144.0
33	81144.0
34	103242.5
35	125341.0
36	125341.0
37	125341.0
38	152331.0
39	179321.0
40	179321.0
41	179321.0
42	206044.0
43	232767.0
44	232767.0
45	232767.0
46	257203.5
47	281640.0
48	281640.0
49	281640.0
50	292774.0
51	303908.0
52	303908.0
53	303908.0
54	291650.0
55	279392.0
56	279392.0
57	279392.0
58	249186.5
59	218981.0
60	218981.0
61	218981.0
62	184951.5
63	150922.0
64	150922.0
65	150922.0
66	118515.5
67	86109.0
68	86109.0
69	86109.0
70	63753.0
71	41397.0
72	41397.0
73	41397.0
74	28731.5
75	16066.0
76	16066.0
77	16066.0
78	11297.0
79	6528.0
80	6528.0
81	6528.0
82	4441.0
83	2354.0
84	2354.0
85	2354.0
86	1577.5
87	801.0
88	801.0
89	801.0
90	512.5
91	224.0
92	224.0
93	224.0
94	132.0
95	40.0
96	40.0
97	40.0
98	22.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10202102207821132
2	0.0
3	0.04219742744173905
4	0.055464950031252916
5	0.07174999580899558
6	0.0054123828614262345
7	9.579438692789796E-5
8	0.0036880838967240717
9	0.0
10	0.0
11	0.0035443923163322247
12	0.0
13	7.663550954231837E-4
14	0.05977569744300833
15	0.02351752199079895
16	0.0010058410627429286
17	0.0
18	0.004071261444435663
19	0.004406541798683306
20	0.0
21	0.014512849619576542
22	0.0
23	9.579438692789797E-4
24	0.005364485667962286
25	0.18162615761529452
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2087805.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.923868455812915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.286383024041214	25.89923613920055
2	15.117052005458683	12.372964954221482
3	7.391566069166462	9.074744324910554
4	4.187642212187262	6.854980761262436
5	2.599517817698951	5.319116261002687
6	1.7128573198493986	4.2058048584655845
7	1.1713078743372631	3.35541145594452
8	0.8670257881539941	2.8385639441769257
9	0.632745901157151	2.3304969022419044
>10	2.874184124886591	19.68303995647748
>50	0.11320790306393518	3.1492565108538693
>100	0.04249795941757917	3.1621951512897004
>500	0.0024780003591326474	0.7123766140465875
>1k	0.0015340002223202037	1.0418121659057094
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4079	0.1953726521394479	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2528	0.12108410507686303	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2183	0.10455957333180062	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	4.789719346394898E-5	0.0
11	0.0	0.0	0.0	4.789719346394898E-5	0.0
12	0.0	0.0	0.0	4.789719346394898E-5	0.0
13	0.0	0.0	0.0	4.789719346394898E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTACG	60	2.522504E-5	14.277681	1
GGTATCA	1515	0.0	13.38238	1
CTAGGGT	115	5.284164E-9	12.398108	4
TAGACCG	55	0.0030702113	12.0903	5
TATGCGA	115	7.0873284E-8	11.564079	12
CGTATGC	120	1.27764E-7	11.082243	10
TAGGACG	105	3.4447366E-6	10.863104	4
GTTCTAG	125	2.1818596E-7	10.660667	1
GTGTTAA	170	1.3460522E-10	10.638271	1
ACCGTAT	125	2.247707E-7	10.637678	8
TTAATCC	180	3.45608E-11	10.561351	4
GTATGCG	135	5.6978024E-8	10.554518	11
TACCGTA	135	5.7501893E-8	10.547945	7
CCCCTAG	110	5.9088234E-6	10.383768	1
CCTAGAC	175	2.3283064E-10	10.319948	3
AGGATCG	130	3.8452708E-7	10.230253	5
CCGTCCG	75	0.0026503832	10.132337	9
ATCGATC	85	6.6082046E-4	10.056629	8
TTCGCCT	265	0.0	10.03146	17
AGGAGTG	305	0.0	9.96671	5
>>END_MODULE