FastQCFastQC Report
Mon 15 Aug 2016
SRR2921179_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921179_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3481143
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16400.011.9346141
GTATCAA34800.09.9927931
GGACCGT1252.5838544E-69.8765726
GTCCTAG4100.09.7330841
GTCTTAG4250.09.6131241
GTACTAG2303.6379788E-129.5013441
GTATAGG2451.8189894E-129.3074391
GTGTAGG4150.09.1579221
CAGGACT6250.08.8123074
CGTCTAT1201.7621377E-48.7095651
TTATACT4650.08.5770514
ACCGTCC1451.697757E-58.5142858
GTGCTAA3350.08.5086661
CCTACAC4100.08.3383283
CTTAGAC3650.08.3256373
TGAACCG1606.488486E-68.3092575
GTATAAT5850.08.2832221
GTCTATA3100.08.2753641
GTATAAA5400.08.2696891
GTACTAC2803.45608E-118.144011
Produced by FastQC (version 0.11.5)