##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921179_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3481143
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.448307639186325	31.0	28.0	31.0	15.0	34.0
2	27.046938031560323	31.0	27.0	31.0	10.0	34.0
3	26.921162962854442	31.0	27.0	31.0	10.0	34.0
4	30.78074442790773	35.0	31.0	37.0	12.0	37.0
5	30.46035712982776	35.0	30.0	37.0	10.0	37.0
6	30.742009161933307	35.0	31.0	37.0	11.0	37.0
7	30.78240221674318	35.0	32.0	37.0	10.0	37.0
8	30.47218542875142	35.0	31.0	37.0	10.0	37.0
9	32.0777307912947	37.0	32.0	39.0	10.0	39.0
10	31.455889918914565	37.0	30.0	39.0	8.0	39.0
11	31.888426875885305	37.0	32.0	39.0	8.0	39.0
12	31.59148159096021	37.0	31.0	39.0	7.0	39.0
13	31.591496241320737	37.0	31.0	39.0	2.0	39.0
14	32.69386405557025	38.0	32.0	40.0	2.0	41.0
15	32.3524356798902	38.0	31.0	40.0	2.0	41.0
16	32.279765008217126	38.0	31.0	40.0	2.0	41.0
17	32.58889134976644	38.0	32.0	40.0	2.0	41.0
18	32.645203888492944	38.0	32.0	40.0	2.0	41.0
19	32.35515317813718	38.0	31.0	40.0	2.0	41.0
20	32.22827904512972	38.0	31.0	40.0	2.0	41.0
21	32.17771030951616	38.0	31.0	40.0	2.0	41.0
22	31.414059117939136	38.0	30.0	40.0	2.0	41.0
23	31.513562355812443	38.0	30.0	40.0	2.0	41.0
24	31.13936399625066	38.0	30.0	40.0	2.0	41.0
25	30.650943095414352	38.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	264883.0
3	10.0
4	2173.0
5	12441.0
6	14794.0
7	13056.0
8	11047.0
9	10299.0
10	9785.0
11	9407.0
12	9105.0
13	8939.0
14	9084.0
15	9134.0
16	9817.0
17	10650.0
18	12602.0
19	15083.0
20	19264.0
21	24267.0
22	29948.0
23	35105.0
24	40967.0
25	46402.0
26	52342.0
27	58966.0
28	65384.0
29	77531.0
30	90946.0
31	109054.0
32	136245.0
33	172766.0
34	221568.0
35	285242.0
36	374269.0
37	492266.0
38	716302.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.1870963570664	17.946968771776064	12.373876624449537	25.492058246707998
2	15.400771849230738	20.09190569965937	39.79866277178255	24.70865967932734
3	18.589752982246864	23.636437812025864	30.345814511585523	27.42799469414175
4	13.483180606481266	15.890564126460536	36.01296011615611	34.6132951509021
5	13.64170189245357	36.97084471216745	34.83368276624461	14.553770629134375
6	33.27061405524659	34.827814036861696	17.468659511881235	14.432912396010481
7	29.02695278644464	31.633377985086526	21.122127380736586	18.217541847732257
8	25.630493984625463	35.40685649281258	20.730261872752134	18.232387649809827
9	26.754273195510525	16.11885055285306	19.400202761148343	37.72667349048807
10	15.218915710032885	28.639146370785994	33.739859002028986	22.402078917152128
11	35.23837322548079	22.94660828165463	22.467638205218226	19.347380287646352
12	23.005945677605	24.856960143045605	30.117862084181784	22.019232095167617
13	28.3336273204634	21.644136910285276	25.51005809453822	24.512177674713104
14	22.253662755035343	21.123613583114622	26.48598176487404	30.136741896975995
15	24.18015514805944	28.40373543529853	23.429350694196067	23.986758722445963
16	23.807383764132407	27.642479200441823	25.344714926660615	23.205422108765152
17	22.93668015291503	27.69474237472344	26.269562316209278	23.09901515615225
18	22.82918408668352	27.601208011306642	26.80768559985818	22.761922302151657
19	23.94622508534492	26.912108377277132	26.926243944259898	22.21542259311805
20	24.316737128352315	26.454405327794085	26.544762922644416	22.684094621209184
21	24.58482490082315	26.139014521036234	26.253110634728948	23.02304994341166
22	23.708624681838813	25.583491815429166	27.487862473929457	23.220021028802567
23	24.09404763922739	25.906598797512743	26.417255091694898	23.582098471564972
24	23.497434170720872	26.170223279722222	26.753862534065973	23.578480015490932
