FastQCFastQC Report
Mon 15 Aug 2016
SRR2921179_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921179_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3481143
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53570.15388623793966522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20150.012.6527511
TCGCGCA802.8876639E-511.86737818
ACCGCTC1201.2796954E-711.0816318
CGACAGT951.3718036E-510.9924676
GTTCGCG803.795658E-410.6806416
CTATTAG2650.010.4106381
CCGTCCA2350.010.1065749
GTATCAA43050.010.0766681
CTACGGG951.6372198E-410.0047913
TAGGACA4600.09.9177934
CGTGCGC2600.09.86557210
TACGGGT1201.692141E-59.5045514
TAGGACT4300.09.5045514
CCTATAC2900.09.5045513
CTAGACA4150.09.39003854
ATAATAC3150.09.3536853
GTATAGG2451.8189894E-129.3190241
GTCCTAG2900.09.1851291
TACACCG2600.09.1334765
GTATAGA3450.09.0995541
Produced by FastQC (version 0.11.5)