25	23.322956686444623	25.92127368435025	27.183554111212494	23.572215517992632
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1215.0
1	1215.0
2	1306.5
3	1398.0
4	1398.0
5	1398.0
6	1743.5
7	2089.0
8	2089.0
9	2089.0
10	2941.5
11	3794.0
12	3794.0
13	3794.0
14	6490.0
15	9186.0
16	9186.0
17	9186.0
18	17456.0
19	25726.0
20	25726.0
21	25726.0
22	43355.0
23	60984.0
24	60984.0
25	60984.0
26	90966.5
27	120949.0
28	120949.0
29	120949.0
30	157841.5
31	194734.0
32	194734.0
33	194734.0
34	235347.0
35	275960.0
36	275960.0
37	275960.0
38	318564.0
39	361168.0
40	361168.0
41	361168.0
42	396388.0
43	431608.0
44	431608.0
45	431608.0
46	454262.0
47	476916.0
48	476916.0
49	476916.0
50	475030.0
51	473144.0
52	473144.0
53	473144.0
54	433779.0
55	394414.0
56	394414.0
57	394414.0
58	342345.5
59	290277.0
60	290277.0
61	290277.0
62	238060.5
63	185844.0
64	185844.0
65	185844.0
66	142594.0
67	99344.0
68	99344.0
69	99344.0
70	72341.0
71	45338.0
72	45338.0
73	45338.0
74	31324.0
75	17310.0
76	17310.0
77	17310.0
78	11983.5
79	6657.0
80	6657.0
81	6657.0
82	4410.0
83	2163.0
84	2163.0
85	2163.0
86	1428.0
87	693.0
88	693.0
89	693.0
90	442.0
91	191.0
92	191.0
93	191.0
94	110.0
95	29.0
96	29.0
97	29.0
98	20.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03211588837344516
2	0.049035618473587554
3	0.013271503066665172
4	0.008905121105338103
5	0.0076124422352083785
6	6.607025336218593E-4
7	0.0037344056248192043
8	0.0018097504181816145
9	0.005745239422798776
10	0.002872619711399388
11	0.009795633215871914
12	0.008474228148628195
13	0.0076124422352083785
14	0.014305646162768951
15	0.006032501393938715
16	0.029329447253387753
17	0.037401508642420034
18	0.020453052345163644
19	0.016115396580950566
20	0.01516743207618877
21	0.021372290652811446
22	0.005831418014140758
23	0.015052527287732792
24	0.01140430025425557
25	0.45011078257917014
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3481143.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.360611557926845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.24873448998432	34.67520495596054
2	12.904814568539619	12.739790782895192
3	5.960994543190788	8.827150084360863
4	3.3415195061180785	6.597577854189201
5	2.0501309094559765	5.059785773227787
6	1.3656301572258094	4.044500383356824
7	0.9109305249006794	3.1474861457116585
8	0.6737202204076472	2.6604193678610186
9	0.48526297223290793	2.1557589368250247
>10	1.9790976046863589	15.579851122229266
>50	0.05655055756896702	1.886897368977674
>100	0.02094286911980481	1.828149994589192
>500	0.0011936261227907191	0.4309565543082486
>1k	4.774504462060769E-4	0.3664706755073905
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	5.7452394227987763E-5	0.0
8	0.0	0.0	0.0	5.7452394227987763E-5	0.0
9	0.0	0.0	0.0	2.0108337979795718E-4	0.0
10	0.0	0.0	0.0	2.2980957691195105E-4	0.0
11	0.0	0.0	0.0	2.872619711399388E-4	0.0
12	0.0	0.0	0.0	2.872619711399388E-4	0.0
13	0.0	0.0	0.0	3.1598816825393266E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1640	0.0	11.934614	1
GTATCAA	3480	0.0	9.992793	1
GGACCGT	125	2.5838544E-6	9.876572	6
GTCCTAG	410	0.0	9.733084	1
GTCTTAG	425	0.0	9.613124	1
GTACTAG	230	3.6379788E-12	9.501344	1
GTATAGG	245	1.8189894E-12	9.307439	1
GTGTAGG	415	0.0	9.157922	1
CAGGACT	625	0.0	8.812307	4
CGTCTAT	120	1.7621377E-4	8.709565	1
TTATACT	465	0.0	8.577051	4
ACCGTCC	145	1.697757E-5	8.514285	8
GTGCTAA	335	0.0	8.508666	1
CCTACAC	410	0.0	8.338328	3
CTTAGAC	365	0.0	8.325637	3
TGAACCG	160	6.488486E-6	8.309257	5
GTATAAT	585	0.0	8.283222	1
GTCTATA	310	0.0	8.275364	1
GTATAAA	540	0.0	8.269689	1
GTACTAC	280	3.45608E-11	8.14401	1
>>END_MODULE